A5064566554- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- Microbial Inactivation Methods
- Salmonella and Campylobacter epidemiology
- Microbial Metabolism and Applications
- Tuberculosis Research and Epidemiology
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Yersinia bacterium, plague, ectoparasites research
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- interferon and immune responses
- Bacterial Genetics and Biotechnology
- Bacillus and Francisella bacterial research
- Microbial Community Ecology and Physiology
- Vibrio bacteria research studies
- Vector-borne infectious diseases
- Antifungal resistance and susceptibility
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Biochemical and Molecular Research
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Peptides and Activities
- Clostridium difficile and Clostridium perfringens research
- CRISPR and Genetic Engineering
Institut Pasteur
2016-2025
Université Paris Cité
2016-2025
Centre National de la Recherche Scientifique
2021-2024
Sorbonne Paris Cité
2011-2021
Inserm
2009-2019
Délégation Paris 7
2011-2019
Laboratoire National de Référence
2019
Sorbonne Université
2019
Département Génétique Animale
2016
Institut National de la Recherche Agronomique du Niger
2015
Listeria monocytogenes is a food-borne pathogen with high mortality rate that has also emerged as paradigm for intracellular parasitism. We present and compare the genome sequences of L. (2,944,528 base pairs) nonpathogenic species, innocua (3,011,209 pairs). found large number predicted genes encoding surface secreted proteins, transporters, transcriptional regulators, consistent ability both species to adapt diverse environments. The presence 270 149 strain-specific (clustered in 100 63...
Listeria monocytogenes is a human intracellular pathogen that able to survive in the gastrointestinal environment and replicate macrophages, thus bypassing early innate immune defenses. Peptidoglycan (PG) an essential component of bacterial cell wall readily exposed host and, thus, important target for system. Characterization PG from L. demonstrated deacetylation N-acetylglucosamine residues. We identified N-deacetylase gene, pgdA, genome sequence. Inactivation pgdA revealed key role this...
Summary Listeria monocytogenes is a bacterial pathogen causing severe food‐borne infections in humans and animals. It can sense adapt to variety of harsh microenvironments outside as well inside the host. Once ingested by mammalian host, reaches intestinal lumen, where it encounters bile salts which, addition their role digestion, have antimicrobial activity. Comparison L. innocua genomes has revealed presence an ‐specific putative gene encoding salt hydrolase (BSH). Here, we show that bsh...
Summary Signature‐tagged mutagenesis (STM) was used to identify new genes involved in the virulence of Gram‐positive intracellular pathogen Listeria monocytogenes . One mutants isolated by this technique had transposon inserted virR, a gene encoding putative response regulator two‐component system. Deletion virR severely decreased mice as well invasion cell‐culture experiments. Using transcriptomic approach, we identified 12 regulated VirR, including dlt ‐operon, previously reported be...
L. monocytogenes is a facultative intracellular bacterium responsible for listeriosis. It able to invade, survive and replicate in phagocytic non-phagocytic cells. The infectious process at the cellular level has been extensively studied many virulence factors have identified. Yet, role of InlK, member internalin family specific monocytogenes, remains unknown. Here, we first show using deletion analysis vivo infection, that InlK bona fide factor, poorly expressed vitro well vivo, it anchored...
Listeria monocytogenes is responsible for gastroenteritis in healthy individuals and a severe invasive disease immunocompromised patients. Among the three identified L. evolutionary lineages, lineage I strains are overrepresented epidemic listeriosis outbreaks, but mechanisms underlying higher virulence potential of this remain elusive. Here, we demonstrate that Listeriolysin S (LLS), factor only present subset strains, bacteriocin highly expressed intestine orally infected mice alters host...
Microbial pathogens have evolved mechanisms to overcome immune responses and successfully infect their host. Here, we studied how Listeria monocytogenes evades detection by peptidoglycan (PGN) modification. By analyzing L. muropeptides, detected O-acetylated muramic acid residues. We identified an O-acetyltransferase gene, oatA, in the genome sequence. Comparison of PGN from parental isogenic oatA mutant strains showed that OatA O-acetylates PGN. also found O-acetylation confers resistance...
ISG15 is an interferon-stimulated, linear di-ubiquitin-like protein, with anti-viral activity. The role of during bacterial infection remains elusive. We show that expression in nonphagocytic cells dramatically induced upon Listeria infection. Surprisingly this induction can be type I interferon independent and depends on the cytosolic surveillance pathway, which senses DNA signals through STING, TBK1, IRF3 IRF7. Most importantly, we observed restricts vitro vivo. made use stable isotope...
Understanding the role of microbiota components in either preventing or favoring enteric infections is critical. Here, we report discovery a Listeria bacteriocin, Lmo2776, which limits intestinal colonization. Oral infection conventional mice with Δlmo2776 mutant leads to thinner mucus layer and higher loads both content deeper tissues compared WT Listeria. This latter difference dependent, as it not observed germ-free mice. Strikingly, phenocopied by pre-colonization before Prevotella...
Abstract ISG15 is an interferon-stimulated, ubiquitin-like protein, with anti-viral and anti-bacterial activity. Here, we map the endogenous in vivo ISGylome liver following Listeria monocytogenes infection by combining murine models of reduced or enhanced ISGylation quantitative proteomics. Our method identifies 930 sites 434 proteins also detects changes host ubiquitylome. The ISGylated targets are enriched which alter cellular metabolic processes, including upstream modulators catabolic...
Summary Listeria monocytogenes is an opportunistic food‐borne human and animal pathogen. Several surface proteins expressed by this intracellular pathogen are critical for the infectious process. By in silico analysis we compared protein repertories of L. non‐pathogenic species innocua identified a gene encoding absent . This that named aut encodes (Auto) 572 amino acids containing signal sequence, N‐terminal autolysin domain C‐terminal cell wall‐anchoring made up four GW modules. We show...
Staphylococcus aureus is a common human cutaneous and nasal commensal major life-threatening pathogen. Adaptation to the different environments encountered inside outside host crucial requirement for survival colonization. We identified characterized eukaryotic-like serine/threonine kinase with three predicted extracellular PASTA domains (SA1063, or Stk1) its associated phosphatase (SA1062, Stp1) in S. aureus. Biochemical analyses revealed that Stk1 displays autokinase activity on threonine...
Listeria monocytogenes is an intracellular pathogen responsible for severe foodborne infections. It can replicate in both phagocytic and nonphagocytic mammalian cells. The infectious process at the cellular level has been studied extensively, but how bacterium overcomes early host innate immune responses remains largely unknown. Here we show that InlC, a member of internalin family, secreted intracellularly directly interacts with IKKα, subunit IκB kinase complex critical phosphorylation...
ABSTRACT Aspergillus fumigatus is the main cause of invasive aspergillosis in immunocompromised patients, and only a limited number drugs for treatment are available. A screening method new antifungal compounds urgently required, preferably an approach suitable vitro vivo studies. Bioluminescence imaging powerful tool to study temporal spatial resolutions infection effectiveness drugs. Here, we describe construction bioluminescent A. strain by fusing promoter glyceraldehyde-3-phosphate...
The mycobacterial IdeR protein is a homologue of the diphtheria‐toxin repressor DtxR. We have previously demonstrated that Mycobacterium tuberculosis IdeR, like DtxR, represses transcription Corynebacterium diphtheriae iron‐regulated promoters in vivo and binds to C. operators metal‐dependent manner vitro . show here ideR mutants M. smegmatis , constructed by allelic replacement, were defective their ability repress siderophore biosynthesis presence iron. They also more sensitive hydrogen...