A5015952810- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Microbial infections and disease research
- Salmonella and Campylobacter epidemiology
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Veterinary medicine and infectious diseases
- Gut microbiota and health
- Vector-Borne Animal Diseases
- Dermatology and Skin Diseases
- Animal health and immunology
- Bacillus and Francisella bacterial research
- Monoclonal and Polyclonal Antibodies Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Agricultural Systems and Practices
- Agricultural economics and policies
- Mycobacterium research and diagnosis
- Herpesvirus Infections and Treatments
- Zoonotic diseases and public health
- Reproductive Physiology in Livestock
- Poxvirus research and outbreaks
- Animal Behavior and Welfare Studies
- Infectious Diseases and Mycology
- Viral Infections and Immunology Research
Austrian Agency for Health and Food Safety
2022-2024
University of Veterinary Medicine Vienna
2016-2023
Gesundheit Österreich
2022
International Atomic Energy Agency
2020-2021
University of Parma
2014
In the literature, there is absent or weak evidence on effectiveness of biosecurity measures to control Salmonella spp. and hepatitis E virus (HEV) pig farms. Therefore, present study aimed collect, weigh, compare opinions from experts relevance several measures. An online questionnaire was submitted selected experts, multiple European countries, knowledgeable either HEV spp., in indoor outdoor farming systems (settings). The ranked eight categories with regards reducing two pathogens...
Porcine contagious pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) remains one of the major causes poor growth performance and respiratory disease in pig herds. While role antibodies against APP has been intensely studied, porcine T cell response poorly characterized. To address this, pigs were intranasally infected with serotype 2 euthanized during acute phase [6–10 days post-infection (dpi)] or chronic infection (27–31 dpi). Lymphocytes isolated from blood, tonsils, lung...
Reproductive disorders induced by porcine reproductive and respiratory syndrome virus (PRRSV) cause high economic losses in the pig industry worldwide. In this study, we aimed to phenotypically characterize a virulent PRRSV-1 subtype 1 isolate (AUT15-33) model. Furthermore, protective effect of heterologous modified live vaccine (ReproCyc® PRRS EU) was evaluated. addition, PRRSV AUT15-33 genotypically compared other well-characterized isolates. Sixteen gilts were equally divided into four...
Actinobacillus (A.) pleuropneumoniae is the causative agent of porcine pleuropneumonia and causes significant losses in pig industry worldwide. Early host immune response crucial for further progression disease. A. either rapidly eliminated by system or switches to a long-term persistent form. To gain insight into host-pathogen interaction during early stages infection, pigs were inoculated intratracheally with serotype 2 humanely euthanized eight hours after infection. Gene expression...
The phenotype and function of immune cells that reside at the maternal-fetal interface in humans mice have been, still are, extensively studied with aim to fully comprehend complex immunology pregnancy. In pigs, information regarding cell phenotypes is limited mainly focused on early gestation whereas late has not yet been investigated. We designed a unique methodology tailored porcine epitheliochorial placenta, which allowed us address separately maternal endometrium (ME) fetal placenta...
The gram-negative facultative intracellular bacteria Salmonella Typhimurium (STM) often leads to subclinical infections in pigs, but can also cause severe enterocolitis this species. Due its high zoonotic potential, the pathogen is likewise dangerous for humans. Vaccination with a live attenuated STM strain (Salmoporc) regarded as an effective method control affected pig herds. However, information on cellular immune response of swine against still scarce. In study, we investigated T-cell...
The novel pestivirus species known as lateral-shaking inducing neuro-degenerative agent (LINDA) virus emerged in 2015 a piglet-producing farm Austria. Affected piglets showed strong congenital tremor result of severe lesions the central nervous system. Here, we report results controlled animal infection experiment. Post-weaning were infected with LINDA to determine susceptibility pigs, clinical consequences and humoral immune response against LINDA. No clinically overt disease signs observed...
Hepatitis E virus (HEV) genotype 3 is a prevalent zoonotic pathogen in European pig farms, posing significant public health risk primarily through the foodborne route. The study aimed to identify effective biosecurity measures for controlling HEV transmission on addressing critical gap current knowledge. Utilizing cross-sectional design, fecal samples from gilts, dry sows, and fatteners were collected 231 farms of all farm types across nine countries. Real-time RT-PCR was employed test these...
