A5005432513- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Respiratory viral infections research
- Bacterial Identification and Susceptibility Testing
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Biopolymer Synthesis and Applications
- Protein Hydrolysis and Bioactive Peptides
- SARS-CoV-2 and COVID-19 Research
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Listeria monocytogenes in Food Safety
- Infections and bacterial resistance
- Yersinia bacterium, plague, ectoparasites research
- Biomedical and Chemical Research
- Vibrio bacteria research studies
- Botulinum Toxin and Related Neurological Disorders
- Aquaculture disease management and microbiota
- Veterinary medicine and infectious diseases
- Clostridium difficile and Clostridium perfringens research
Istituto Superiore di Sanità
2010-2024
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2017-2019
Sapienza University of Rome
2008-2010
Policlinico Umberto I
2010
Middle East respiratory syndrome coronavirus (MERS-CoV), which belongs to beta group of coronavirus, can infect multiple host species and causes severe diseases in humans. Multiple surveillance phylogenetic studies suggest a bat origin. In this study, we describe the detection full genome characterization two CoVs closely related MERS-CoV from Italian bats, Pipistrellus kuhlii Hypsugo savii. Pool viscera were tested by pan-coronavirus RT-PCR. Virus isolation was attempted inoculation...
Hepatitis E virus (HEV) is a zoonotic pathogen with worldwide distribution, and infects several mammalian species, including pigs wild boars, which are recognized as its natural reservoirs. The causes usually self-limiting liver disease mortality rate generally below 1%, although rates of 15%–25% have been recorded in pregnant woman. Chronic infections can also occur. prevalence HEV has extensively studied boars northern Italy, where intensive pig herds predominantly located. In contrast,...
Campylobacteriosis is the most commonly reported gastrointestinal disease in humans. Campybacter jejuni main cause of infection, and bacterial colonization broiler chickens widespread difficult to prevent, leading high risk occurrence meat. Phage therapy represents an alternative strategy control Campylobacter poultry. The aim this work was assess efficacy two field-isolated bacteriophages against experimental infections with anti-microbial resistant (AMR) strain. A two-step phage...
Abstract The aim of the study was to evaluate mRNA expression four relevant ABC‐transporter genes [MDR1 (P‐glycoprotein; Pgp), MRP1, MRP4, and MRP5] in HIV‐positive individuals failing treatment analyze association between levels their viral load, CD4 cell count, therapeutic history. Ninety‐eight samples 20 from healthy donors were analyzed, retrospectively. Peripheral blood mononuclear cells (PBMCs) HIV1‐positive collected at time virological failure. Expression Pgp, MRP5 PBMCs evaluated by...
Highly pathogenic (HP) and low (LP) avian influenza viruses (AIVs) belonging to H5 H7 subtypes have been found be associated with human infection as the result of direct transmission from infected poultry. Human infections by AIVs can cause mild or subclinical disease, serosurveys are believed represent an important tool identify risk zoonotic transmission. Therefore, we sought examine Italian poultry workers exposed during LPAI HPAI outbreaks aim assessing serologic evidence AIVs. From...
Background Pigs play a key epidemiologic role in the ecology of influenza A viruses (IAVs) emerging from animal hosts and transmitted to humans. Between 2008 2010, we investigated health risk occupational exposure swine (SIVs) Italy, during emergence spread 2009 H1N1 pandemic (H1N1pdm) virus. Methodology/Principal Findings Serum samples 123 workers (SWs) 379 control subjects (Cs), not exposed pig herds, were tested by haemagglutination inhibition (HI) assay against selected SIVs belonging...
Ecological interactions between wild aquatic birds and outdoor-housed poultry can enhance spillover events of avian influenza viruses (AIVs) from reservoirs to domestic birds, thus increasing the related zoonotic risk occupationally exposed workers. To assess serological evidence AIV infection in workers operating Northern Italy at wildfowl/poultry interface or directly wildfowl, serum samples were collected April 2005 November 2006 57 bird-exposed (BEWs) 7 unexposed controls (Cs), planning...
