Francesco Ravaioli
A5085261095- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Parkinson's Disease Mechanisms and Treatments
- Birth, Development, and Health
- RNA modifications and cancer
- MicroRNA in disease regulation
- Identity, Memory, and Therapy
- Restless Legs Syndrome Research
- Immune responses and vaccinations
- Down syndrome and intellectual disability research
- Genetics, Aging, and Longevity in Model Organisms
- Family Support in Illness
- Dietary Effects on Health
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Cancer-related gene regulation
- Neuroscience and Neural Engineering
- Alzheimer's disease research and treatments
- Telomeres, Telomerase, and Senescence
- 3D Printing in Biomedical Research
- Genomics and Chromatin Dynamics
- Neurological disorders and treatments
- Diabetes and associated disorders
- Planarian Biology and Electrostimulation
Istituto delle Scienze Neurologiche di Bologna
2023-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2023-2024
University of Bologna
2017-2023
Abstract Epigenetic remodeling is one of the major features aging process. We recently demonstrated that DNA methylation ELOVL2 and FHL2 CpG islands highly correlated with age in whole blood. Here we investigated several aspects age-associated hypermethylation FHL2. showed significantly different primary dermal fibroblast cultures from donors ages. Using epigenomic data public resources, most tissues show age. Interestingly, was not found very low replication rate. associated vitro cell...
The aim of the present study was to provide a comprehensive characterization whole genome DNA methylation patterns in replicative and ionizing irradiation- or doxorubicin-induced premature senescence, exhaustively exploring epigenetic modifications three different human cell types: somatic diploid skin fibroblasts bone marrow- adipose-derived mesenchymal stem cells. With CpG-wise differential analysis, signatures were identified: (a) type- treatment-specific signature; (b) type-specific...
The feasibility of liver transplantation from old healthy donors suggests that this organ is able to preserve its functionality during aging. To explore the biological basis phenomenon, we characterized epigenetic profile biopsies collected 45 ranging 13 90 years using Infinium HumanMethylation450 BeadChip. analysis indicates a large remodeling in DNA methylation patterns occurs, with 8,823 age-associated differentially methylated CpG probes. Notably, these changes tended level off after age...
Abstract Advanced age represents one of the major risk factors for Parkinson’s Disease. Recent biomedical studies posit a role microRNAs, also known to be remodelled during ageing. However, relationship between microRNA remodelling and ageing in Disease, has not been fully elucidated. Therefore, aim present study is unravel relevance microRNAs as biomarkers Disease within framework. We employed Next Generation Sequencing profile serum from samples informative (recently diagnosed, drug-naïve)...
Epigenetic clocks were initially developed to track chronological age, but accumulating evidence indicates that they can also predict biological age. They are usually based on the analysis of DNA methylation by genome-wide methods, targeted approaches, assessment a small number CpG sites, advisable in several settings. In this study, we epigenetic clock purposely optimized for measurement The includes six genomic regions mapping ELOVL2, NHLRC1, AIM2, EDARADD, SIRT7 and TFAP2E genes, selected...
Abstract Parkinson’s disease (PD) is the neurological disorder showing greatest rise in prevalence from 1990 to 2016. Despite clinical definition criteria and a tremendous effort develop objective biomarkers, precise diagnosis of PD still unavailable at early stage. In recent years, an increasing number studies have used omic methods unveil molecular basis PD, providing detailed characterization potentially pathological alterations various biological specimens. Metabolomics could provide...
Isolated REM Sleep Behavior Disorder (iRBD) is the strongest prodromal marker for α-synucleinopathies. Overt α-synucleinopathies and aging share several mechanisms, but this relationship has been poorly investigated in phases. Using DNA methylation-based epigenetic clocks, we measured biological videopolysomnography confirmed iRBD patients, videopolysomnography-negative population-based controls. We found that iRBDs tended to be epigenetically older than controls, suggesting accelerated...
