A5091674759- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Plant Virus Research Studies
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Enzyme Production and Characterization
- Antimicrobial Resistance in Staphylococcus
- Cytomegalovirus and herpesvirus research
- Virology and Viral Diseases
- Pneumonia and Respiratory Infections
- Hearing, Cochlea, Tinnitus, Genetics
- Immune Response and Inflammation
- Respiratory viral infections research
- Viral Infections and Immunology Research
- Molecular spectroscopy and chirality
- Antibiotic Resistance in Bacteria
- Microbial Natural Products and Biosynthesis
- Hearing Loss and Rehabilitation
- Cancer Research and Treatments
- Metal Extraction and Bioleaching
- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Microbial Community Ecology and Physiology
University of Naples Federico II
2017-2024
Federico II University Hospital
2022-2023
Aix-Marseille Université
2017-2022
Institut de Microbiologie de la Méditerranée
2021-2022
The species- and clone-specific susceptibility of Staphylococcus cells for bacteriophages is governed by the structures glycosylation patterns wall teichoic acid (WTA) glycopolymers. glycosylation-dependent phage-WTA interactions in opportunistic pathogen epidermidis other coagulase-negative staphylococci (CoNS) have remained unknown. We report a new S. WTA glycosyltransferase TagE whose deletion confers resistance to siphoviruses such as ΦE72 but enables binding otherwise unbound...
The mimivirus 1.2 Mb genome was shown to be organized into a nucleocapsid-like genomic fiber encased in the nucleoid compartment inside icosahedral capsid. protein shell is composed of mixture two GMC-oxidoreductase paralogs, one them being main component glycosylated layer fibrils at surface virion. In this study, we determined effect deletion each corresponding genes on and fibrils. First, deleted GMC-oxidoreductase, most abundant fiber, its structure composition mutant. As expected, it...
Nonulosonic acids are found in the surface polysaccharides of many bacterial species and often implicated pathogenesis. Here, structure a novel 5,7-diacetamido-3,5,7,9-tetradeoxynon-2-ulosonic acid recovered from capsular polysaccharide multiply antibiotic resistant Acinetobacter baumannii isolate was determined. The carries sugar synthesis module that differs by only single gene for 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-altro-non-2-ulosonic or 5,7-di-N-acetylacinetaminic acid,...
The general perception of viruses is that they are small in terms size and genome, hijack the host machinery to glycosylate their capsid. Giant subvert all these concepts: particles not small, genome more complex than some bacteria. Regarding glycosylation, this concept has been already challenged by finding Chloroviruses have an autonomous glycosylation produces oligosaccharides similar those (6-12 units), albeit different structure compared viral counterparts. We report herein Mimivirus...
The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for study of their glycosylation machinery. We focused on proposed Megavirinae subfamily, which glycan-related genes were code involved in layer fibrils surrounding icosahedral capsids. compared compositions corresponding biosynthetic pathways among clade members using a combination chemical bioinformatics approaches. first demonstrated differs many aspects from what was previously...
The giant virus Mimivirus encodes an autonomous glycosylation system that is thought to be responsible for the formation of complex and unusual glycans composing fibers surrounding its icosahedral capsid, including dideoxyhexose viosamine. Previous studies have identified a gene cluster in genome, encoding enzymes involved nucleotide-sugar production glycan formation, but functional characterization these full identification found viral remain incomplete. Because viosamine typically acylated...
Fusobacterium nucleatum is a common member of the oral microbiota. However, this symbiont has been found to play an active role in disease development. As Gram-negative bacterium, F. protective outer membrane layer whose external leaflet mainly composed lipopolysaccharides (LPSs). LPSs crucial interaction between bacteria and host immune system. Here, we characterised structure O-antigen lipid A from ssp. animalis ATCC 51191 by using combination GC-MS, MALDI NMR techniques. The results...
Abstract The species- and clone-specific susceptibility of Staphylococcus cells for bacteriophages is governed by the structures glycosylation patterns wall teichoic acid (WTA) glycopolymers. glycocodes phage-WTA interaction in opportunistic pathogen epidermidis other coagulase-negative staphylococci (CoNS) have remained unknown. We report a new S. WTA glycosyltransferase TagE whose deletion confers resistance to siphoviruses such as ΦE72 but enables binding otherwise unbound podoviruses....
