A5054007317- Bacteriophages and microbial interactions
- Helicobacter pylori-related gastroenterology studies
- Genomics and Phylogenetic Studies
- Diet, Metabolism, and Disease
- Bacterial biofilms and quorum sensing
- Salmonella and Campylobacter epidemiology
- Galectins and Cancer Biology
- Hibiscus Plant Research Studies
- Plant Virus Research Studies
- Cytokine Signaling Pathways and Interactions
- Moringa oleifera research and applications
- Seaweed-derived Bioactive Compounds
- Fungal Biology and Applications
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Cancer Research and Treatments
- Microbial Metabolites in Food Biotechnology
- Bone Tissue Engineering Materials
- Advanced biosensing and bioanalysis techniques
- Microbial Community Ecology and Physiology
- Viral gastroenteritis research and epidemiology
- S100 Proteins and Annexins
- Aquaculture disease management and microbiota
- Medicinal Plants and Bioactive Compounds
- Advanced Biosensing Techniques and Applications
University of Naples Federico II
2016-2021
Federico II University Hospital
2020
The development of a simple and low cost electrochemical impedance immunosensor based on screen printed gold electrode for rapid detection Escherichia coli in water is reported. fabricated by immobilizing anti-E. antibodies onto surface covalent way the photochemical immobilization technique, procedure able to bind upright surfaces. Impedance spectra are recorded 0.01 M phosphate buffer solution (PBS) containing 10 mM Fe(CN)63−/Fe(CN)64− as redox probe. Nyquist plots can be modelled with...
Periodic epidemics of black rot disease occur worldwide causing substantial yield losses. Xanthomonas campestris pv. (Xcc) represents one the most common bacteria able to cause above in cruciferous plants such as broccoli, cabbage, cauliflower, and Arabidopsis thaliana. In agriculture, several strategies are being developed contain infection. The use bacteriophages could represent a valid efficient approach overcome this widespread phenomenon. Several studies have highlighted potential...
Moringa oleifera Lam. is a fast-growing and drought-resistant tree of the Moringaceae family. The known with some common names: miracle, ben oil, drumstick, horseradish or simply moringa. plant grows in variety environments including harsh conditions. has high content phytonutrients, being used as natural integrators to cure malnutrition. Phytochemical studies organs showed rich source primary secondary metabolites belonging different classes organic compounds. Pharmacological confirmed use...
Helicobacter pylori (H. pylori) is a Gram-negative bacterium which colonizes the human stomach. The ability of H. to evade host defense system and emergence antibiotic resistant strains result in bacteria persistence chronic inflammation, leads both severe gastric extra-gastric diseases. Consequently, innovative approaches able overcome clinical outcomes are needed. In this work, we develop novel non-toxic therapy based on synergistic action phage lactoferrin adsorbed hydroxyapatite...
Pseudomonas fluorescens is an opportunistic, psychotropic pathogen that can live in different environments, such as plant, soil, or water surfaces, and it associated with food spoilage. Bioactive compounds be used antimicrobials added into packaging systems. Quercetin lactoferrin are the best candidates for development of a complex two molecules absorbed on bio combability structure hydroxyapatite. The minimum inhibiting concentration (MIC) single components dropped down MIC value against...
Purpose: The use of bacteriophages represents a valid alternative to conventional antimicrobial treatments, overcoming the widespread bacterial antibiotic resistance phenomenon. In this work, we evaluated whether biomimetic hydroxyapatite (HA) nanocrystals are able enhance some properties bacteriophages. final goal study was demonstrate that HA can be used for bacteriophage delivery in context infections, and contribute – at same time biological such as stability, preservation, activity, so...
Helicobacter pylori (Hp) is a Gram-negative bacterium colonizing the human stomach. Nuclear Magnetic Resonance (NMR) analysis of intracellular gastric carcinoma cells (MKN-28) incubated with Hp cell filtrate (Hpcf) displays high levels amino acids, including branched chain acids (BCAA) isoleucine, leucine, and valine. Polymerase reaction (PCR) Array Technology shows upregulation mammalian Target Of Rapamycin Complex 1 (mTORC1), inflammation, mitochondrial dysfunction. The review literature...
