A5100665055- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Extracellular vesicles in disease
- Seed and Plant Biochemistry
- RNA Research and Splicing
- Genetic and phenotypic traits in livestock
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Metal-Organic Frameworks: Synthesis and Applications
- Plant nutrient uptake and metabolism
- Plant Pathogens and Fungal Diseases
- Aquaculture Nutrition and Growth
- Animal Genetics and Reproduction
- Protein Hydrolysis and Bioactive Peptides
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Plant Parasitism and Resistance
- Bacterial biofilms and quorum sensing
- Inflammasome and immune disorders
- Microbial Metabolites in Food Biotechnology
Gansu Academy of Agricultural Sciences
2023-2025
Lanzhou University
2020-2025
Shandong University
2022-2025
Guangzhou Medical University
2024
Xi'an Jiaotong University
2024
Harbin Veterinary Research Institute
2019-2023
Sichuan Agricultural University
2023
Nanchang University
2023
First Affiliated Hospital of Nanchang University
2023
Chinese Academy of Agricultural Sciences
2019-2023
We report here the first genome-wide functional genomic screen for longevity genes. systematically surveyed Caenorhabditis elegans genes using large-scale RNA interference (RNAi), and found that RNAi inactivation of 89 extend C. lifespan. Components daf-2 /insulin-like signaling pathway are recovered, as well regulate metabolism, signal transduction, protein turnover, gene expression. Many these candidate conserved across animal phylogeny. Genetic interaction analyses with new indicate some...
Abstract Arabidopsis thaliana serves as a model species for investigating various aspects of plant biology. However, the contribution genomic structural variations (SVs) and their associate genes to local adaptation this widely distribute remains unclear. Here, we de novo assemble chromosome-level genomes 32 A. ecotypes determine that variable expand gene pool in different thus assist adaptation. We develop graph-based pan-genome identify 61,332 SVs overlap with 18,883 genes, some which are...
Photoluminescent coordination nanosheets (CONASHs) comprising three-way terpyridine (tpy) ligands and zinc(II) ions are created by allowing the two constitutive components to react with each other at a liquid/liquid interface. Taking advantage of bottom-up CONASHs, or flexibility in organic ligand design modes, we demonstrate diversity tpy-zinc(II) CONASH structures photofunctions. A combination 1,3,5-tris[4-(4′-2,2′:6′,2″-terpyridyl)phenyl]benzene (1) Zn(BF4)2 affords cationic featuring...
Yak has been subject to natural selection, human domestication and interspecific introgression during its evolution. However, genetic variants favored by each of these processes have not distinguished previously. We constructed a graph-genome for 47 genomes 7 cross-fertile bovine species. This allowed detection 57,432 high-resolution structural (SVs) within across the species, which were genotyped in 386 individuals. evolutionary origins diverse SVs domestic yaks phylogenetic analyses....
Bioactive naturally occurring macrocyclic peptides often exhibit a strong bias for hydrophobic residues. Recent advances in vitro display technologies have made possible the identification of potent peptide ligands to protein targets interest. However, such approaches so far been restricted using libraries composed containing mixtures and hydrophilic/charged amino acids encoded by standard genetic code. In present study, we demonstrated ribosomal expression exotic under radically...
Merging structural variations (SVs) at the population level presents a significant challenge, yet it is essential for conducting comprehensive genotypic analyses, especially in era of pangenomics. Here, we introduce PanPop, tool that utilizes an advanced sequence-aware SV merging algorithm to efficiently merge SVs various types. We demonstrate PanPop can and optimize majority multiallelic into informative biallelic variants. show its superior precision lower rates missing data compared...
Abstract Structural variants (SVs) represent an important genetic resource for both natural and artificial selection. Here we present a chromosome-scale reference genome domestic yak (Bos grunniens) that has longer contigs scaffolds (N50 44.72 114.39 Mb, respectively) than reported any other ruminant genome. We further obtained long-read resequencing data 6 wild 23 yaks constructed SV map of 372,220 SVs covers the geographic range yaks. The majority contains repetitive sequences several are...
Abstract Shotgun sequencing is a high-throughput method used to detect copy number variants (CNVs). Although there are numerous CNV detection tools based on shotgun sequencing, their quality varies significantly, leading performance discrepancies. Therefore, we conducted comprehensive analysis of next-generation sequencing-based over the past decade. Our findings revealed that majority mainstream employ similar rationale: calculates so-called read depth signal from aligned reads and then...
