A5066626188- SARS-CoV-2 and COVID-19 Research
- RNA regulation and disease
- SARS-CoV-2 detection and testing
- RNA Research and Splicing
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Complement system in diseases
- RNA modifications and cancer
- Air Quality and Health Impacts
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune responses and vaccinations
- interferon and immune responses
- Zoonotic diseases and public health
- Dental Research and COVID-19
- Fibroblast Growth Factor Research
- Biosensors and Analytical Detection
- PARP inhibition in cancer therapy
- Cancer, Hypoxia, and Metabolism
- Genomics and Chromatin Dynamics
- Phagocytosis and Immune Regulation
- GABA and Rice Research
- COVID-19 diagnosis using AI
- Health, Environment, Cognitive Aging
- Infection Control and Ventilation
- Cytokine Signaling Pathways and Interactions
Istituto Zooprofilattico Sperimentale del Mezzogiorno
2020-2023
University of Salerno
2017-2022
The established risk factors of coronavirus disease 2019 (COVID-19) are advanced age, male sex, and comorbidities, but they do not fully explain the wide spectrum manifestations. Genetic implicated in host antiviral response provide for novel insights into its pathogenesis.We performed an in-depth genetic analysis chromosome 21 exploiting genome-wide association study data, including 6,406 individuals hospitalized COVID-19 902,088 controls with European ancestry from Host Genetics...
Abstract Environmental pollutants from different chemical families may reach the gut microbiome, where they can be metabolized and transformed. However, how our symbionts respond to exposure environmental pollution is still underexplored. In this observational, cohort study, we aim investigate influence of on microbiome composition potential activity by shotgun metagenomics. We select as a case study population living in highly polluted area Campania region (Southern Italy), proposed an...
The RNA-binding protein Argonaute 2 (AGO2) is a key effector of RNA-silencing pathways It exerts pivotal role in microRNA maturation and activity can modulate chromatin remodeling, transcriptional gene regulation RNA splicing. Estrogen receptor beta (ERβ) endowed with oncosuppressive activities, antagonizing hormone-induced carcinogenesis inhibiting growth oncogenic functions luminal-like breast cancers (BCs), where its expression correlates better prognosis the disease.Applying interaction...
Long-chain polyphosphates inhibit SARS-CoV-2 infection by targeting a host receptor and viral RNA polymerase.
Abstract Background Genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the only approach to rapidly monitor and tackle emerging variants concern (VOC) COVID-19 pandemic. Such scrutiny crucial limit spread VOC that might escape immune protection conferred by vaccination strategies or previous virus exposure. It also becoming clear now efficient genomic would require monitoring host gene expression identify prognostic biomarkers treatment efficacy disease...
Colorectal cancer (CRC) is a high incidence disease, characterized by morbidity and mortality rates. Early diagnosis remains challenging because fecal occult blood screening tests have performed sub-optimally, especially due to hemorrhoidal, inflammatory, vascular diseases, while colonoscopy invasive requires medical setting be performed. The objective of the present study was determine if serum metabolomic profiles could used develop novel approach for colorectal cancer. Furthermore,...
Genome-wide association studies (GWAS) found locus 3p21.31 associated with severe COVID-19. CCR5 resides at the same and, given its known biological role in other infection diseases, we investigated if common noncoding and rare coding variants, affecting CCR5, can predispose to We combined single nucleotide polymorphisms (SNPs) that met suggestive significance level (P ≤ 1 × 10-5) public GWAS datasets (6406 COVID-19 hospitalized patients 902,088 controls) gene expression data from 208 lung...
Bivalve shellfish are readily contaminated by human pathogens present in waters impacted municipal sewage, and the detection of SARS-CoV-2 feces infected patients wastewater has drawn attention to possible presence virus bivalves. The aim this study was collect data on prevalence bivalve mollusks from harvesting areas Campania region. A total 179 samples were collected between September 2019 April 2021 tested using droplet digital RT-PCR (dd RT-PCR) real-time RT-PCR. Combining results...
SARS-CoV-2, the causative agent of COVID-19 pandemic, has rarely been associated with transmission from humans to animals (reverse zoonotic transmission). In this retrospective study, authors reviewed data obtained 236 animals, including buffaloes, goats/sheep, horses, carrier pigeons, rabbits, hens, snakes, pigs and cows that were screened for SARS-CoV-2 infection because they had in contact their SARS-CoV-2-positive breeder at least 2 weeks. None tested found be positive. The authors'...
To identify host genetic determinants involved in humoral immunity and associated with the risk of developing severe COVID-19, we analyzed 500 SARS-CoV-2 positive subjects from Southern Italy. We examined coding sequences 10 common variable immunodeficiency-associated genes obtained by whole-exome sequencing 121 hospitalized patients. These showed significant enrichment predicted pathogenic point mutations patients compared non-severe ones. Moreover, TNFRSF13C gene, minor allele p.His159Tyr...
SARS-CoV-2 can be detected in the feces of infected people, consequently wastewater, and bivalve mollusks, that are able to accumulate viruses due their ability filter large amounts water. This study aimed monitor RNA presence 168 raw wastewater samples collected from six treatment plants (WWTPs) 57 mollusk obtained eight harvesting sites Campania, Italy. The monitoring period spanned October 2021 April 2022, results were compared correlated with epidemiological situation. In sewage, ORF1b...
