Asymptomatic carriage of Campylobacter jejuni is highly prevalent in chicken flocks. Thus, we investigated whether chronic affects intestinal functions despite the absence clinical symptoms. An experiment was carried out which commercial chickens were orally infected with C. (1 × 10 8 CFU/bird) at 14 days life. Changes ion transport and barrier function assessed by short-circuit current ( I sc ) transepithelial conductance G t Ussing chambers. increased cecum colon Campylobacter-infected 7 d...

Abstract Colisepticaemia caused by avian pathogenic Escherichia coli (APEC) is a challenging disease due to its high economic importance in poultry, dubious pathogenesis and potential link with zoonosis food safety. The existing vitro studies can’t define hallmark traits of APEC isolates, suggesting paradigm shift towards host response understand pathogenesis. This study investigated the comprehensive pathological microbial progression colisepticaemia, transmission E. into eggs using novel...

The present report documents an outbreak of adenoviral gizzard erosion in 22 broiler flocks Germany. clinical picture was characterized by uneven growth affected broilers that resulted considerably lower than average weight at slaughtering. Fowl adenovirus serotype 1 (FAdV-1) isolated from lesions and histological examinations demonstrated FAdV-1-positive intranuclear inclusion bodies epithelial cells in-situ hybridization. Birds all originated one breeder farm. During production birds,...

Numerous cases of tenosynovitis appeared in France causing high morbidity free‐range and standard broilers. The main clinical findings were lameness, stunting non‐uniform bodyweights. Although the natural mortality was low, economic losses due to birds that had be removed from flock prematurely, downgrading carcases lower average weights at slaughter substantial. Postmortem examinations, bacteriological, virological serological examination confirmed aetiology avian orthoreovirus...

In the present study, inclusion body hepatitis (IBH) was experimentally induced by oral inoculation of two groups specific pathogen-free (SPF) broilers and SPF layers at day-old with either a fowl aviadenovirus (FAdV)-D or FAdV-E strain. A substantial variation in degree susceptibility observed mortalities 100 96% D infected broiler groups, respectively, whereas only 20 8% were noticed. Significant changes clinical chemistry analytes all birds together histopathological lesions indicated...

In the present study, we report occurrence of several outbreaks hepatitis in flocks young pheasants France, between 2017 and 2021. The disease was characterized by prostration, apathy a median cumulative mortality 12%, with birds presenting multifocal to coalescing necrotizing on necropsy. Severe extensive areas degeneration necrosis were observed liver, degenerative hepatocytes large amphophilic acidophilic intranuclear inclusion bodies. Transmission electron microscopy examination liver...

In the present investigation, pathogenicity and transmission of a mono-eukaryotic culture Histomonas meleagridis for commercial turkeys specific pathogen free (SPF) chickens is described first time. Two separate trials with same kind experimental design were performed, one SPF chickens. each experiment, two different groups included, which housed in rooms. The group contained four control birds, whereas second consisted 10 infected in-contact birds. birds via cloaca at 14 days age 380 000...

Several outbreaks of Riemerella anatipestifer in commercial geese occurred within a short time period. A serious disease was recognized the affected birds, mainly characterized by depression and severe neurologic disturbances. The morbidity ranged from 20 to 30% mortality 5 20%. Generally, clinical signs started at age 8 10 days. Post-mortem examination revealed fibrinous pericarditis, perihepatitis airsacculitis all birds. Some birds also had synovitis tibio-tarsal joints oedematous...

The effect of attenuated Histomonas meleagridis on pullets was investigated and the protection vaccinated adult laying hens against a severe challenge studied in same experimental setting. Four groups 25 were set up at 18 weeks life birds two with vitro-attenuated H. meleagridis. Chickens (vaccinated non-vaccinated) challenged 5 later virulent histomonads, while remaining retained until termination study 11 post vaccination. Vaccination did not have any impact their subsequent performance....

Abstract In the present study day-old specific-pathogen-free (SPF) and commercial broilers with maternally derived fowl adenovirus serotype 1 (FAdV-1) antibodies were orally infected a European “pathogenic” FAdV-1, isolated from showing signs of gizzard erosion. During experiment, observed weighed daily up to 17 days post infection (dpi). Clinically, both groups showed significant decrease weight compared respective negative control groups. Birds examined by necropsy at 3, 7, 10, 14 dpi....

Histomonas meleagridis is the aetiological agent of histomonosis or "blackhead disease". Histomonosis special importance today, because there no effective treatment to prevent its occurrence with considerable losses for poultry industry. Despite only a few molecular studies have yet been performed investigate degree genetic diversity between different isolates this parasite. In present study collection well defined samples, previously shown positive DNA H. meleagridis, was used relatedness...

Evaluation of reference genes for expression studies in chickens and turkeys is very much limited unavailable various infectious models. In this study, eight candidate HMBS, HPRT1, TBP, VIM, TFRC, RPLP0, RPL13 RPS7 were evaluated by five different algorithms (GeNorm, NormFinder, BestKeeper©, delta CT, RefFinder) to assess their stability. order analyze a broad variation tissues, spleen, liver, caecum caecal tonsil aged specific pathogen free (SPF) layer commercial turkeys, uninfected or...

Abstract A recombinant fowl adenovirus (FAdV) fiber protein, derived from a FAdV-8a strain, was tested for its efficacy to protect chickens against inclusion body hepatitis (IBH). FAdV-E field isolates belonging both homotypic (FAdV-8a) and heterotypic (-8b) serotype were used as challenge. Mechanisms underlying fiber-induced protective immunity investigated by fiber-based ELISA, virus neutralization assays flow cytometry of peripheral blood mononuclear cells, monitoring the temporal...

Two separate bird trials were performed to establish a reliable route of infection for Gallibacterium anatis in chickens, comparing intranasal (i.n.) and intravenous (i.v.) applications. Additionally, three mutually divergent isolates from geographical locations, as shown by MALDI-TOF-MS partial rpoB gene sequence analysis, compared. In the first trial, birds infected with one selected i.v. or i.n. route. Subsequently, killed 3, 12 24 h post following while at 7 10 days (dpi) case along...

Pathogenicity of Gallibacterium anatis was studied in specific pathogen free layers a controlled environment, applying the intranasal route for experimental infection. At 30 weeks, 37 hens were infected with 0.4 ml 1.53 × 10(8) colony-forming units/ml suspension G. strain 07990 whereas equal numbers left uninfected control. Following infection, clinical signs and number weight eggs recorded daily until 5 weeks post Three birds from each group killed at 3, 7, 10, 28 38 days infection (d.p.i.)...

In the present study, clinical chemistry was applied to assess pathogenesis and progression of experimentally induced inclusion body hepatitis (IBH). For this, five fowl aviadenovirus (FAdV) strains from recent IBH field outbreaks were used orally inoculate different groups day-old specific pathogen-free chickens, which weighed, sampled examined during necropsy by sequential killing. Mortalities 50% 30% recorded in two between 6 9 days post-infection (dpi), along with a decreased weight 23%...

Histomonosis in chickens often appears together with colibacillosis the field. Thus, we have experimentally investigated consequences of co-infection birds Histomonas meleagridis and avian pathogenic Escherichia coli (APEC) on pathology, host microbiota bacterial translocation from gut. Commercial chicken layers were infected via oral cloacal routes lux-tagged APEC or without H. whereas negative controls left uninfected. Except one bird, which died due to colibacillosis, no clinical signs...