Enteroinvasive Escherichia coli (EIEC) cause intestinal illness through the same pathogenic mechanism used by Shigella spp. The latter species can be typed genomic and phenotypic methods for E. have been proposed reclassification within species. Recently first appearance of a high EIEC O96:H19 was described in Europe as causative agent two large outbreaks that occurred Italy United Kingdom. In contrast to spp majority strains, fermented lactose, lacked pathoadaptive mutations showed good...
Listeria monocytogenes is a bacterial pathogen responsible of listeriosis, disease that in humans often related to the contamination ready-to-eat foods. Phages are candidate biodecontaminants pathogenic bacteria thanks their ability lyse prokaryotes while being safe for eukaryotic cells. In this study, ΦIZSAM-1 was isolated from drain-waters an Italian blue cheese plant and showed lytic activity against antimicrobial resistant strains. This phage subjected purification vitro efficacy tests....
Swine influenza viruses (SIVs) have been repeatedly demonstrated to circulate in wild boar ( Sus scrofa) populations, whereas no evidence of exposure avian (AIVs) has described boar. To better understand how different environments may influence the ecology A (IAVs) suid we examined biologic samples boars from two study areas represented by an upland (UL) and a wetland (WL) northern central Italy, respectively. Serum were collected 388 sampled UL, both serum sample nasal swab obtained each 35...
It is necessary to understand the molecular nature of virus population that persists in cellular reservoirs. To achieve this we planned characterize patterns resistance HIV-1 CD14+ monocytes, CD4+ T cells, and plasma. Blood samples were collected from 42 patients treated for HIV: 32 virological failure 10 viremia was undetectable. cells isolated using magnetic beads. Genotyping reverse transcriptase protease gene undertaken fluorescent dideoxy-terminator method. Of failure, 24 (75%) had...
Following the emergence of A(H1N1)pdm09 in humans, this novel influenza virus was reverse transmitted from infected people to swine population worldwide. In study we investigated molecular evolution identified pigs reared a single herd. Nasal swabs taken showing respiratory distress were tested for type A and by real-time RT-PCR assays. Virus isolation positive samples attempted inoculation nasal into specific pathogen free embryonated chicken eggs (ECE) complete genome sequencing performed...
Coronaviruses (CoVs), a subfamily of Orthocoronavirinae, are viruses that sometimes present zoonotic character. Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is responsible for the recent outbreak COVID-19, which, since its in 2019, has caused about 774,593,066 confirmed cases and 7,028,881 deaths. Aereosols main route transmission among people; however, viral droplets can contaminate surfaces fomites as well particulate matter (PM) suspensions natural human origin. Honey bees...
This study focused on the characterization of lactic acid bacteria (LAB) in a renowned traditional Italian cheese, Pecorino di Farindola, order to select an autochthonous culture and investigate its potential for improvement safety functional properties. Two hundred six LAB isolated throughout production maturation from nine cheese lots three farms were identified by 16S rRNA gene sequencing tested presence genes encoding virulence factors, vancomycin resistance (for enterococci), biogenic...
This study focused on the characterization of lactic acid bacteria (LAB) in a renowned traditional Italian cheese, Pecorino di Farindola, to select an autochthonous culture and investigate its po-tential for improvement safety functional properties. Two hundred six LAB isolated throughout production maturation from nine cheese lots three farms were identified by 16S rRNA gene sequencing tested presence genes encoding virulence factors, vancomycin resistance (for enterococci), biogenic amines...
Free-living amoebae (FLA) are widely distributed protozoa in nature, known to cause severe eye infections and central nervous system disorders. There is growing attention the potential role that these could act as reservoirs of pathogenic bacteria and, consequently, possibility that, persistence spread latter may be facilitated, by exploiting internalization into amoebae. Shiga toxin-producing strains Escherichia coli (STEC) zoonotic agents capable causing serious diseases, such hemorrhagic...