Two dimensional materials beyond graphene such as MoS2 and WS2 are novel interesting class of whose unique physico-chemical properties can be exploited in applications ranging from leading edge nanoelectronics to the frontiers between biomedicine biotechnology. To unravel potential TMD crystals biomedicine, control over their production through green scalable routes biocompatible solvents is critically important. Furthermore, considering multiple eco-friendly 2D dispersions impact onto live...

In this study, we investigated the mechanism of action 10-aminoacid RiLK1 peptide against Escherichia coli (strain ATCC 25922), both in vitro and contaminated meat matrices. Therefore, a mass spectrometry-based functional proteomics platform was employed to identify specific molecular targets membrane protein-enriched E. lysate obtain information on their interaction mechanism. This target deconvolution approach combines MS-limited proteolysis techniques, like Drug Affinity Responsive Target...

Due to the rapidly increasing resistance conventional antibiotics, antimicrobial peptides are emerging as promising novel drug candidates. In this study, peptide fragments were obtained from yellowfin tuna muscle by simulated gastrointestinal digestion, and their activity towards Gram-positive Gram-negative bacteria was investigated. particular, of both medium- short-sized investigated using two dedicated approaches. Medium-sized purified solid phase extraction on C18, while short thanks a...

Soybeans (Glycine max) are an excellent source of dietary proteins and peptides with potential biological activities, such as antihypertensive, anti-cholesterol, antioxidant activity; moreover, they could prevent cancer. Also, soy contains all the essential amino acids for nutrition; therefore, it represents alternative to animal proteins. The goal this paper was comprehensive characterization medium-sized short (two four acids) obtained from simulated gastrointestinal digestion. Two...

Pseudomonas fluorescens is an opportunistic, psychotropic pathogen that can live in different environments, such as plant, soil, or water surfaces, and it associated with food spoilage. Bioactive compounds be used antimicrobials added into packaging systems. Quercetin lactoferrin are the best candidates for development of a complex two molecules absorbed on bio combability structure hydroxyapatite. The minimum inhibiting concentration (MIC) single components dropped down MIC value against...

In this work, the effect of an alginate-based coating loaded with hydroxyapatite/lactoferrin/quercetin (HA/LACTO-QUE) complexes during storage pork meat was evaluated. FT-IR spectra HA/LACTO-QUE confirmed adsorption QUE and LACTO into HA crystals showing characteristic peaks both active compounds. The kinetic releases from coatings in aqueous medium pointed out a faster release than QUE. activated showed high capability to slow down growth total viable bacterial count, psychotropic bacteria...

The epidemiology of Salmonella Infantis is complex in terms its distribution and transmission. continuous collection analysis updated data on the prevalence antimicrobic resistance are essential. present work aimed to investigate antimicrobial correlation among S. isolates from different sources through multiple-locus variable-number tandem repeat (VNTR) (MLVA). A total 562 strains isolated 2018 2020 poultry, humans, swine, water buffalo, mussels, cattle, wild boar were serotyped, 185...

Helicobacter pylori ( H . ) is a common human pathogen causing inflammation. Recent studies have suggested sophisticated interplay between mitochondria, innate immunity and inflammatory response, thus proposing mitochondrial disfunction as the hallmark of severe disorders. In this study, humic substances isolated from composted fennel residues (HS-FEN) were tested potential therapeutical strategy to restore physiology control inflammation associated with infection. The molecular features...

Salmonella is one of the most common agents foodborne illness. The genus includes two species ( bongori and S. enterica ) six subspecies I, salamae II, arizonae IIIa, diarizonae IIIb, houtenae IV, indica VI), each which contains multiple serotypes associated with animal human infections. aim study was to evaluate presence spp. in carcasses food-producing animals foods southern Italy serovar distribution among different sources. From 2011 2021, a total 12,246 982 samples from were collected...

Short peptides have been spiking interest owing to their significant contribution the taste and functional properties of dry-cured ham. In this study, a suspect screening approach based on high-resolution mass spectrometry was employed for comprehensive characterization short endogenous peptidome in ham samples at different processing stages (14, 22, 34 months). After careful manual spectra interpretation, chemometric principal component analysis highlighting differences between three sets...