The Italian municipality of Ariano Irpino (Avellino, Campania, Italy) was locked down by the regional authorities from March until April 2020 after several citizens tested positive for SARS coronavirus 2 (SARS-CoV-2). A serological mass screening campaign targeting population using Roche Cobas Elecsys anti-SARS-CoV-2 assay organized Zoo-Prophylactic Institute Southern Italy (Portici, and conducted in cooperation with Local Health Unit (Azienda Sanitaria Locale – ASL Avellino, Italy),...
Emerging evidence suggest that infection-dependent hyperactivation of complement system (CS) may worsen COVID-19 outcome. We investigated the role predicted high impact rare variants - referred as qualifying (QVs) CS genes in predisposing asymptomatic elderly individuals, known to be more susceptible severe disease.Exploiting exome sequencing data and 56 genes, we performed a gene-based collapsing test between 164 subjects (aged ≥60 years) 56,885 European individuals from Genome Aggregation...
The SARS-CoV-2 can spread directly via saliva, respiratory aerosols and droplets, indirectly by contact through contaminated objects and/or surfaces air. In the context of COVID-19 fomites be an important vehicle virus transmission contribute to infection risk in public environments. aim study was analyze surface sampling (sponge method) presence working environments, order evaluate for transmission. Seventy-seven environmental samples were taken using sterile sponges 17 animal farms, 4...
The development of prophylactic agents against the SARS-CoV-2 virus is a public health priority in search for new surrogate markers active replication. Early detection are needed to follow disease progression and foresee patient negativization. Subgenomic RNA transcripts (with focus on sgN) were evaluated oro/nasopharyngeal swabs from COVID-19-affected patients with an analysis 315 positive samples using qPCR technology. Cut-off Cq values sgN (Cq < 33.15) sgE 34.06) showed correlations high...
The Experimental Zooprophylactic Institute of Southern Italy (Istitituto Zooprofilattico Sperimentale del Mezzogiorno, IZSM) is a public health institution operating within the Italian National Health Service. Over past 5 years [IZSM] has promoted several research studies and interventions in an effort to tackle 'Land Fires' phenomenon, caused by continued trafficking uncontrolled incineration waste that affected some areas Campania for decades. In this article, mathematical model generates...
The aim of the present study is to assess saliva as a reliable specimen for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection by real-time reverse transcription-PCR (RT-PCR), especially in community mass screening programs. performance analysis considered 1,221 total samples [nasopharyngeal (NP) swabs and corresponding saliva], tested means reference diagnostic RT-PCR assay. Conflicting results were further investigated with second, more sensitive, Analysis agreement...
As of November 17, 2021, SARS-CoV-2 (Severe Acute Respiratory Syndrome CoronaVirus 2), the causative agent COVID-19 (COronaVIrus Disease 19), has infected ~250 million people worldwide, causing around five deaths. Titers anti-SARS-CoV-2 neutralizing antibodies were relatively stable for at least 9 months in a population-based study conducted Wuhan, China, both symptomatic and asymptomatic individuals. In mass screening campaign town Ariano Irpino (Avellino, Italy) May, 2020, 5.7% (95% CI:...
Given the unequivocal relationship between levels of environmental contamination and results HBM studies, our three-step approach for geographical stratification definition sample size in regional surveys is based on use a synthetic indicator pressure computed at municipality level, namely index (MEPI).
Abstract Anti-viral activities of long-chain inorganic polyphosphates (PolyPs) against severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infection were investigated. In molecular docking analyses, PolyPs interacted with several conserved angiotensin-converting enzyme (ACE)2 and RNA-dependent RNA polymerase (RdRp) amino acids. We thus tested for functional interactions in vitro SARS-CoV-2–infected Vero E6, Caco2 human primary nasal epithelial cells. Immunofluorescence, qPCR, direct...
In this retrospective study, a cohort of 67 subjects vaccinated with AZD1222 was retrospectively observed. Consistently published findings, no serious adverse event reported, and all events reported (fever, muscle ache and/or pain in the site injection) had resolved by day 8. Of note, some citizens were prescribed low-dose aspirin even heparin for thrombosis prevention. We also found variations laboratory test results (full blood count chemistry) on 1 compared Physicians should be aware that...
Abstract The COVID-19 disease, caused by the SARS-Cov-2, presents a heterogeneous clinical spectrum. risk factors do not fully explain wide spectrum of disease manifestations, so it is possible that genetic could account for novel insights into its pathogenesis. In our previous study, we hypothesized common variants on chromosome 21, near TMPRSS2 and MX1 genes, may be associated to different manifestations COVID-19. Here, performed an in-depth analysis 21 exploiting genome-wide association...
In response to the complex social, environmental and economic situation caused by "Terra dei Fuochi" phenomenon, IZSM collaborated closely with Terra Fuochi working group, carrying out additional monitoring surveys both on food (QR Code Campania project[30]), environment (Campania Trasparente project[31]). This model was developed in context of experience accumulated field monitoring, represents an innovative tool aimed at increasing knowledge Region through objective, integrated organic...