A5028891344- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Helminth infection and control
- Immune Cell Function and Interaction
- Pancreatic and Hepatic Oncology Research
- Coccidia and coccidiosis research
- Multiple Sclerosis Research Studies
- Crystallography and molecular interactions
- Parasites and Host Interactions
- Cancer Immunotherapy and Biomarkers
- Ubiquitin and proteasome pathways
- Carbohydrate Chemistry and Synthesis
- Single-cell and spatial transcriptomics
- Tryptophan and brain disorders
- Enzyme Production and Characterization
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
Amsterdam University Medical Centers
2018-2025
Vrije Universiteit Amsterdam
2018-2025
Oncode Institute
2022-2025
Cancer Center Amsterdam
2020-2025
Texas Christian University
2024
Amsterdam UMC Location Vrije Universiteit Amsterdam
2018-2023
Amsterdam Neuroscience
2021-2022
Institute of Infection and Immunity
2022
Universidad de la República
2017-2018
Centro Uruguayo de Imagenología Molecular
2015-2018
Changes in glycosylation during tumour progression are a key hallmark of cancer. One the glycan moieties generally overexpressed cancer sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors. We here show that Pancreatic Ductal Adenocarcinoma (PDAC) cells present an increased sialylation be recognized by Siglec-7 and Siglec-9 on myeloid cells. identified expression α2,3 sialyltransferases ST3GAL1 ST3GAL4 as main contributor synthesis ligands for Analysing...
Abstract The immediate call for translational research in the field of coronavirus disease (COVID-19) pandemic, needs new and unexplored angles to support contribute this important worldwide health problem. aim study is better understand pathogenic mechanisms underlying COVID-19, deciphering carbohydrate-mediated interactions SARS-CoV-2 spike protein. We studied carbohydrate-binding receptors that could be viral entry immune-modulatory responses, we protein with host lung microbiota....
Glioblastoma is the most aggressive brain malignancy, for which immunotherapy has failed to prolong survival. Glioblastoma-associated immune infiltrates are dominated by tumor-associated macrophages and microglia (TAMs), key mediators of suppression resistance immunotherapy. We others demonstrated aberrant expression glycans in different cancer types. These trigger inhibitory signaling TAMs through glycan-binding receptors. investigated glioblastoma glycocalyx as a tumor-intrinsic...
Abstract Despite recent advances in cancer immunotherapy, pancreatic ductal adenocarcinoma (PDAC) remains unresponsive due to an immunosuppressive tumor microenvironment, which is characterized by the abundance of cancer-associated fibroblasts (CAFs). Once identified, CAF-mediated immune inhibitory mechanisms could be exploited for immunotherapy. Siglec receptors are increasingly recognized as checkpoints, and their ligands, sialic acids, known overexpressed cells. Here, we unveil a...
Changes in glycosylation patterns have been associated with malignant transformation and clinical outcomes several cancer types, prompting ongoing research into the mechanisms involved potential applications. In this study, we performed an extensive transcriptomic analysis of glycosylation-related genes pathways, using publicly available bulk single cell datasets from tumor samples lines. We identified pathways strongly different which may represent novel diagnostic biomarkers. By RNA-seq...
Significance Current immunotherapies only benefit a minority of all cancer patients, so it is necessary to develop other strategies boost patients’ antitumor CD8 + T cell responses. Here, we formulated liposomal vaccine carrier that selectively delivers tumor antigens and Toll-like receptor agonists human CD169/Siglec-1 antigen-presenting cells (APCs) through the incorporation gangliosides are natural ligands CD169. This binds variety CD169 APCs, including monocyte-derived dendritic (moDCs)...
Background PD1 immune checkpoint blockade (αPD1 ICB) has shown unparalleled success in treating many types of cancer. However, response to treatment does not always lead tumor rejection. While αPD1 ICB relies on cytotoxic CD8 + T cells, antigen-presenting cells (APCs) at the site are also needed for costimulation tumor-infiltrating lymphocytes (TILs). It is still unclear how these APCs develop and function before during or they associated with Methods Here, we used B16 mouse melanoma MC38...
Monocytes are antigen-presenting cells (APCs) that play diverse roles in promoting or regulating inflammatory responses, but their role T cell stimulation is not well defined. In conditions, monocytes frequently show increased expression of CD169/Siglec-1, a type-I interferon (IFN-I)-regulated protein. However, little known about the phenotype and function these CD169 + monocytes. Here, we have investigated human different diseases, capacity to activate CD8 cells, potential for...
Multiple sclerosis (MS) is a chronic demyelinating disease characterised by immune cell infiltration resulting in lesions that preferentially affect periventricular areas of the brain. Despite research efforts to define role various cells MS pathogenesis, focus has been on few populations while full-spectrum analysis, encompassing others such as natural killer (NK) cells, not performed. Here, we used single-cell mass cytometry (CyTOF) profile landscape brain – septum and choroid plexus...
