A5037375687- Carbohydrate Chemistry and Synthesis
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Infections and bacterial resistance
- Enzyme Production and Characterization
- Plant Pathogenic Bacteria Studies
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- Polyamine Metabolism and Applications
- Vibrio bacteria research studies
- Methane Hydrates and Related Phenomena
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Escherichia coli research studies
- Plant-Microbe Interactions and Immunity
- Mycobacterium research and diagnosis
- Amino Acid Enzymes and Metabolism
- Glycosylation and Glycoproteins Research
- Radioactive element chemistry and processing
- Pneumocystis jirovecii pneumonia detection and treatment
- Antibiotics Pharmacokinetics and Efficacy
N.D. Zelinsky Institute of Organic Chemistry
2017-2024
D. Mendeleyev University of Chemical Technology of Russia
2017-2019
Russian Academy of Sciences
2017-2019
Acinetobacter baumannii is one of the most clinically important nosocomial pathogens. The World Health Organisation refers it to its «critical priority» category develop new strategies for effective therapy. This microorganism capable producing structurally diverse capsular polysaccharides (CPSs), which serve as primary receptors A. bacteriophages carrying polysaccharide-depolymerasing enzymes. In this study, eight novel bacterial viruses that specifically infect strains belonging K2/K93,...
The type of capsular polysaccharide (CPS) on the cell surface Acinetobacter baumannii can determine specificity lytic bacteriophage under consideration for therapeutic use. Here, we report isolation a phage an extensively antibiotic resistant ST2 A. isolate AB5001 that carries KL3 CPS biosynthesis gene cluster predicting K3-type CPS. As did not infect isolates carrying or KL22 and known to produce K3 CPS, structure isolated from was determined. produced variant form, K3-v1, lacks β-d-GlсpNAc...
Acinetobacter baumannii isolate NIPH 329 carries a novel capsular polysaccharide (CPS) gene cluster, designated KL46, that is closely related to the KL5 locus in A. SDF but includes genes for synthesis of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic (di-N-acetylpseudaminic) acid (Pse5Ac7Ac) instead corresponding D-glycero-D-galacto isomer (di-N-acetyllegionaminic acid) (Leg5Ac7Ac). In agreement with genetic content chemical studies K46 CPS produced by revealed branched...
The structure of the K141 type capsular polysaccharide (CPS) produced by Acinetobacter baumannii KZ1106, a clinical isolate recovered from Kazakhstan in 2016, was established sugar analyses and one- two-dimensional
The whole-genome sequence of the multiply antibiotic resistant Acinetobacter baumannii isolate RCH51 belonging to type ST103 (Institut Pasteur scheme) revealed that set genes at capsule locus, KL24, includes four predicted direct synthesis 3-acetamido-3,6-dideoxy-d-galactose (d-Fuc3NAc), and this sugar was found in capsular polysaccharide (CPS). One these genes, fdtE, encodes a novel bifunctional protein with an N-terminal FdtA 3,4-ketoisomerase domain C-terminal acetyltransferase domain....
Infections caused by Acinetobacter baumannii isolates from the major global clones, GC1 and GC2, are difficult to treat with antibiotics, phage therapy, which requires extensive knowledge of variation in surface polysaccharides, is an option under consideration. The gene clusters directing synthesis capsular polysaccharide (CPS) A. isolate A388 GC2 G21 differ a single glycosyltransferase (gtr) gene. They include genes encoding novel UDP-glucose dehydrogenase (Ugd2) putative pyruvyl...
A comprehensive understanding of capsular polysaccharide (CPS) diversity is critical to implementation phage therapy treat panresistant Acinetobacter baumannii infections. Predictions from genome sequences can assist identification the CPS type but be complicated if genes outside K locus (CPS biosynthesis gene cluster) are involved. Here, produced by A. clinical isolate 36-1454 carrying a novel locus, KL127, was determined and compared other CPSs. KL127 differs KL128 in only two...
Bacteriophage show promise for the treatment of Acinetobacter baumannii infections that resist all therapeutically suitable antibiotics. Many tail-spike depolymerases encoded by phage are able to degrade A. capsular polysaccharide (CPS) exhibit specificity linkage present between K-units make up CPS polymers. This is formed a specific Wzy polymerase, and ability predict this using sequence-based methods identify at K locus could assist with selection therapy. However, little known about...
The K239 type capsular polysaccharide (CPS) isolated from Acinetobacter baumannii isolate MAR19-4435 was studied by sugar analysis, one- and two-dimensional 1H 13C NMR spectroscopy. consists of branched heptasaccharide repeats (K-units) comprised five residues l-rhamnose (l-Rhap), one residue each d-glucuronic acid (d-GlcpA) N-acetyl-d-glucosamine (d-GlcpNAc). structure is closely related to that the A. K86 CPS type, though two differ in 2,3-substitution patterns on l-Rhap involved linkage...
ABSTRACT The carbapenem-resistant Acinetobacter baumannii isolate BAL062 is a clinical reference used in several recent experimental studies. It from ventilator-associated pneumonia (VAP) patient an intensive care unit at the Hospital for Tropical Diseases (HTD), Ho Chi Minh City, Vietnam 2009. Here, was found to belong B sub-lineage of global clone 2 (GC2) isolates previously reported outbreak (2008 and 2012) VAP A. HTD. While related were extensively antibiotic-resistant carry...
Klebsiella pneumoniae is a pathogen associated with various infection types, which often exhibits multiple antibiotic resistance. Phages, or bacterial viruses, have an ability to specifically target and destroy K. pneumoniae, offering potential means of combatting multidrug-resistant infections. Phage enzymes are another promising therapeutic agent that can break down capsular polysaccharide, shields from the immune response external factors. In this study, phage K5 was isolated; active...
The structures of capsular polysaccharides (CPSs) produced by different Acinetobacter baumannii strains have proven to be invaluable in confirming the role specific genes synthesis rare sugars through correlation genetic content at CPS biosynthesis locus with found corresponding structures. A module four (rmlA, rmlB, vioA and vioB) was identified KL57 capsule gene cluster A. isolate BAL_212 from Vietnam. These were predicted direct 4-acetamido-4,6-dideoxy-d-glucose (N-acetylviosamine,...
The clonal bacterial species Acinetobacter baumannii is an emerging multidrug-resistant pathogen which causes high-lethality infections. Cells of A. are surrounded by the type-specific capsular polysaccharide (CPS), provides resistance to protective mechanisms host and considered a target for immunization. conjugates three inert carrier proteins type K9 CPS fragments, contained various numbers oligosaccharide repeats (K-units), were synthesized periodate oxidation squaric acid chemistry....
Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at chromosomal K locus. The clusters are related to previously described KL11 KL83 sharing genes for synthesis l-rhamnose (l-Rhap) 6-deoxy-l-talose (l-6dTalp). CPS material isolated from MAR24 was studied by sugar analysis Smith degradation along with one- two-dimensional 1H 13C NMR...