A5103150916- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Herpesvirus Infections and Treatments
- Microbial infections and disease research
- Respiratory viral infections research
- Infectious Diseases and Mycology
- Veterinary Oncology Research
- Influenza Virus Research Studies
- Helminth infection and control
- Diagnosis and treatment of tuberculosis
- Listeria monocytogenes in Food Safety
- T-cell and B-cell Immunology
- Sarcoma Diagnosis and Treatment
- Respiratory Support and Mechanisms
- Toxoplasma gondii Research Studies
- Animal Nutrition and Physiology
- Immune Cell Function and Interaction
- Prion Diseases and Protein Misfolding
- Veterinary medicine and infectious diseases
University of Córdoba
2016-2025
Cordoba University
2009-2024
University of Veterinary Medicine Vienna
2015-2019
Pigs are natural hosts for influenza A viruses and play a critical role in epidemiology. However, little is known about their influenza-evoked T-cell response. We performed thorough analysis of both the local systemic response virus-infected pigs, addressing kinetics phenotype as well multifunctionality (gamma interferon [IFN-γ], tumor necrosis factor alpha [TNF-α], interleukin-2 [IL-2]) cross-reactivity. total 31 pigs were intratracheally infected with an H1N2 swine virus (FLUAVsw)...
Unlike mice and humans, porcine γδ T cells represent a prominent subset of in blood secondary lymphatic organs. GATA-3, T-bet Eomesodermin (Eomes) are transcription factors with crucial functions T-cell development functional differentiation, but their expression has not been investigated so far. We analyzed the these thymocytes, mature from blood, spleen, lymph nodes lung tissue as well vitro stimulated on protein level by flow cytometry. GATA-3 was present more than 80% all γδ-thymocytes....
Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important infectious diseases for pig industry worldwide. The disease was firstly reported in 1987 became endemic many countries. Since then, outbreaks caused by strains high virulence have been several times Asia, America Europe. Interstitial pneumonia, microscopically characterised thickened alveolar septa, hallmark lesion PRRS. However, suppurative bronchopneumonia proliferative necrotising pneumonia are...
Porcine reproductive and respiratory syndrome virus (PRRSV) induces a dysregulation on the innate adaptive immune responses. T-cell activation requires proper interaction precise balance between costimulatory coinhibitory molecules, commonly known as checkpoints. This study aims to evaluate expression of checkpoints in lung tracheobronchial lymph node from piglets infected with two PRRSV-1 strains different virulence during early stage infection. Seventy 4-week-old were grouped into three...
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection is characterized by persisting in lungs lymphoid tissue, resulting systemic depletion. The aim of this study was to correlate the histological changes, viral antigen expression apoptosis phenomena tonsil, medial retropharyngeal mediastinal lymph nodes 12 pigs inoculated with a type 2 PRRSV isolate (Chilean strain 2402). Apoptosis were observed mainly lymphocytes secondly macrophages tonsils animals, showing peak both...
ABSTRACT We report three cases of tuberculosis in alpacas from Spain caused by Mycobacterium bovis . The animals revealed two different lesional patterns. Mycobacterial culture and PCR assay yielded positive results for M. Molecular typing the isolates identified spoligotype SB0295 identical variable-number tandem repeat (VNTR) allele sizes.
In vitro generated monocyte-derived dendritic cells (moDCs) have frequently been used to study the influence of porcine reproductive and respiratory syndrome virus (PRRSV) infection on antigen presenting cells. However, obtained results often conflicting in regard expression co-stimulatory molecules interaction with T this we performed a detailed phenotypic characterisation PRRSV-infected moDCs non-infected moDCs. For CD163 CD169, which are involved PRRSV-entry into host cells, our show that...
Rapid and accurate diagnostic tools, such as Real-Time PCR (qPCR), need to be implemented a confirmatory test in the framework of bovine tuberculosis (bTB) surveillance control programs, shortening turnaround time confirm bTB infection. The present study aimed evaluate direct qPCR from fresh tissue samples targeting insertion sequence IS 6110 using individually homogenized lymph nodes compared with microbiological culture. Retropharyngeal, tracheobronchial, mesenteric ( n = 687) were...
ObjectivesTo investigate the contribution to virulence of surface protein internalin B (InlB) in Listeria monocytogenes lineage I strain F2365, which caused a deadly listeriosis outbreak California 1985.MethodsThe F2365 displays point mutation that hampers expression InlB. We rescued InlB L. by introducing codon 34 (TAA CAA). investigated its importance for bacterial using vitro cell infection systems and murine intravenous model.ResultsIn HeLa JEG-3 cells, InlB+ expressing was ≈9-fold...
Tuberculosis in animals is caused by members of the
The diagnosis of bovine tuberculosis (bTB) is based on the single intradermal tuberculin test (SIT), interferon gamma, and compulsory slaughter reactor animals. Culture PCR from fresh tissue are regarded as gold standard techniques for post-mortem confirmation, with former being time-consuming presenting moderate to low sensitivity latter promising results. Histopathology has advantage identify categorize lesions in both non-reactor Therefore, this study aims highlight role histopathology...
Porcine reproductive and respiratory syndrome (PRRS) is considered one of the most important diseases in swine industry. Although several studies have been carried out to elucidate host immune response evoked against PRRS virus (PRRSV), there are aspects that still remain unclear. The aim this study was determine expression IL-1α, IL-6 TNF-α lymphoid organs (mediastinal retropharyngeal lymph nodes tonsil) PRRSV-infected pigs their correlation with PRRSV antigen. Proinflammatory cytokine...