A5042818837- Bacterial Infections and Vaccines
- RNA and protein synthesis mechanisms
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Listeria monocytogenes in Food Safety
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- CRISPR and Genetic Engineering
- Antimicrobial Peptides and Activities
- Respiratory viral infections research
- Bacterial biofilms and quorum sensing
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Enterobacteriaceae and Cronobacter Research
- Glycosylation and Glycoproteins Research
- Molecular Biology Techniques and Applications
- Escherichia coli research studies
- Hormonal Regulation and Hypertension
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Advanced Proteomics Techniques and Applications
- Cardiac Imaging and Diagnostics
- Transplantation: Methods and Outcomes
- Antimicrobial Resistance in Staphylococcus
Karolinska Institutet
2018-2025
Karolinska University Hospital
2019-2024
Nanyang Technological University
2018-2023
Singapore Centre for Environmental Life Sciences Engineering
2018-2023
Mbarara University of Science and Technology
2020
Nanyang Polytechnic
2019
University of Oxford
2013-2016
Umeå University
2006-2011
UCLouvain Saint-Louis Brussels
1995
Neisseria meningitidis causes bacterial meningitis and septicemia. It evades the host complement system by upregulating expression of immune evasion factors in response to changes temperature. RNA thermometers within mRNAs control factors, including CssA, 5′-untranslated region operon for capsule biosynthesis. We dissect molecular mechanisms thermoregulation report structure CssA thermometer. show that thermometer acts as a rheostat, whose stability is optimized respond small temperature...
During colonisation of the upper respiratory tract, bacteria are exposed to gradients temperatures. Neisseria meningitidis is often present in nasopharynx healthy individuals, yet can occasionally cause severe disseminated disease. The meningococcus evade human complement system using a range strategies that include recruitment negative regulator, factor H (CFH) via binding protein (fHbp). We have shown previously fHbp levels influenced by ambient temperature, with more produced at higher...
Nanoparticles exhibit potential as drug carriers in biomedicine due to their high surface-to-volume ratio that allows for facile loading. Nanosized delivery systems have been proposed the of biologics facilitating transport across epithelial layers and maintaining stability against proteolytic degradation. Here, we capitalize on a nanomanufacturing process famous its scalability reproducibility, flame spray pyrolysis, produce calcium phosphate (CaP) nanoparticles with tailored properties....
Abstract Protein glycosylation is increasingly recognized as a common protein modification across bacterial species. Within the Neisseria genus O ‐linked conserved yet closely related species express O‐ oligosaccharyltransferases (PglOs) with distinct targeting activities. this work, we explore capacity of different PglOs using Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) fractionation and Data‐Independent Acquisition (DIA) to allow characterization impact changes in on...
ABSTRACT Degradation of immunoglobulin (Ig) represents an important bacterial immune evasion strategy. For mucosal colonization, degradation IgA is particular importance, and many bacteria secrete specific proteases that typically target the extended hinge region IgA1. Such a specialized protease has not yet been reported in Group A Streptococcus (GAS), despite its ability to successfully colonize human surfaces. In this study, we focused on cysteine SpeB secreted by GAS analyzed interaction...
Expression of virulence factors in the human bacterial pathogen Listeria monocytogenes is almost exclusively regulated by transcriptional activator PrfA. The translation prfA controlled a thermosensor located 5'-untranslated RNA (UTR), and high at 37°C low temperatures <30°C. In order to develop thermoregulated translational expression system, 5'-UTR different lengths prfA-coding sequences were placed front lacZ. When expressed Escherichia coli, β-galactosidase was directly correlated length...
Aspergillus fumigatus is an ubiquitous, filamentous and opportunistic pathogenic fungus which causes fatal invasive aspergillosis among immuno-compromised patients. Since therapeutic strategies are currently limited, the mortality rate of high thus, alternative antifungal required. In this study, we demonstrate that during vegetative growth able to scavenge nucleic acids within its cell wall with accumulation rates several thousand-fold, compared surrounding medium. To investigate, whether...
Molecular mechanisms by which sex steroids interact with P. aeruginosa to modulate its virulence have yet be reported. Our work provides the first characterization of a steroid-induced membrane stress mechanism promoting virulence, includes release proinflammatory outer vesicles, resulting in inflammation, host tissue damage, and reduced bacterial clearance. We further demonstrate that at nanomolar (physiological) concentrations, male female promote clinical strains based on their dynamic...
ABSTRACT Expression of type four pili (Tfp) is essential for virulence in Neisseria meningitidis . Pili mediate adhesion, bacterial aggregation, and DNA uptake. In N. , the major pilin subunit encoded by pilE gene. some strains, PilE subject to phase antigenic variation, which can alter Tfp properties together offer a possible mechanism immune escape. Pilin expression variation be modulated response environmental cues; however, precise mechanisms such regulation remain unclear. We identified...
Circulating levels of glucocorticoids show a circadian rhythm. Obesity is associated with flattening the diurnal rhythm; plasma cortisol are slightly increased during trough, although they normal or low in morning. Studies humans and leptin-resistant Zucker rats suggest that tissue-specific alterations glucocorticoid exposure might play key role for development obesity obesity-associated dysregulation hypothalamic-pituitary-adrenal axis. We hypothesized there rhythm prereceptor metabolism...
Neisseria meningitidis is the causative agent of invasive meningococcal disease and polysaccharide capsule one its major virulence factors. Biosynthesis controlled by an RNA thermosensor (RNAT) in 5'-untranslated region (5'-UTR) cssA gene. The function RNAT depends on 8-bp tandem repeat configuration. We aimed to identify characterise novel RNATs isolates responsible for regulating production.We investigated allele igr_up_NEIS0055, containing 5'-UTR gene, clinical which whole-genome...
Bacterial meningitis is a major cause of death and disability in children worldwide. Two human restricted respiratory pathogens, Streptococcus pneumoniae Haemophilus influenzae , are the causative agents bacterial meningitis, attributing to 200,000 deaths annually. These pathogens often part nasopharyngeal microflora healthy carriers. However, what factors elicit them disseminate invasive diseases, remain unknown. Elevated temperature fever hallmarks inflammation triggered by infections can...
Neisseria meningitidis (meningococcus) is a common bacterial colonizer of the human nasopharynx but can occasionally cause very severe systemic infections with rapid onset. Meningococci are able to degrade IgA encountered during colonization mucosal membranes using their IgA1-specific serine protease. During infection, specific IgG induce complement-mediated lysis bacterium. However, meningococcal immune evasion mechanisms in thwarting remain undescribed. In this study, we report for first...
Increasing evidence has demonstrated that regulatory RNA elements such as riboswitches (RS) play a pivotal role in the fine-tuning of bacterial gene expression. In this study, we investigated and characterized novel transcriptional thiamine pyrophosphate (TPP) RS obligate human pathogen N. meningitidis MC58 (serogroup B). This is located 5´ untranslated region upstream thiC gene, encoding protein involved TPP biosynthesis, an essential cofactor for all living beings. Primer extension...
Background A timely differential diagnostic is essential to identify the etiology of central nervous system (CNS) infections in children, order facilitate targeted treatment, manage patients, and improve clinical outcome. Objective The Pediatric Infection-Point-of-Care (PI-POC) trial investigating novel methods strengthen diagnostics suspected childhood CNS low-income health systems such as those Southwestern Uganda. This will be achieved by evaluating (1) a DNA-based assay for infections,...