Giuseppe Aprea
A5016101295- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Hepatitis Viruses Studies and Epidemiology
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Veterinary medicine and infectious diseases
- Biosensors and Analytical Detection
- Listeria monocytogenes in Food Safety
- Aquaculture disease management and microbiota
- Protein Hydrolysis and Bioactive Peptides
- Gut microbiota and health
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- Biopolymer Synthesis and Applications
- Microbial Inactivation Methods
- Tea Polyphenols and Effects
- Respiratory viral infections research
- Digestive system and related health
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Toxoplasma gondii Research Studies
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2015-2025
Ege University
2023
Istituto Zooprofilattico Sperimentale del Mezzogiorno
2010-2017
This study adds insight regarding the occurrence and spread of SARS-CoV-2 Variants Concern (VOCs) Interest (VOIs) in Italy October November 2022, by testing urban wastewater collected throughout country. A total 332 samples were from 20 Italian Regions/Autonomous Provinces (APs) within framework national environmental surveillance. Of these, 164 first week 168 November. ∼1600 bp fragment spike protein was sequenced Sanger (for individual samples) long-read nanopore sequencing pooled...
Campylobacter has developed resistance to several antimicrobial agents over the years, including macrolides, quinolones and fluoroquinolones, becoming a significant public health hazard. A total of 145 strains derived from raw milk, chicken faeces, carcasses, cattle faeces human collected various Italian regions, were screened for susceptibility, molecular characterization (SmaI pulsed-field gel electrophoresis) detection virulence genes (sequencing DNA microarray analysis). The prevalence...
Listeriosis, caused by Listeria monocytogenes , is a severe foodborne infection, particularly hazardous for pregnant women and immunocompromised individuals. In Europe, L. was the fifth most reported zoonotic agent in 2022, with outbreaks frequently linked to ready‐to‐eat (RTE) foods. Addressing food contamination innovative antimicrobial strategies critical enhancing safety reducing public health risks. This study evaluated efficacy of two bacteriophage‐derived endolysins, LP101_021...
This study aimed to analyse the diversity of Campylobacter jejuni population in broilers and evaluate major source contamination poultry meat. Eight rearing cycles over one year provided samples from three different broiler farms processed at same slaughterhouse. A total 707 C. were isolated cloacal swabs before slaughter breast skin carcasses after chilling. All suspected colonies identified with PCR assays was genotyped by sequence analysis flaA short variable region (SVR) pulsed-field gel...
Hepatitis E virus (HEV) is a zoonotic pathogen with worldwide distribution, and infects several mammalian species, including pigs wild boars, which are recognized as its natural reservoirs. The causes usually self-limiting liver disease mortality rate generally below 1%, although rates of 15%–25% have been recorded in pregnant woman. Chronic infections can also occur. prevalence HEV has extensively studied boars northern Italy, where intensive pig herds predominantly located. In contrast,...
Campylobacteriosis is the most commonly reported gastrointestinal disease in humans. Campybacter jejuni main cause of infection, and bacterial colonization broiler chickens widespread difficult to prevent, leading high risk occurrence meat. Phage therapy represents an alternative strategy control Campylobacter poultry. The aim this work was assess efficacy two field-isolated bacteriophages against experimental infections with anti-microbial resistant (AMR) strain. A two-step phage...
The emergence of new SARS-CoV-2 variants poses challenges to global surveillance efforts, necessitating swift actions in their detection, evaluation, and management. Among the most recent variants, Omicron BA.2.86 its sub-lineages have gained attention due potential immune evasion properties. This study describes development a digital PCR assay for rapid detection descendant lineages, wastewater samples. By using this assay, we analyzed samples collected Italy from September 2023 January...