Raising of alpacas as exotic livestock for wool and meat production companion animals is growing in importance the United States, Europe Australia. Furthermore alpaca, well rest camelids, possesses peculiarity producing single-chain antibodies from which nanobodies can be generated. Nanobodies, due to their structural simplicity reduced size, are very versatile terms manipulation bio-therapeutic exploitation. In fact biotech companies involved nanobody application continue grow number size....
Weaning often leaves the piglet vulnerable to gut dysfunction. Little is known about acute response of a mucosa primed by milk-oriented microbiome before weaning plant-oriented (POM) after weaning. We evaluated epithelial structure, secretory and permeability in small large intestines piglets receiving milk-based (i.e., preweaning) or plant-based diet postweaning) POM inocula using intestinal loop perfusion assays (ILPA). The were prepared from jejunal colonic digesta four 7 week-old weaned...
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most devastating viruses for global swine industry. Infection during late gestation causes failure but local immune response in utero remains poorly understood. In this study, an experimental PRRSV-infection model with two different PRRSV-1 field isolates was used to investigate cell phenotypes at maternal-fetal interface gestation. addition, phenotypic changes induced by a modified live (MLV, ReproCyc ® PRRS EU)...
Sheeppox (SPP) is a highly contagious disease of small ruminants caused by sheeppox virus (SPPV) and predominantly occurs in Asia Africa with significant economic losses. SPPV genetically immunologically closely related to goatpox (GTPV) lumpy skin (LSDV), which infect goats cattle respectively. live attenuated vaccines (LAVs) are used for vaccination against SPP (GTP). Mechanisms innate immunity elicited unknown. Although adaptive responsible long-term immunity, it the responses that...
In high-income countries, the hepatitis E virus (HEV) is considered an emerging threat causing autochthonous acute in humans, with increased number of reported cases over last 10 years and related burden chronic immunocompromised transplant patients. Pigs are main reservoir HEV-3 genotype, which most common Europe, can be transmitted to humans through consumption raw undercooked pork products. Extensive sequencing revealed existence several subtypes both pigs, confirming a broad...
Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae affects pig health status and the swine industry worldwide. Despite extensive number of studies focused on A. infection vaccine development, a thorough analysis exoproteome is still missing. Using complementary approach quantitative proteomics immunoproteomics we gained an in-depth insight into serotype 2 exoproteome, which provides basis for future functional studies. Label-free liquid chromatography-tandem mass spectrometry...
As part of the One Health European Joint Programme, project “Biosecurity practices for pig farming across Europe” (BIOPIGEE) aims to establish an inventory relevant biosecurity measures that reflects their effectiveness in reducing occurrence Salmonella spp. and hepatitis E virus (HEV) production. While term “biosecurity” is well defined, a harmonized definition ”biosecurity measure” (BSM) missing. In consequence, there lack clarity, understanding acceptence, on which can be considered BSMs....
Salmonella spp. and hepatitis E virus (HEV) are significant foodborne zoonotic pathogens that impact the health of livestock, farmers, general public. This study aimed to identify biosecurity measures (BSMs) against these on swine farms in Europe, United States, Canada. Overall, 1,529 articles from three scientific databases were screened manually with artificial intelligence (AI) tool ASReview. We identified 54 BSMs 32 articles, primarily focused control. Amongst extracted BSMs, only five...
<title>Abstract</title> Background: In the literature, there is absent or weak evidence on effectiveness of biosecurity measures to control of<italic>Salmonella</italic>spp. and hepatitis E virus (HEV) pig farms. Therefore, present study aimed collect, weigh, compare opinions from experts relevance several measures. An online questionnaire was submitted selected experts, multiple European countries, knowledgeable either HEV or<italic>Salmonella</italic>, in indoor outdoor farming systems...
Abstract In the immediate time after weaning, piglets often show symptoms of gut inflammation. The change to a plant-based diet, lack sow milk, and resulting novel microbiome metabolite profile in digesta may be causative factors for observed We used intestinal loop perfusion assay (ILPA) investigate jejunal colonic expression genes antimicrobial secretion, oxidative stress, barrier function, inflammatory signaling suckling weaned when exposed “plant-oriented” (POM) representing postweaning...