Effective monitoring of microbial pathogens is essential for a successful preventive food safety and hygiene strategy. However, as most strategies are growth-based, these tests fail to detect pathogenic bacteria that have entered the viable but non-culturable (VBNC) state. The present study reports induction VBNC state in five human by commercially available household cleaners combination with inorganic salts. We determined non-ionic surfactants, common ingredient cleaners, can induce state,...

Abstract A reduction in animal-based diets has driven market demand for alternative meat products, currently raising a new generation of plant-based alternatives (PBMAs). It remains unclear whether these substitutes are short-lived trend or become established the long term. Over last few years, increasing sales and diversifying product range continued, but publication activities this field limited mainly to research food technology topics. As their popularity increases, questions emerge...

The droplet digital polymerase chain reaction (ddPCR) determines DNA amounts based upon the pattern of positive and negative droplets, according to Poisson distribution, without use external standards. However, division into droplets is often not clear because a part has intermediate fluorescence values, appearing as "rain" in plot. Despite rain, absolute quantification with ddPCR possible, shown previously for prfA assay quantifying Listeria monocytogenes. Nevertheless, reducing thus...

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 396:211-219 (2009) - DOI: https://doi.org/10.3354/meps08281 Genetic population structures of blue starfish Linckia laevigata and its gastropod ectoparasite Thyca crystallina M. Kochzius1,*,**, C. Seidel1,2, J. Hauschild1,3, S. Kirchhoff1, P. Mester1, I. Meyer-Wachsmuth1, A. Nuryanto1,4, Timm1 1Biotechnology...

Fast and reliable pathogen detection is an important issue for human health. Since conventional microbiological methods are rather slow, there growing interest in quantification using molecular methods. The droplet digital polymerase chain reaction (ddPCR) a relatively new PCR method absolute accurate without external standards. Using the Listeria monocytogenes specific prfA assay, we focused on questions of whether assay was directly transferable to ddPCR suitable samples derived from...

An increasing number of publications describe the potential ionic liquids (ILs) as novel antimicrobials, antibacterial coatings and even active pharmaceutical ingredients. Nevertheless, a major research area, notably their impact on viruses, has so far been neglected. Consequently aim this study was to examine effects ILs infectivity viruses. A systematic analysis investigate defined structural elements virus activity performed using 55 ILs. All structure relationships (SARs) were tested...

For three decades now, ionic liquids (ILs), organic salts comprising only ions, have emerged as a new class of pharmaceuticals. Although recognition the antimicrobial effects ILs is growing rapidly, there almost nothing known about their possible virucidal activities. This probably reflects paucity understanding virus inactivation. In this study, we performed systematic analysis to determine effect specific structural motifs on different biological test systems (viruses, bacteria and...

FTIR spectroscopic analysis of two IL species with similar high antimicrobial activity ([TC₈MA]/[TMC₁₆A]) revealed independently their anion different modes action against <italic>S. typhimurium</italic> through alterations in the bacterial membrane fluidity.

Many bacteria enter the viable but non-culturable (VBNC) state to maximize resources and increase their tolerance harmful conditions cope with environmental stress, which has been described for a plethora of important human foodborne pathogens. VBNC pathogens can potentially present serious risk health as they are invisible routine microbiological culture-based methods. Of high importance is increased antibiotics or disinfectant measures while in state. The greatest remaining challenge such...

Fatty acid-based ionic liquids (FAILs) are an emerging class of biodegradable ILs. Although long-chained FAILs in particular offer a promising alternative to problematic hydrophobic ILs lubrication and extraction applications, recent studies have revealed the possibility anionic "side-chain length effect", which would adversely impact their eco-friendliness. This study therefore investigated toxicity with side-chain lengths between C1 C18 benign cations by applying tripartite biological test...

DNA is one of the most frequently analyzed molecules in life sciences. In this article we describe a simple and fast protocol for quantitative isolation from bacteria based on hydrophobic ionic liquid supported cell lysis at elevated temperatures (120–150 °C) subsequent PCR-based analysis. From set five liquids, 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide was identified as suitable extraction because limited PCR inhibition by aqueous eluate well no detectable uptake. The...

A rapid real-time PCR-based method for the detection of Listeria monocytogenes was applied to examination 44 Quargel cheese samples from a recent outbreak in Austria and compared standard according ISO-16140.The combined enrichment/real-time PCR amplifying prfA locus performed [Rossmanith et al.(2006) Res Microbiol, 157, 763-771]. Qualitative quantitative ISO-11290. Comparison with ISO-11290 resulted 100% relative accuracy, sensitivity specificity.A previously published study describing...

Over the past decades, ionic liquids (ILs) have gained considerable attention from scientific community because of their versatile and designable properties. As a result, there are numerous IL applications, not only in organic synthesis, catalysis, or extraction but also as active pharmaceutical ingredients novel antimicrobials. While effort has been put into developing quantitative structure–activity relationship (QSAR) models for toxicity prediction, little is known about actual mode...