Bacteriophages or more commonly "phages" are bacterial viruses.They ubiquitous and good indicators of contaminations since their prevalence is high in those environments where hosts abundant.Phage classification based on morphology for this reason, even though it considered an old technique, TEM (Transmission Electron Microscopy) still plays a key role characterization.In the present work, authors focused analysis phage ɸApr-1 isolated against Lactococcus lactis (L.lactis), implicated...
This literature review was aimed to collect investigations on the in vivo evidences for bacteria associated fermented dairy foods behave as probiotics with beneficial effects prevention and treatment of various diseases. All main bacterial groups commonly present high numbers milks or cheeses were taken into account, namely starter lactic acid (SLAB) Lactobacillus delbrueckii subsp. bulgaricus lactis, L. helveticus, Lactococcus Strepto-coccus thermophilus, non LAB (NSLAB) Lacticaseibacillus...
Hepatitis E virus (HEV) is an emergent zoonotic pathogen, causing worldwide acute and chronic hepatitis in humans. HEV comprises eight genotypes several subtypes. 3 4 (HEV3 HEV4) are zoonotic. In Italy, the most part of infections (80%) due to autochthonous HEV3 circulation virus, key role played by wild animals generally accepted. Abruzzo Italian region officially considered "hot spot", with subtype HEV3-c being up now only one reported among boars. During year 2018-2019, a group boars were...
Foodborne transmission is considered the main way of spreading zoonotic hepatitis E virus (HEV) infection in Europe. In recent years, human cases subjects without history travel endemic areas have raised, suggesting that domestic HEV increasing. Pork products with or liver, are often indicated as source many foodborne well small outbreaks. Pigs recognized reservoir HEV-3 genotype, most frequently detected EU. absence a harmonized surveillance circulation, data on prevalence heterogeneous but...
Abstract Background Lytic bacteriophages that infect Campylobacter spp. have been utilized to develop therapeutic/decontamination techniques. However, the association of and has focus several strands research aimed at understanding complex relationships developed between predators prey over evolutionary time. The activities endogenous temperate used evaluate genomic rearrangements differential protein expression in host cells, mechanisms resistance bacteriophage infection campylobacters such...
Listeria monocytogenes is a bacterial pathogen responsible of listeriosis, disease that in humans often related to the contamination ready-to-eat foods. Phages are candidate biodecontaminants pathogenic bacteria thanks their ability lyse prokaryotes while being safe for eukaryotic cells. In this study, ΦIZSAM-1 was isolated from drain-waters an Italian blue cheese plant and showed lytic activity against antimicrobial resistant strains. This phage subjected purification vitro efficacy tests....
Hepatitis E virus (HEV) is a zoonotic pathogen, causing infectious hepatitis in man. Pigs and wild boars are the natural asymptomatic reservoirs, while disease humans could be either or evolve hepatitis. In Europe, an increasing number of human infections from HEV have been reported over last few years. The main route transmission through contaminated food, by direct indirect consumption raw undercooked pork boar meat products. Up to now, prevalence Italian northern regions has extensively...
spp. is the second most common bacteria associated with foodborne gastrointestinal outbreaks in humans, highest contamination levels meat, especially poultry.
Wastewater-based epidemiology (WBE) is a recognized, dynamic approach to monitoring the transmission of pathogens in communities through urban wastewater. This study aimed detect and quantify influenza A B viruses Italian wastewater during 2022–2023 season (October 2022 April 2023). total 298 samples were collected from 67 treatment plants (WTPs) across country. These analyzed for (IAV, IBV) using primers originally developed by Centers Disease Control Prevention (CDC) real-time PCR adapted...
Salmonella spp. and hepatitis E virus (HEV) are significant foodborne zoonotic pathogens that impact the health of livestock, farmers, general public. This study aimed to identify biosecurity measures (BSMs) against these on swine farms in Europe, United States, Canada. Overall, 1,529 articles from three scientific databases were screened manually with artificial intelligence (AI) tool ASReview. We identified 54 BSMs 32 articles, primarily focused control. Amongst extracted BSMs, only five...