A5059516403- Bacillus and Francisella bacterial research
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- Zoonotic diseases and public health
- Bacterial Identification and Susceptibility Testing
- Identification and Quantification in Food
- Yersinia bacterium, plague, ectoparasites research
- Sports Performance and Training
- Liver Disease and Transplantation
- Insect and Pesticide Research
- Dermatological diseases and infestations
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Study of Mite Species
- Vector-borne infectious diseases
- Viral gastroenteritis research and epidemiology
- Martial Arts: Techniques, Psychology, and Education
- Salmonella and Campylobacter epidemiology
- SARS-CoV-2 detection and testing
- Lower Extremity Biomechanics and Pathologies
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata
2016-2025
ANT Foundation Italy Onlus
2023
University of Teramo
2019
University of Milan
2008-2014
University of Bari Aldo Moro
2009
University of Naples Federico II
1985-2003
University of Campania "Luigi Vanvitelli"
2003
New SARS-CoV-2 mutations are constantly emerging, raising concerns of increased transmissibility, virulence or escape from host immune response. We describe a nested RT-PCR assay (~1500 bps) to detect multiple nucleotide changes resulting in key spike protein distinctive the major known circulating variants, including three Variants Concern (VOCs) 20I/501Y.V1 (United Kingdom), 20H/501Y.V2 (South Africa), and 20 J/501Y.V3 (Brazil), as well 20E.EU1 variant (Spain), CAL.20C recently identified...
The poultry red mite (PRM), Dermanyssus gallinae, is a nonburrowing haematophagous nest-dwelling ectoparasite of birds; occasionally it bites humans, inducing dermatitis. possibility that this parasite may also be involved in transmission pathogens an additional concern. We investigated the presence zoonotic agents PRMs from bird nests and pets, related them to urban outbreaks A total 98 12 PRM dermatitis occurred Italian cities 2001 2017 were molecularly for detection Coxiella spp. (16S...
Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry (MALDI-TOF MS) technology is currently increasingly used in diagnostic laboratories as a cost effective, rapid and reliable routine technique for the identification typing of microorganisms. In this study, we MALDI-TOF MS to analyze collection 160 strains belonging Bacillus cereus group (57 B. anthracis, 49 cereus, 1 mycoides, 18 wiedmannii, 27 thuringiensis, 7 toyonensis weihenstephanensis) detect specific...
Background. The highly variable manifestation of the COVID-19 disease, from completely asymptomatic to fatal, is both a clinical and public health issue. criteria for discharge hospitalized patients have been based so far on negative result Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) tests, but persistence viral fragments may exceed that integral virus by weeks. aim our study was verify clearance at culture in clinically recovered are still positive nasopharyngeal...
Bacillus cereus is a gram-positive bacterium widespread in the environment, especially soil and dust. It produces two types of toxins causing vomiting diarrhea. Nowadays, food borne outbreaks due to group bacteria (especially sensu stricto) are increased, as reported by European Food Safety Authority, representing very huge problem agri-food chain. In this work, we analyzed 118 strains belonging group, isolated from several sources (fruit vegetables, dairy products, bakery products)...
Anthrax is a zoonotic disease characterized by rapid onset with usual fatal outcomes in livestock and wildlife. In Ethiopia, anthrax persistent disease; however, there are limited data on the isolation molecular characterization of Bacillus anthracis strains. This study aimed to characterize B. isolated from animal outbreaks between 2019 2024, different localities Ethiopia. was identified using standard microbiology techniques confirmed real-time PCR. For first time genetic diversity five...
The emergence of colistin-resistant Enterobacteriaceae in food products is a growing concern due to the potential transfer resistance human pathogens. This study aimed assess prevalence raw and ready-to-eat samples collected from two regions Italy (Apulia Basilicata) evaluate their phenotypes genetic characteristics. A total 1000 were screened, with 4.4% Enterobacteriaceae. majority isolates belonged Enterobacter spp. (60%), followed by Moellerella wisconsensis, Atlantibacter hermannii,...