Background Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers. Despite successful application immune checkpoint blockade in a range human cancers, immunotherapy PDAC remains unsuccessful. characterized by desmoplastic, hypoxic and highly immunosuppressive tumor microenvironment (TME), where T-cell infiltration often lacking (immune desert), or T cells are located distant from islands excluded). Converting TME to an immune-inflamed state, allowing infiltration, could...
Abstract Background Recent studies suggest that extended interval dosing of ocrelizumab, an anti-B cell therapy, does not affect its clinical effectiveness in most patients with multiple sclerosis (MS). However, it remains to be established whether certain B subsets are differentially repopulated after different intervals and these relate efficacy. Methods We performed high-dimensional single-cell characterization the peripheral immune landscape MS standard (SID; n = 43) or (EID; 37)...
Abstract Background Alzheimer’s disease (AD) is the most frequent cause of dementia. Recent evidence suggests involvement peripheral immune cells in disease, but underlying mechanisms remain unclear. Methods We comprehensively mapped changes AD patients with mild cognitive impairment (MCI) or dementia compared to controls, using cytometry by time-of-flight (CyTOF). Results found an adaptive signature AD, and specifically highlight accumulation PD1 + CD57 CD8 T effector memory re-expressing...
Helminths express various carbohydrate-containing glycoconjugates on their surface, and they release glycan-rich excretion/secretion products that can be very important in life cycles, infection pathology. Recent evidence suggests parasite could play a role the evasion of immune response, leading to modified Th2-polarized response favors survival host. Nevertheless, there is limited information about nature or function glycans produced by trematode Fasciola hepatica, causative agent...
Resistance to late blight in potato is either qualitative or quantitative nature. The resistance durable, but the molecular and biochemical mechanisms underlying are poorly understood, not efficiently utilised breeding. A non-targeted metabolomics, using high resolution hybrid mass spectrometry, was applied decipher of advanced breeding diploid genotypes (Solanum tuberosum L. Group Phureja), with valuable sources genetic diversity. metabolomics profiles resistant (AC04 AC09) were compared a...
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive malignancies with a 5-year survival rate only 9%. Despite fact that changes in glycosylation patterns during tumour progression have been reported, no systematic approach has conducted to evaluate its potential for patient stratification. By analysing publicly available transcriptomic data samples and cell lines, we identified here two specific glycan profiles PDAC correlated progression, clinical outcome epithelial...
There is substantial evidence suggesting that certain parasites can have antitumor properties. We evaluated mucin peptides derived from the helminth Echinococcus granulosus (denominated Egmuc) as potential inducers of activity. present data showing Egmuc were capable inducing an increase activated NK cells in spleen immunized mice, a fact was correlated with capacity splenocytes to mediate killing tumor cells. demonstrated enhance LPS-induced maturation dendritic vitro by increasing...
Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) expressed on a variety of DCs, is C-type lectin receptor that recognizes glycans diverse range pathogens, including parasites. The interaction DC-SIGN with pathogens triggers specific signaling events modulate DC-maturation and activity regulate T-cell activation by DCs. In this work we evaluate whether F. hepatica can immune DCs via DC-SIGN. We demonstrate interacts glycoconjugates through mannose fucose residues. also show...
Multiple sclerosis (MS) involves a misdirected immune attack against myelin in the brain and spinal cord, leading to profound neuroinflammation neurodegeneration. While mechanisms of disease pathogenesis have been widely studied, suppression that lead resolution autoimmune response are still poorly understood. Here, we investigated role C-type lectin receptor macrophage galactose-type (MGL), usually expressed on tolerogenic antigen-presenting cells (APCs), as negative regulator...
Fasciolosis, caused by Fasciola hepatica and F. gigantica, is a trematode zoonosis of interest in public health livestock production. Like other helminths, modulates the host immune response inducing potent polarized Th2 regulatory T cell responses, down-regulating production Th1 cytokines. In this work we show that glycans increase responses modulating TLR-induced maturation function dendritic cells (DCs). This process was mediated Macrophage Gal/GalNAc Lectin (MGL) expressed on DCs,...
Fasciola hepatica, also known as the liver fluke, is a trematode that infects livestock and humans causing fasciolosis, zoonotic disease of increasing importance due to its worldwide distribution high economic losses. This parasite immunoregulates host immune system by inducing strong Th2 regulatory T response immunomodulating dendritic cell (DC) maturation alternative activation macrophages. In this paper we show F. hepatica infection in mice induces upregulation heme-oxygenase-1 (HO-1),...