[Effect of Naotaifang on microglial polarization and glial scar following cerebral ischemia reperfusion injury].

• DOI: 10.19540/j.cnki.cjcmm.20230921.401
• Publication Date: 2024-02-01
• Authors: Jiyong Liu; Wenli Zhang; Jun Liao; Lijuan Hu; Guo-Zuo Wang; Jinwen Ge; Zhigang Mei

This study aims to investigate the effect of Naotaifang(NTF) on proteins associated with microglial polarization and glial scar in rat model cerebral ischemia reperfusion injury(CIRI). The CIRI was established by middle artery occlusion/reperfusion. 48 successfully modeled rats were randomized into 7 d, 14 NTF d groups(n=12). In addition, 12 SD selected as sham group. group administrated suspension at 27 g·kg~(-1)·d~(-1) gavage, sham, groups same volume normal saline every day gavage for days, respectively. After intervention, Longa score evaluated. infarct measured 2,3,5-triphenyl-2H-tetrazolium chloride(TTC) staining. Morris water maze open field tests carried out evaluate spatial learning, memory, cognitive function, anxiety degree rats. Hematoxylin-eosin(HE) staining employed observe morphological structure damage brain tissue. immunofluorescence assay measure expression fibrillary acidic protein(GFAP) scar. Western blot determine protein levels GFAP, neurocan, phosphacan, CD206, arginase-1(Arg-1), interleukin(IL)-1β, IL-6, IL-4. Compared showed infarction different degrees, severe pathological injury cortex hippocampus, neurological impairment, reduced learning dysfunction, anxiety, astrocyte hyperplasia, thickening penumbra scar, up-regulated IL-1β, Arg-1(P<0.01). group, improved nerve volume, thinned down-regulated IL-4, Arg-1(P<0.05 or P<0.01). exerts a neuroprotective inducing M2 microglia, inhibiting inflammatory response, reducing formation