Mushrooms produce a wide range of bioactive polysaccharides, different from each other in chemical structure and biological effects. In the last years, idea to develop functional foods or drugs containing fungal polysaccharides is attracting great attention. Fruiting bodies Basidiomycetes Ganoderma lucidum are commonly used Oriental medicine treat several disorders. G. – mainly β-glucans heteroglycans have numerous properties such as antitumour immunomodulatory activities. This report shows,...
Bacterial biofilm provides bacteria with resistance and protection against conventional antimicrobial agents the host immune system. Bacteriophages are known to move across make it permeable antimicrobials. Mineral hydroxyapatite (HA) can improve lytic activity of bacteriophages, and, together eicosanoic acid (C20:0), destroy structure. Here, we demonstrate efficacy combined use phage, HA C20:0 Xanthomonascampestris pv campestris (Xcc) biofilm. We used nuclear magnetic resonance (NMR)-based...
Biofilm protects bacteria against the host's immune system and adverse environmental conditions. Several studies highlight efficacy of lytic phages in prevention eradication bacterial biofilms. In this study, activity Xccφ1 (Xanthomonas campestris pv. campestris-specific phage) was evaluated combination with 6-pentyl-α-pyrone (a secondary metabolite produced by Trichoderma atroviride P1) mineral hydroxyapatite. Then, antibiofilm interaction, called a φHA6PP complex, investigated using...
Abstract The Toll-interleukin 1 receptor superfamily includes the genes interleukin receptor-like ( IL1RL1 ), Toll like receptors TLRs myeloid differentiation primary-response 88 MyD88 and adaptor-like TIRAP ). This study describes interaction between MyD88, against Helicobacter pylori infection. Cases controls were genotyped at polymorphic sites rs6853, rs8177374 rs11123923. results show that specific combinations of - (AA-CT; P: 2,8 × 10 –17 ) (AA-CT-AA; 1,4 –8 – but not alone—act...
The study describes the Salmonella Rissen phage ϕ1 isolated from ϕ1-sensitive strain RW. same was then used to select resistant RRϕ1+, which can harbour or not ϕ1. Following this approach, we found that ϕ1, upon excision RW cells with mitomycin, behaves as a temperate phage: lyses host and generates particles; instead, spontaneous RRϕ1+ cells, it does generate causes loss of resistance; switches O-antigen smooth rough phenotype, favors transition planktonic biofilm growth. strains differ by...
The identification of novel strategies to control Helicobacter pylori (Hp)-associated chronic inflammation is, at present, a considerable challenge. Here, we attempt combat this issue by modulating the innate immune response, targeting formyl peptide receptors (FPRs), G-protein coupled that play key roles in both regulation and resolution inflammatory response. Specifically, investigated, vitro, whether Caulerpin-a bis-indole alkaloid isolated from algae genus Caulerpa-could act as molecular...
Table S1 Sequence alignment of upstream and downstream regions at position 1022. Capital letters indicate the polypirimidine-reach region. S2 Primer sequence oligonucleotides used in AS-PCR. S3 Hardy-Weinberg equilibrium based on g.1022G>A water buffalo infected not with Mycobacterium bovis. S4 Association between resistance to bovis infection polymorphism replication study group 2. Please note: The publisher is responsible for content or functionality any supporting information supplied by...
Non-typhoid Salmonella (NTS) represents one of the major causes foodborne diseases, which are made worse by increasing emergence antibiotic resistance. Thus, NTS a significant and common public health concern. The purpose this study is to investigate whether selection for phage-resistance alters bacterial phenotype, making approach suitable candidate vaccine preparation. We therefore compared two strains enterica serovar Rissen: RR (the phage-resistant strain) RW phage-sensitive in order...