Bacillus cereus is a spoilage bacterium and recognized as an agent of food poisoning. Two food-borne illnesses are caused by B. cereus: diarrheal disease, associated with cytotoxin K, hemolysin BL, non-hemolytic enterotoxin FM, emetic syndrome, the cereulide toxin. Owing to heat resistance its ability grow in milk, this organism should be considered potentially hazardous dairy products. The present study assessed risk poisoning due consumption water buffalo mozzarella cheese. A total 340...

Summary In this study, the effect of alginate‐based coatings charged with quercetin glycoside compounds and hydroxyapatite/quercetin (HA/QUE) on microbiological quality, bioactive fresh‐cut papaya, was evaluated for 14 days at 6 °C. Alginate showed a high capability to slow down growth all parameters investigated. At end cold storage, total bacteria count in papaya samples covered HA/QUE alginate coating 4.8 log CFU g −1 which is significantly lower ( P < 0.05) than 8.3 uncoated samples....

In this work, active alginate-based coatings were developed using hydroxyapatite nanoparticles as potential carriers for quercetin glycoside compounds. The produced through the layer-by-layer method and loaded with different concentrations of hydroxyapatite/quercetin complexes. In-vitro release studies performed in an aqueous medium: even if nanocrystals slow down diffusion process, released reached equilibrium one day all coatings. Lastly, antimicrobial tests show that display antibacterial...

The identification of novel strategies to control Helicobacter pylori (Hp)-associated chronic inflammation is, at present, a considerable challenge. Here, we attempt combat this issue by modulating the innate immune response, targeting formyl peptide receptors (FPRs), G-protein coupled that play key roles in both regulation and resolution inflammatory response. Specifically, investigated, vitro, whether Caulerpin-a bis-indole alkaloid isolated from algae genus Caulerpa-could act as molecular...

Fresh ricotta (whey cheese), owing to its technological characteristics and intrinsic parameters (pH a<sub>w</sub>), is an excellent substrate for the growth of spoilage pathogenic microorganisms. On 19 July 2010, Campania buffalo cheese was assigned register Protected Designations Origin (PDO) by European Commission, with EC Reg. 634/2010. It mainly produced in provinces Caserta Salerno around rivers Garigliano Volturno, plain river Sele area Cilento. This article reports...

Non-typhoid Salmonella (NTS) represents one of the major causes foodborne diseases, which are made worse by increasing emergence antibiotic resistance. Thus, NTS a significant and common public health concern. The purpose this study is to investigate whether selection for phage-resistance alters bacterial phenotype, making approach suitable candidate vaccine preparation. We therefore compared two strains enterica serovar Rissen: RR (the phage-resistant strain) RW phage-sensitive in order...

Food safety is a fundamental requirement in mass catering, as large numbers of meals are served each day to potentially vulnerable consumers, such children. Business Operators implement plans for the microbiological monitoring prepared and catering sector, swab-sampling surfaces. From January 2018 June 2019, our laboratory analyzed both food swab samples from four facilities. Considering EFSA data, we specifically focused on Bacillus cereus. Our data substantially showed episodic...

Rennet is a critical element that allows the coagulation of milk caseins, basic process producing cheese. Traditional cheeses (Traditional Agri-food Products - PAT) are generally produced using artisanal animal rennet. The type rennet used for cheese making, jointly with several other factors, influences flavor and aroma dairy products. In this study, we focused exclusively on rennet’s role in developing aromas flavors, highlighting different tastes depending cheesemaking. Buffalo was to...

Abstract Helicobacter pylori ( H. ) is a common human pathogen causing inflammation. Recent studies have suggested sophisticated interplay between mitochondria, innate immunity and inflammatory response, thus proposing mitochondrial disfunction as the hallmark of severe disorders. In this study, humic substances isolated from composted fennel residues (HS-FEN) were tested potential therapeutical strategy to restore physiology control inflammation associated with infection. The molecular...