A5041732138- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Biochemical and Structural Characterization
- Neonatal Respiratory Health Research
- Aquaculture disease management and microbiota
- Pediatric health and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Invertebrate Immune Response Mechanisms
- Essential Oils and Antimicrobial Activity
- Animal Nutrition and Physiology
- RNA Interference and Gene Delivery
- Probiotics and Fermented Foods
- Animal Virus Infections Studies
- Protein Hydrolysis and Bioactive Peptides
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Lipid Membrane Structure and Behavior
- Complement system in diseases
- Bacterial biofilms and quorum sensing
- Immunotherapy and Immune Responses
- Antibiotic Resistance in Bacteria
- Listeria monocytogenes in Food Safety
- Neuroscience of respiration and sleep
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
Utrecht University
2016-2025
Southwest University
2022
University of British Columbia
2005
The formation of biofilm by bacteria confers resistance to biocides and presents problems in medical veterinary clinical settings. Here we report the effect carvacrol, one major antimicrobial components oregano oil, on biofilms its activity existing biofilms. Assays were carried out polystyrene microplates observe (a) 0–0.8 mM carvacrol selected bacterial pathogens over 24 h (b) 0–8 stability pre-formed Carvacrol was able inhibit Chromobacterium violaceum ATCC 12472, Salmonella enterica...
The common avian origin of many zoonotic infections and epidemics warrants investigation into the mechanism respiratory surface protection in reservoir species such as birds. Our recent molecular investigations on evolution pulmonary expression an ancient family proteins, C-type lectins, have revealed unique adaptations surfactant proteins SP-A1 (aSP-A1), aSP-A2 aSP-C coupled with loss protein-D (SP-D) lineage. As are members collectin family, a subgroup silico search for related...
Carvacrol is a component of several essential oils and has been shown to exert antimicrobial activity. The structural requirements for the activity carvacrol were determined by comparison structurally related (nonessential oil) compounds. Removal aliphatic ring substituents slightly decreased effect hydroxyl group on could not be simply comparing it p-cymene, because this compound immiscible with water; therefore, 2-amino-p-cymene, amino analogue carvacrol, which similar hydrophobicity...
ABSTRACT The essential oils of oregano and thyme are active against a number food-borne pathogens, such as Escherichia coli O157:H7. Carvacrol is one the major antibacterial components these oils, p -cymene thought to be its precursor in plant. effects carvacrol on protein synthesis E. O157:H7 ATCC 43895 cells were investigated. Bacteria grown overnight Mueller-Hinton broth with sublethal concentration or -cymene, their compositions analyzed by sodium dodecyl sulfate-polyacrylamide gel...
The porcine cathelicidin PR-39 is a host defence peptide that plays pivotal role in the innate immune of pig against infections. Besides direct antimicrobial activity, it involved immunomodulation, wound healing and several other biological processes. In this study, antimicrobial- immunomodulatory activity PR-39, N- C-terminal derivatives were tested. exhibited an unexpected broad spectrum including Gram positive strains such as Bacillus globigii Enterococcus faecalis. Of organisms tested,...
Abstract Cathelicidins are short cationic peptides initially described as antimicrobial peptides, which can also modulate the immune system. Because most findings have been in context of human LL-37 or murine CRAMP, investigated under varying conditions, it is unclear functions cathelicidin specific and general properties. This study compares 12 cathelicidins from 6 species standardized conditions to better understand conservation functions. Most tested had strong activity against E. coli...
Abstract Host defense peptides (HDPs) play a pivotal role in innate immunity and have, addition to antimicrobial activity, also important immunomodulatory functions. Bacteria are less likely develop resistance against HDPs because these target kill bacteria multiple ways, as well modulate the immune system. Therefore, HDPs, derivatives thereof, promising alternatives traditional antibiotics. Hardly anything is known about functions of porcine cathelicidin PMAP-36. In this study, we aimed...
Abstract The natural antibiotic teixobactin kills pathogenic bacteria without detectable resistance. difficult synthesis and unfavourable solubility of require modifications, yet insufficient knowledge on its binding mode impedes the hunt for superior analogues. Thus far, teixobactins are assumed to kill by cognate cell wall precursors (Lipid II III). Here we present in cellular membranes using solid-state NMR, microscopy, affinity assays. We solve structure complex formed an improved...
Food-borne pathogens are responsible for most cases of food poisoning in developed countries and often associated with poultry products, including chicken. Little is known about the role beta-defensins chicken digestive tract their efficacy. In this study, expression beta-defensin gallinacin-6 (Gal-6) its antimicrobial activity against food-borne were investigated. Reverse transcription-PCR analysis showed high Gal-6 mRNA esophagus crop, moderate glandular stomach, low throughout intestinal...
Abstract Activation of the immune system needs to be tightly regulated provide protection against infections and, at same time, prevent excessive inflammation limit collateral damage host. This tight regulation includes regulating activation TLRs, which are key players in recognition invading microbes. A group short cationic antimicrobial peptides, called cathelicidins, have previously been shown modulate TLR by synthetic or purified ligands and may play an important role during infections....
ABSTRACT Antifungal mechanisms of action two cathelicidins, chicken CATH-2 and human LL-37, were studied compared with the mode salivary peptide histatin 5 (Hst5). Candida albicans was used as a model organism for fungal pathogens. Analysis by live-cell imaging showed that peptides kill C. rapidly. is most active kills within min. Both cathelicidins induce cell membrane permeabilization simultaneous vacuolar expansion. Minimal fungicidal concentrations (MFC) are in same order magnitude all...
Abstract P . aeruginosa is a notorious biofilm producer that causes wide variety of acute and chronic infections. In this study the in vitro anti-biofilm activity 13 Host Defence Peptides from different species was tested against biofilms. Most HDPs were able to prevent attachment, due their antimicrobial effect on planktonic bacteria starting inoculum. Activity pre-formed biofilms also observed, although mainly at short incubation times. Several kill (colony counting associated bacteria)...
Abstract Host defence peptides (HDPs) have the potential to become alternatives conventional antibiotics in human and veterinary medicine. The HDP chicken cathelicidin-2 (CATH-2) has immunomodulatory direct killing activities at micromolar concentrations. In this study mechanism of action CATH-2 against Escherichia coli ( E. ) was investigated great detail using a unique combination imaging biophysical techniques. Live-imaging with confocal fluorescence microscopy demonstrated that...
Host Defence Peptides and derived peptides are promising classes of antimicrobial immunomodulatory lead compounds. For this purpose we examined whether chicken cathelicidin-2 (CATH-2)-derived modulate the function inflammatory response avian immune cells. Using a macrophage cell line (HD11) found that full-length CATH-2 dose-dependently induced transcription chemokines CXCLi2/IL-8, MCP-3 CCLi4/RANTES, but not pro-inflammatory cytokine IL-1β. In addition, efficiently inhibited IL-1β nitric...