A5049546820- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Dental Health and Care Utilization
- Mycobacterium research and diagnosis
- Colorectal Cancer Screening and Detection
- Tryptophan and brain disorders
- Probiotics and Fermented Foods
- Music Therapy and Health
- Reproductive tract infections research
- Gastrointestinal motility and disorders
- Sleep and related disorders
- Genomics and Phylogenetic Studies
- Cervical Cancer and HPV Research
- Pancreatic and Hepatic Oncology Research
Universitat Politècnica de Catalunya
2020-2025
Barcelona Supercomputing Center
2020-2025
Institute for Research in Biomedicine
2020-2025
Institució Catalana de Recerca i Estudis Avançats
2024
Institute for Research in Biomedicine
2023
Abstract Background Gut dysbiosis has been associated with colorectal cancer (CRC), the third most prevalent in world. This study compares microbiota taxonomic and abundance results obtained by 16S rRNA gene sequencing (16S) whole shotgun metagenomic to investigate their reliability for bacteria profiling. The experimental design included 156 human stool samples from healthy controls, advanced (high-risk) lesion patients (HRL), CRC cases, each sample sequenced using both methods. We...
Digestive disorders of gut-brain interaction (DGBI) are very common, predominant in females, and usually associated with intestinal barrier dysfunction, dysbiosis, stress. We previously found that females have increased susceptibility to dysfunction response acute However, whether this is changes the small bowel microbiota remains unknown. evaluated stress better understand stress-induced dysfunction. Jejunal biopsies were obtained at baseline 90 min after cold pain or sham Autonomic (blood...
Colorectal cancer (CRC) is the third most common and second leading cause of deaths worldwide. Early diagnosis CRC, which saves lives enables better outcomes, generally implemented through a two-step population screening approach based on use Fecal Immunochemical Test (FIT) followed by colonoscopy if test positive. However, FIT step has high false positive rate, there need for new predictive biomarkers to prioritize cases colonoscopy. Here we used 16S rRNA metabarcoding from samples uncover...
Abstract When collecting oral and fecal samples for large epidemiological microbiome studies, optimal storage conditions such as immediate freezing, are not always feasible. It is fundamental to study the impact of temporary room temperature (RT) shipping on diversity obtained in different types samples. We performed a pilot aimed at validating sampling protocol based viability 16S rRNA gene sequencing Fecal from five participants were collected preserved conditions: a) 70% ethanol; b) FIT...
Heart failure (HF) is a common condition associated with high rate of hospitalizations and adverse outcomes. HF characterized by impairments either the cardiac ventricular filling, ejection blood capacity or both. Sleep fragmentation (SF) involves series short sleep interruptions that lead to fatigue contribute cognitive dementia. Both conditions are known be increased inflammation dysbiosis gut microbiota. In present study, mice were distributed into four groups, subjected for weeks HF, SF,...
Colorectal cancer (CRC), the third most common globally, has shown links to disturbed gut microbiota. While significant efforts have been made establish a microbial signature indicative of CRC using shotgun metagenomic sequencing, challenge lies in validating this with 16S ribosomal RNA (16S) gene sequencing. The primary obstacle is reconciling differing outputs these two methodologies, which often lead divergent statistical models and conclusions. In study, we introduce an algorithm...
Recent studies have revealed the impact of human papillomavirus (HPV) infections on cervicovaginal microbiome; however, few explored utility self-collected specimens (SCS) for microbiome detection, obtained using standardised methods HPV testing. Here, we present a proof-of-concept analysis utilising Oxford Nanopore sequencing 16S rRNA gene in paired samples collected either by patient an Evalyn Brush or physician liquid-based cytology (LBC). We found no significant differences α-diversity...
ABSTRACT Heart failure (HF) is a common condition associated with high rate of hospitalizations and adverse outcomes. HF characterized by impairments the cardiac ventricular filling and/or ejection blood capacity. Sleep fragmentation (SF) involves series short sleep interruptions that lead to fatigue contribute cognitive dementia. Both conditions are known be increased inflammation dysbiosis gut microbiota. In present study, male mice were distributed into four groups, subjected for weeks...