Urea cycle disorders are enzymopathies resulting from inherited deficiencies in any genes of the cycle. In severe cases, currently available therapies marginally effective, with liver transplantation being only definitive treatment. Donor availability can limit even this therapy. Identification novel therapeutics for genetic-based diseases requires models that provide measurable hepatic functions and phenotypes. Advances stem cell genome editing technologies could investigation cell-based...
Control of ribosome biogenesis is a critical aspect the regulation cell metabolism. As ribosomal genes (rDNA) are organized in repeated clusters on chromosomes 13, 14, 15, 21, and 22, trisomy chromosome 21 confers an excess rDNA copies to persons with Down syndrome (DS). Previous studies showed alteration children DS, but epigenetic has not been investigated adults DS so far. In this study, we used targeted deep-sequencing approach measure DNA methylation (DNAm) units whole blood from 69 95...
Down syndrome (DS) is a segmental progeroid genetic disorder associated with multi-systemic precocious aging phenotypes, which are particularly evident in the immune and nervous systems. Accordingly, people DS show an increased biological age as measured by epigenetic clocks. The Ts65Dn trisomic mouse, harbors extra-numerary copies of chromosome 21 (Hsa21)-syntenic regions, was shown to recapitulate several features DS, but no biomarkers have been applied it so far. In this pilot study, we...
A growing amount of evidence suggests that the downregulation protein synthesis is an adaptive response during physiological aging, which positively contributes to longevity and can be modulated by nutritional interventions like caloric restriction (CR). The expression ribosomal RNA (rRNA) one main determinants translational rate, epigenetic modifications finely contribute its regulation. Previous reports suggest hypermethylation DNA (rDNA) locus occurs with although some species- tissue-...
Advanced age is the major risk factor for idiopathic Parkinson's disease (PD), but to date biological relationship between PD and ageing remains elusive. Here we describe rationale design of H2020 funded project "PROPAG-AGEING", whose aim characterize contribution process development. We summarize current evidences that support existence a continuum justify use Geroscience approach study PD. focus in particular on role inflammaging, chronic, low-grade inflammation characteristic elderly...
A large body of evidence indicates that environmental agents can induce alterations in DNA methylation (DNAm) profiles. Radiofrequency electromagnetic fields (RF-EMFs) are radiations emitted by everyday devices, which have been classified as "possibly carcinogenic"; however, their biological effects unclear. As aberrant DNAm genomic repetitive elements (REs) may promote instability, here, we sought to determine whether exposure RF-EMFs could affect different classes REs, such long...
The control of non-coding repeated DNA by methylation plays an important role in genomic stability, contributing to health and healthy aging. Mind-body practices can elicit psychophysical wellbeing via epigenetic mechanisms, including methylation. However, this context the effects movement meditations have rarely been examined. Consequently, current study investigates a specifically structured meditation, called Quadrato Motor Training (QMT) on level sequences. An 8-week daily QMT program...
Induced pluripotent stem cell (iPSC)-technology is an important platform in medicine and disease modeling. Physiological degeneration onset are common occurrences the aging population. iPSCs could offer regenerative medical options for age-related elderly. However, reprogramming somatic cells from elderly inefficient when successful at all. Perhaps due to their low rates of replication culture, traditional transduction approaches with centenarian fibroblasts met little success. A simple...
A large body of evidence indicates that environmental agents can induce alterations in DNA methylation (DNAm) profiles. Radiofrequency electromagnetic fields (RF-EMFs) are radiations emitted by everyday devices which have been classified as “possibly carcinogenic”, however their biological effects unclear. As aberrant DNAm genomic repetitive elements (RE) may promote instability, here we sought to determine whether exposure RF-EMFs could affect different classes RE, such long interspersed...
Abstract In humans, females live longer than males but experience a worse longevity, as genome-wide autosomal DNA methylation differences between and have been reported. So far, few studies investigated if is differently affected by aging in females. We performed meta-analysis of 4 large whole blood datasets, comparing aspects epigenetic age-dependent remodeling the two sexes: differential methylation, variability, epimutations entropy. reported that fraction (43%) sex-associated probes...