Salmonellosis is one of the most frequent foodborne zoonoses, and consumption contaminated poultry meat considered main source Salmonella infections in humans. From 2021 to 2023, 384 chicken samples were collected from retailers Apulia Basilicata regions analysed at Istituto Zooprofilattico Sperimentale della Puglia e (IZSPB) laboratories. The isolates investigated evaluate their phenotypic characteristics antimicrobial resistance. A total 125 (32.55%) tested positive for spp. Three...
Actinobacillus seminis is a gram-negative bacterium that affects reproductive organs, causing epididymitis, low fertility, and occasional abortions in sheep goats. The virulence factors the pathogenicity mechanisms of A. have not been clearly elucidated yet. aim this study to report laboratory investigation performed on farm Basilicata region (southern Italy) following cases abortion. Four samples aborted fetuses taken from two were analyzed investigate main pathogens by bacterial isolation....
Background/Objectives: Antimicrobial resistance (AMR) is a major global health concern with profound implications for human, animal, and environmental health. Marine ecosystems are emerging as reservoirs of resistant bacteria due to contamination from anthropogenic activities. This study aimed investigate fluoroquinolone-resistant multidrug-resistant in loggerhead sea turtles (Caretta caretta). Methods: Cloacal swabs were collected 28 at rescue center southern Italy. Swabs cultured nutrient...
Abstract Background The European region achieved interruption of malaria transmission during the 1970s. Since then, control programs were replaced by surveillance systems in order to prevent possible re-emergence this disease. Sporadic cases non-imported recorded several countries past decade and locally transmitted outbreaks Plasmodium vivax , most probably supported Anopheles sacharovi have been repeatedly reported from Greece since 2009. possibility locally-transmitted has extensively...
Bacillus cereus is isolated from a variety of foods where it may cause food spoilage and/or poisoning due to its toxigenic and pathogenic nature. In this study, we identified members B. groups in 65% the ice cream samples analyzed, which were characterized based on multi locus variable number tandem repeats analysis (MLVA) whole genome sequencing (WGS). The MLVA revealed that 36 strains showed different allelic profiles. Analyses WGS data enabled identification three group: sensu stricto,...
Fourier transform infrared spectroscopy (FTIRS) is a diagnostic technique historically used in the microbiological field for characterization of bacterial strains relation to specific composition their lipid, protein, and polysaccharide components. For each strain, it possible obtain unique absorption spectrum that represents fingerprint obtained based on components outer cell membrane. In this study, FTIRS was applied first time as an experimental tool discrimination two pathogenic species...
Whole-genome sequencing (WGS) has been established for bacterial subtyping and is regularly used to study pathogen transmission, investigate outbreaks, perform routine surveillance. Core-genome multilocus sequence typing (cgMLST) a method that uses WGS data provide high-resolution strain characterization. This aimed at developing novel cgMLST scheme Bacillus anthracis, notorious causes anthrax in livestock humans worldwide. The comprises 3,803 genes were conserved 57 B. anthracis genomes...
The coronavirus disease 2019 (Covid-19) pandemic is caused by the severe acute respiratory syndrome 2 (SARS-CoV-2) and presents a global health emergency that needs urgent intervention. Viruses constantly change through mutation, new variants of virus are expected to occur over time. In United Kingdom (UK), variant called B.1.1.7 has emerged with an unusually large number mutations. aim this study evaluate level protection sera from 12 patients infected later healed in Apulia Region (Italy)...
In Italy anthrax is an endemic disease, with a few outbreaks occurring almost every year. We surveyed 234 B. anthracis strains from animals (n = 196), humans 3) and the environment 35) isolated during Italian in years 1972-2018. Despite considerable genetic homogeneity of anthracis, were effectively differentiated using canonical single nucleotide polymorphisms (CanSNPs) assay multiple-locus variable-number tandem repeat analysis (MLVA). The phylogenetic identity was determined through...
Yersiniosis is the third most reported food-borne zoonosis in Europe. The aim of present study was to perform search for Yersinia enterocolitica food samples collected from Apulia and Basilicata regions (Southern Italy) characterize any isolates by classical modern analytical methods. A total 130 were analyzed between July 2018 2019: them raw milk dairy products made it. Furthermore, 8 out individual bovines reared a Brucella-free farm which showed false positive serological reaction...