Moraxella ovis is a Gram-negative bacterium isolated from sheep conjunctivitis cases and rare isolate of infectious bovine keratoconjunctivitis (IBK). This species closely related to M. bovoculi, another which can also be IBK, or cattle upper respiratory tract (URT). Prior molecular identification techniques, bovoculi was frequently misclassified as ovis. We previously described the structure two oligosaccharides (lipooligosaccharide-derived, minor major glycoforms) 237T (type strain, ATCC...
Understanding the molecular mechanisms which drive and modulate host-pathogen interactions are essential when designing effective therapeutic diagnostic approaches aimed at controlling infectious diseases. Certain large giant viruses have recently been discovered as components of human virome, yet little is known about their with host immune system. We dissected role viral N-linked glycans during interaction between glycoproteins from six chloroviruses (belonging to three chlorovirus...
The structures of the four N-linked glycans from prototype chlorovirus PBCV-1 major capsid protein do not resemble any other in three domains life. All known chloroviruses and antigenic variants (or mutants) share a unique conserved central glycan core consisting five sugars, except for mutant virus P1L6, which has sugars. A combination genetic structural analyses indicates that coded by gene a111/114r, all chloroviruses, is glycosyltransferase with putative approximately 300 amino acids...
Asparagine-linked protein glycosylations (N-glycosylations) are one of the most abundant post-translational modifications and essential for various biological phenomena. Herein, we describe isolation, structural determination, chemical synthesis N-glycan from hyperthermophilic archaeon Thermococcus kodakarensis. The organism possesses a unique structure including myo-inositol, which has not been found in previously characterized N-glycans. In this structure, myo-inositol is highly...
Paramecium bursaria chlorella virus MA-1D is a chlorovirus that infects Chlorella variabilis strain NC64A, symbiont of the protozoan bursaria. has 339-kb genome encoding ca. 366 proteins and 11 tRNAs. Like other chloroviruses, its major capsid protein (MCP) decorated with N-glycans, whose structures have been solved in this work by using nuclear magnetic spectroscopy matrix-assisted laser desorption ionization-time flight mass spectrometry along MS/MS experiments. This analysis identified...
Protein A075L is a β-xylosyltransferase that participates in producing the core of N-glycans found VP54, major viral capsid protein Paramecium bursaria chlorella virus-1 (PBCV-1). In this study, we present an X-ray crystallographic analysis apo form A075L, along with its complexes sugar donor and trisaccharide acceptor. The structure shows typical GT-B folding, two Rossmann-like fold domains, which acceptor substrate binds to N-terminal region, nucleotide-sugar C-terminal region. We propose...
Abstract The mimivirus 1.2Mb genome was shown to be organized into a nucleocapsid-like genomic fiber encased in the nucleoid compartment inside icosahedral capsid (1). protein shell is composed of mixture two GMC-oxidoreductase paralogs, one them being main component glycosylated layer fibrils at surface virion (2). In this study, we determined effect deletion each corresponding genes on and fibrils. First, deleted most abundant fiber, its structure composition mutant. As expected, it second...
Abstract The general perception of viruses is that they are small in terms size and genome, hijack the host machinery to glycosylate their capsid. Giant subvert all these concepts: particles not small, genome more complex than some bacteria. Regarding glycosylation, this concept has been already challenged by finding Chloroviruses have an autonomous glycosylation produces oligosaccharides similar those (6–12 units), albeit different structure compared viral counterparts. We report herein...
Abstract Asparagine‐linked protein glycosylations ( N ‐glycosylations) are one of the most abundant post‐translational modifications and essential for various biological phenomena. Herein, we describe isolation, structural determination, chemical synthesis ‐glycan from hyperthermophilic archaeon Thermococcus kodakarensis . The organism possesses a unique structure including myo ‐inositol, which has not been found in previously characterized ‐glycans. In this structure, ‐inositol is highly...