Rationale: Glioblastoma multiforme (GBM), one of the most malignant tumors central nervous system, has a poor prognosis, mainly because its high recurrence caused by rapid development drug resistance to postoperative chemotherapy. Although macroautophagy/autophagy is believed be fundamental factor in tumor survival during chemotherapy, there still lack autophagy biomarkers for predicting patient prognosis and chemotherapeutic efficacy clinical practice. Methods: We combined transcriptomic...
The ARF gene family is crucial for regulating plant growth, development, and responses to various signaling pathways. In this study, 26 quinoa genes (CqARF01–CqARF26) were identified, with encoded proteins varying in length from 553 1092 amino acids, molecular weights 60.66 111.71 kDa, isoelectric points 5.15 8.21. found either the nucleus (13 CqARFs) or cytoplasm (11 CqARFs). Phylogenetic analysis classified into five groups. All CqARFs contained seven conserved motifs (Motif1, Motif2,...
Abstract Chlorophylls function in photosynthesis, and are critical to plant developmental processes responses environmental stimuli. Chlorophyll b is synthesized from chlorophyll a by oxygenase (CAO). Here, we characterize yellow-green leaf (ygl) mutant identify the causal gene which encodes maize (ZmCAO1). A 51 bp Popin transposon insertion ZmCAO1 strongly disrupts its transcription. Low enzyme activity of leads reduced concentrations b, resulting phenotype ygl mutant. The net...
Quinoa is an important economic food crop. However, quinoa seedlings are susceptible to drought stress, and the molecular mechanism of tolerance remains unclear. In this study, we compared transcriptomic physiological analyses drought-tolerant (L1) (HZ1) genotypes exposed 20% PEG for 3 9 days at seedling stage. Compared with HZ1, stress had less damage photosynthetic system, contents SOD, POD CAT were higher H
To characterize an MDR blaIMP-4-harbouring plasmid from Enterobacter cloacae EC62 of swine origin in China. Plasmid pIMP-4-EC62 E. was transferred by conjugation via filter mating into Escherichia coli J53. DNA extracted J53 transconjugant and sequenced using single-molecule real-time (SMRT) technology. MIC values for both the isolate were determined broth microdilution agar dilution methods. stability assessed through a series passages on antibiotic-free media. is 314351 bp length, encodes...
The global dissemination of carbapenem resistance genes is great concern. Animals are usually considered a reservoir and an important source human infection. Although carbapenemase-producing Enterobacteriaceae strains animal origin have been reported increasingly, bla KPC-2 -positive from food-producing animals still rare. In this study, we first describe the isolation characterization carbapenem-resistant Klebsiella pneumoniae ST11 isolate, strain K15, which pig coproduces FosA3 via two...
To characterize an NDM-1-encoding multiresistance IncHI5 plasmid from Enterobacter cloacae complex of chicken origin.Carbapenemase genes were detected by PCR and Sanger sequencing. The MICs for the E. isolate its transformant determined agar dilution broth microdilution methods. Conjugation electrotransformation performed to assess horizontal transferability carbapenemase plasmid. Plasmid DNA was isolated fully sequenced using Illumina HiSeq PacBio platforms. stability investigated...
To identify and characterize a novel tetracycline resistance gene on multiresistance plasmid from Staphylococcus aureus SA01 of chicken origin.MICs were determined by broth microdilution according to CLSI recommendations. The whole genome sequence S. was via Illumina HiSeq Oxford Nanopore platforms followed hybrid assembly. new tet cloned expressed in aureus. functionality the corresponding protein as an efflux pump tested inhibition assays.A gene, tet(63), identified SA01. tet(63)...
Introduction Exposure to antibiotics (ABX) during pregnancy can have a systematic effect on both fetal and maternal health. Although previous biomonitoring studies indicated the effects children of extensive exposure ABX, pregnant women remain scarce. To explore environmental ABX through accidental ingestion identify potential health risks, present study investigated 122 in East China between 2019 2020. Research design methods The presence six categories (quinolones, sulfonamides,...
Abstract A new coumarinolignan, cleomiscosin E ( 1 ), together with the known compound 2 has been isolated from seeds of Brucea javanica (L.) Merr . Their structures were assigned on basis spectral studies. These two compounds exhibited potent anti‐inflammatory activities by inhibiting nitric oxide (NO) production in lipopolysaccharide (LPS)‐activated RAW264.7 macrophages.