Abstract Environmental pollutants from different chemical families may reach the gut microbiome, where they can be metabolized and transformed. However, how our symbionts respond to exposure environmental pollution is still underexplored. In this observational, cohort study, we aim investigate influence of on microbiome composition potential activity by shotgun metagenomics. We select as a case study population living in highly polluted area Campania region (Southern Italy), proposed an...

Bovine papillomavirus type 2 (BPV-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the Marmara Black Sea Regions Turkey. BPV-2 DNA found both neoplastic non-neoplastic lesions bladder. Furthermore, this virus may be normal inhabitant since also detected clinically buffaloes. The viral activation by fern immunosuppressant or carcinogen trigger urothelial cell transformation. E5 oncoprotein was solely tumours appeared...

Papillomaviruses (PVs) are believed to be highly epitheliotropic as they usually establish productive infections within stratified epithelia. In vitro, various PVs appear complete their entire life-cycle in different trophoblastic cell lines. this study, infection by and protein expression of bovine papillomavirus type 2 (BPV-2) the uterine chorionic epithelium placenta has been described four cows suffering from naturally occurring papillomavirus-associated urothelial bladder tumors. E5...

Bovine papillomavirus type 13 (BPV-13), a novel Deltapapillomavirus, has been found associated with urothelial tumours of the urinary bladder cattle grazing on lands infested bracken fern. BPV-13 was detected in 28 39 tumours. Diagnosis based sequencing L1 and E5 amplicons from tumour samples. The nucleotide sequences generated these showed 100% homology DNA Brazil fibropapilloma ear cow equine sarcoids two horses. GenBank accession number our representative is JQ798171.1. Furthermore, mRNA...

Background Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, anogenital tract and oral cavity. In this study, early (E) late (L) protein expression bovine papillomavirus type 2 (BPV-2) in urothelium urinary bladder is described cows water buffaloes suffering from naturally occurring papillomavirus-associated urothelial tumors. Methods Findings E5 protein, major oncoprotein BPV-2, was detected all L1 DNA...

Microscopic patterns of thirty-four urothelial tumors the urinary bladder water buffaloes from Marmara and Black Sea Regions Turkey are here described. All animals grazed on lands rich in bracken fern. Histological diagnosis was assessed using morphological parameters recently suggested for cattle. Papillary carcinoma most common neoplastic lesion (22/34) observed this study, low-grade more (seventeen cases) than high-grade (five cases). Papilloma, papillary neoplasm low malignant potential...

Otitis externa is a common multifactorial disease in dogs, characterized by broad and complex modifications of the ear microbiota. The goal our study was to describe cerumen microbiota healthy within same animal between different animals, compare otitis affected dogs with that animals. present included 26 16 animals bilateral 4 monolateral externa. For each samples from right left were separately collected sterile swabs, processed for DNA extraction PCR amplification 16S rRNA gene. Amplicon...

Impaired fertility associated with disorders of sex development (DSDs) due to genetic causes in dogs are more and frequently reported. Affected usually specific breeds thus representing a cause economic losses for breeders. The aim this research is report the clinical, cytogenetic molecular findings four XX SRY-negative DSD dog cases. All subjects showed female aspect presence an enlarged clitoris penis bone. Morphopathological analyses performed three cases testes two ovotestis another....

The protein MucR from Brucella abortus has been described as a transcriptional regulator of many virulence genes. It is member the Ros/MucR family comprising proteins that control expression genes important for successful interaction α-proteobacteria with their eukaryotic hosts. Despite clear evidence role in repressing genes, no study carried out so far demonstrating direct this promoter its target gene babR encoding LuxR-like virB In study, we show first time ability to bind...

Active infection by bovine papillomavirus type 2 (BPV-2) was documented for fifteen urinary bladder tumors in cattle. Two were diagnosed as papillary urothelial neoplasm of low malignant potential (PUNLMP), nine and four invasive cancers.In all cancer samples, PCR analysis revealed a BPV-2-specific 503 bp DNA fragment. E5 protein, the major oncoprotein virus, shown both immunoprecipitation immunohistochemical analysis. found to bind activated (phosphorylated) form platelet derived growth...

An outbreak of winter dysentery, complicated by severe respiratory syndrome, occurred in January 2020 a high production dairy cow herd located hilly area the Calabria region. Of 52 animals belonging to farm, 5 (9.6%) died with distress, death occurring 3–4 days after appearance signs (caught and gasping breath). Microbiological analysis revealed absence pathogenic bacteria whilst Real-time PCR identified presence RNA from Bovine Coronavirus (BCoV) several organs: lungs, small intestine...

ABSTRACT Three bacteriophages, 118970_sal1, 118970_sal2, and 64795_sal3, were isolated from water buffalo feces in southern Italy, exhibiting lytic activity against Salmonella enterica serovar Enteritidis. These bacteriophages belong to the Siphoviridae family have a 60,113-bp, 123,930-bp, 48,094-bp double-stranded DNA (dsDNA) genome containing 72, 173, 80 coding sequences (CDSs), respectively.

Following the increase of wild boar (Sus scrofa) populations in Europe, a potential risk emerging infections by vector-borne pathogens may occur. Despite this, circulation piroplasmid species these ungulates is still neglected topic, particularly Mediterranean basin. Therefore, this study aimed to investigate presence Babesia/Theileria spp. boars from southern Italy assess epidemiological role piroplasmids.By using citizen science approach among hunters and veterinarians, spleen samples were...

ABSTRACT Brucellosis is a zoonotic disease caused by bacteria of the genus Brucella . The endemic in many areas, causing chronic infections responsible for reproductive disorders infected animals. Here, we present eight complete genome assemblies abortus strains isolated from water buffaloes farmed Campania region.

ABSTRACT The bacteriophage 100268_sal2 was isolated from water buffalo feces in southern Italy, exhibiting lytic activity against several subspecies of Salmonella enterica . This belongs to the Siphoviridae family and has a 125,114-bp double-stranded DNA (ds-DNA) genome containing 188 coding sequences (CDSs).

A case of Mycobacterium tuberculosis infection is described in a dead adult male dog Southern Italy. The carcass was found by the Health Authority gypsy encampment. It admitted to our forensic veterinary medicine unit, with suspicion cruelty animal. Necropsy showed beating and traumatism signs, mistreating confirmed. Gross lesions included multiple nodular hepatic lesions, hemorrhagic enteritis enlarged mesenteric lymph nodes, body cavity effusions, an adrenal neoplasm. Bacteriological...

Salmonella enterica subsp. Serovar Enteritidis is one of the major pathogens associated with enteric diseases in animals and humans. Thus, due to importance spp. infections for animal production public health, aim present study was describe first detection S. enteritidis an aborted water buffalo fetus southern Italy by characterizing phylogroup profile antimicrobial susceptibility isolated pathogenic strains. The different clinical manifestations salmonellosis include diarrhea, abortion,...

Listeria monocytogenes is a Gram-positive pathogen causing life-threatening infections both in humans and animals. In livestock farms, it can persist for long time primarily causes uterine encephalitis farmed Whole genome sequencing (WGS) currently becoming the best method molecular typing of this due to its high discriminatory power efficiency characterization. This study describes WGS-based characterization an L. strain from aborted water buffalo fetus southern Italy. The under was...