A5071513520- SARS-CoV-2 detection and testing
- Marine Toxins and Detection Methods
- SARS-CoV-2 and COVID-19 Research
- COVID-19 diagnosis using AI
- Biosensors and Analytical Detection
- Mass Spectrometry Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Environmental Toxicology and Ecotoxicology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Advanced Chemical Sensor Technologies
- Marine and coastal ecosystems
- Water Treatment and Disinfection
- Analytical Chemistry and Chromatography
- Pharmacological Effects and Assays
- COVID-19 Clinical Research Studies
- Healthcare Facilities Design and Sustainability
- Respiratory viral infections research
- Water Quality and Pollution Assessment
- Environmental Chemistry and Analysis
- Microplastics and Plastic Pollution
- Nicotinic Acetylcholine Receptors Study
- Pesticide Residue Analysis and Safety
- Hormonal and reproductive studies
- Recycling and Waste Management Techniques
- Pharmaceutical and Antibiotic Environmental Impacts
Istituto Superiore di Sanità
2013-2024
National Institute of Health
2017
New SARS-CoV-2 mutations are constantly emerging, raising concerns of increased transmissibility, virulence or escape from host immune response. We describe a nested RT-PCR assay (~1500 bps) to detect multiple nucleotide changes resulting in key spike protein distinctive the major known circulating variants, including three Variants Concern (VOCs) 20I/501Y.V1 (United Kingdom), 20H/501Y.V2 (South Africa), and 20 J/501Y.V3 (Brazil), as well 20E.EU1 variant (Spain), CAL.20C recently identified...
The outbreak of coronavirus infectious disease-2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), has rapidly spread throughout world. Several studies have shown that detecting SARS-CoV-2 in untreated wastewater can be a useful tool to identify new outbreaks, establish trends, and assess prevalence infections. On 06 May 2021, over year into pandemic, we conducted scoping review aiming summarize research data on sewage. Papers dealing with raw sewage...
Environmental surveillance can be a complementary tool for detecting pathogens circulating in communities. We detected monkeypox virus DNA wastewater from Italy's largest airport by using real-time PCR assays targeting the G2R region and F3L N3R genes sequencing. Wastewater quickly adapted to investigate emerging threats.
This study adds insight regarding the occurrence and spread of SARS-CoV-2 Variants Concern (VOCs) Interest (VOIs) in Italy October November 2022, by testing urban wastewater collected throughout country. A total 332 samples were from 20 Italian Regions/Autonomous Provinces (APs) within framework national environmental surveillance. Of these, 164 first week 168 November. ∼1600 bp fragment spike protein was sequenced Sanger (for individual samples) long-read nanopore sequencing pooled...
In recent years, microplastic pollution has been a hot topic as these compounds have used in various production contexts such health, food or technology due to their chemical and physical properties “shelf-life,” making them almost indispensable products daily life. On the other hand, they negative impact on environment and, consequently, biota human health. It is therefore necessary assess actual presence of microplastics drinking water by analysing real samples order estimate possible...
The spread of antibiotic resistant bacteria (ARB) and resistance genes (ARGs) in humans, animals environment is a growing threat to public health. Wastewater treatment plants (WWTPs) are crucial mitigating the risk environmental contamination by effectively removing contaminants before discharge. However, persistence ARB ARGs even after challenge for management water system. To comprehensively assess antimicrobial dynamics, we conducted one-year monitoring study three WWTPs central Italy,...
There is increasing evidence of the use wastewater-based epidemiology to integrate conventional monitoring assessing disease symptoms and signs viruses in a specific territory. We present results SARS-CoV-2 environmental surveillance activity wastewater samples collected between September 2020 July 2021 9 treatment plants (WTPs) located central western Sicily, serving over 570,000 residents. The presence SARS-CoV-2, determined 206 using RT-qPCR assays, was correlated with notified...
ABSTRACT Several studies have demonstrated the advantages of environmental surveillance through monitoring sewer systems for assessment viruses circulating in a given community (wastewater-based epidemiology, WBE). During COVID-19 public health emergency, many reports described presence SARS-CoV-2 RNA stools from patients, and few reported occurrence wastewaters worldwide. Italy is among world’s worst-affected countries pandemic, but so far there are no assessing Italian wastewaters. To this...
The emergence of SARS-CoV-2 variants concern (VOCs) and interest (VOIs) poses an increased risk to global public health underlines the need prioritise monitoring research better respond COVID-19 pandemic. Wastewater can be used monitor spread track variants. A long read amplicon sequencing approach based on Oxford Nanopore technology, targeting spike protein, was applied detect in sewage samples collected central Italy April 2021. Next-generation performed three pooled samples. For variant...
According to Italian Ministerial Decree No. 185 of 12 June 2003, water is considered suitable for irrigation if levels fecal bacteria (i.e., Escherichia coli and Salmonella) are within certain parameters. The detection other microorganisms not required. aim this study determine the bacteriological quality groundwater used occurrence enteric viruses (Norovirus, Enterovirus, Rotavirus, Hepatovirus A), compare presence with indicators. A total 182 wells was analyzed. Widespread contamination...
ABSTRACT Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is responsible for the coronavirus disease COVID-19, a public health emergency worldwide, and Italy among world’s first most severely affected countries. The autochthonous Italian case of COVID-19 was documented on February 21. We investigated possibility that SARS-CoV-2 emerged in earlier than date, by analysing 40 composite influent wastewater samples collected - framework other wastewater-based epidemiology projects...
Wastewater-based epidemiology is a well-established tool for detecting and monitoring the spread of enteric pathogens use illegal drugs in communities real time. Since only few studies Italy have investigated correlation between SARS-CoV-2 wastewater prevalence COVID-19 cases from clinical testing, we conducted one-year surveillance study Sicily to correlate load RNA reported cumulative 14 cities October 2021 September 2022. Furthermore, role variants subvariants increase number infections....
An extraordinary bloom of Planktothrix rubescens, which can produce microcystins (MCs), was observed in early 2009 the Occhito basin, used even as a source drinking water Southern Italy. Several activities, coordinated by task force, were implemented to assess and manage risk associated contaminated cyanobacteria. Main actions were: evaluation analytical protocols for screening confirmatory purpose, monitoring supply chain, training operators, dedicated web site communication. ELISA assay...
The COVID-19 pandemic started in China early December 2019, and quickly spread around the world. epidemic gradually Italy at end of February 2020, by May 31, 232,664 cases 33,340 deaths were confirmed. As a result this pandemic, Italian Ministerial Decree issued on March 11, enforced lockdown; therefore, many social, recreational, cultural centers remained closed for months. In Apulia (southern Italy), all non-urgent hospital activities suspended, some wards closed, with consequent reduction...