A5085849816- Osteoarthritis Treatment and Mechanisms
- Inflammatory mediators and NSAID effects
- Polyamine Metabolism and Applications
- NF-κB Signaling Pathways
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Chemokine receptors and signaling
- Proteoglycans and glycosaminoglycans research
- Autophagy in Disease and Therapy
- Cytokine Signaling Pathways and Interactions
- Cancer-related molecular mechanisms research
- Immune Response and Inflammation
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Peroxisome Proliferator-Activated Receptors
- Antimicrobial Peptides and Activities
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Electrospun Nanofibers in Biomedical Applications
- interferon and immune responses
- Diabetes and associated disorders
- Nerve injury and regeneration
- Bone Metabolism and Diseases
- Viral Infections and Immunology Research
- Mast cells and histamine
- Mesenchymal stem cell research
Istituto Ortopedico Rizzoli
2015-2024
University of Bologna
1990-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2002-2023
Orthopedic Institute
2016
Nordic Bioscience (Denmark)
2009
Stony Brook University
2005
New York University Langone Orthopedic Hospital
2005
Istituto di Genetica Molecolare
1992
Medica (Italy)
1991
Human cartilage is a complex tissue of matrix proteins that vary in amount and orientation from superficial to deep layers loaded unloaded zones.A major challenge efforts repair by stem cell-based other engineering strategies the inability resident chondrocytes lay down new with same structural resilient properties it had upon its original formation.This particularly true for collagen network, which susceptible cleavage once proteoglycans are depleted.Thus, thorough understanding...
Abstract Introduction Recent evidence suggests that tissue accumulation of senescent p16 INK4a -positive cells during the life span would be deleterious for functions and could consequence inherent age-associated disorders. Osteoarthritis (OA) is characterized by chondrocytes expressing markers senescence-associated secretory phenotype (SASP), including matrix remodeling metalloproteases MMP1/MMP13 pro-inflammatory cytokines interleukin-8 (IL-8) IL-6. Here, we evaluated role in OA-induced...
Objective Human chondrocytes produce different C-X-C and C-C chemokines under basal conditions upon activation with proinflammatory cytokines. We investigated whether human also have chemokine receptors examined the effects of on chondrocyte activity. Methods The expression was determined by immunochemical analysis frozen sections from normal osteoarthritic cartilage flow cytometry isolated cells. messenger RNA for studied reverse transcriptase–polymerase chain reaction. Isolated were...
Abstract The therapeutic ability of Mesenchymal Stem/Stromal Cells to address osteoarthritis (OA) is mainly related the secretion biologically active factors, which can be found within their secreted Extracellular Vesicles including small (sEV). Aim this study was investigate effects sEV from adipose derived stromal cells (ADSC) on both chondrocytes and synoviocytes, in order gain insights into mechanisms modulating inflammatory/catabolic OA environment. sEV, obtained by a combined...
Background In osteoarthritis (OA), an inflammatory environment is responsible for the imbalance between anabolic and catabolic activity of chondrocytes and, thus, articular cartilage derangement. This study was aimed at providing further insight into impairment cytokine IL-4 its receptors in human OA cartilage, as well potential ability to antagonize phenotype induced by IL-1β. Methodology/Principal Findings The vivo expression receptor subunits (IL-4R, IL-2Rγ, IL-13Rα1) investigated on full...
Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment inflamed joints intracellular content CC and CXC chemokines was investigated. IL‐8, GROα, MCP‐1, RANTES, MIP‐1α MIP‐1β expression evaluated by flow cytometric analysis RT‐PCR chondrocytes isolated from cartilage specimens obtained patients with osteoarthritis rheumatoid arthritis multiorgan donors as normal controls. All except RANTES were...
Abstract Objective Osteoarthritic (OA) chondrocytes behave in an intrinsically deregulated manner, characterized by chronic loss of healthy cartilage and inappropriate differentiation to a hypertrophic‐like state. IKKα IKKβ are essential kinases that activate NF‐κB transcription factors, which turn regulate cell inflammation. This study was undertaken investigate the differential roles each IKK chondrocyte hypertrophy. Methods Expression or ablated primary human retro‐transduction specific...
To link matrix metalloproteinase 13 (MMP-13) activity and extracellular (ECM) remodeling to alterations in regulatory factors leading a disruption chondrocyte homeostasis.MMP-13 expression was ablated primary human chondrocytes by stable retrotransduction of short hairpin RNA. The effects MMP-13 knockdown on key regulators differentiation (SOX9, runt-related transcription factor 2 [RUNX-2], beta-catenin) angiogenesis (vascular endothelial growth [VEGF]) were scored at the protein level (by...
The non-canonical NF-κB activating kinase IKKα, encoded by CHUK (conserved-helix-loop-helix-ubiquitous-kinase), has been reported to modulate pro- or anti- inflammatory responses, cellular survival and differentiation. Here, we have investigated the mechanism of action IKKα as a novel effector human murine chondrocyte extracellular matrix (ECM) homeostasis differentiation towards hypertrophy.IKKα expression was ablated in primary osteoarthritic (OA) chondrocytes immature articular (iMACs)...
To extend the study of chemokine receptor repertoire on human chondrocytes to receptors with reported housekeeping functions (CXCR3, CXCR4, CXCR5, and CCR6) evaluate whether ligands these play a role in chondrocyte phenotype modulation proliferation.Chemokine expression was determined by flow cytometry. Subcultures were collected fixed at confluence or during exponential phase growth analyzed for modulation. The effects chemokines isolated cells as well cultured within an intact...
Hydroxytyrosol (HT), a phenolic compound mainly derived from olives, has been proposed as nutraceutical useful in prevention or treatment of degenerative diseases. In the present study we have evaluated ability HT to counteract appearance osteoarthritis (OA) features human chondrocytes. Pre-treatment monolayer cultures chondrocytes with was effective preventing accumulation reactive oxidant species (ROS), DNA damage and cell death induced by H2O2 exposure, well increase mRNA level...
Introduction Recent evidence suggests that GSK3 activity is chondroprotective in osteoarthritis (OA), but at the same time, its inactivation has been proposed as an anti-inflammatory therapeutic option. Here we evaluated extent of GSK3β vivo OA knee cartilage and molecular events downstream vitro to assess their contribution cell senescence hypertrophy. Methods In level phosphorylated was analyzed oxidative damage assessed by 8-oxo-deoxyguanosine staining. The effects (using either LiCl or...
Following inflammatory stimuli, GSK3 inhibition functions as a hub with pleiotropic effects leading to cartilage degradation. However, little is known about the triggered by its direct well on mitochondrial pathology, that contributes osteoarthritis pathogenesis. To this aim we assessed molecular mechanisms GSK3β inactivating stimuli 3-D (micromass) cultures of human articular chondrocytes. Stimuli were delivered either at micromass seeding (long term) or after maturation (short explore...
Abstract NF‐κB transcription factors induce a host of genes involved in pro‐inflammatory/stress‐like responses; but the collateral effects and consequences sustained activation on other cellular gene expression programming remain less well understood. Here enforced constitutively active IKKβ T‐loop mutant (IKKβca) drove murine fibroblasts into transient growth arrest that subsided within 2–3 weeks continuous culture. Proliferation was associated with G1/S phase block immortalized primary...