A5079670149- Gut microbiota and health
- Inflammatory Bowel Disease
- Microscopic Colitis
- Probiotics and Fermented Foods
- Gastrointestinal motility and disorders
- Effects of Radiation Exposure
- Pleural and Pulmonary Diseases
- Microbial Metabolites in Food Biotechnology
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Streptococcal Infections and Treatments
- Diabetes Management and Research
- Diabetes and associated disorders
- Colorectal Cancer Screening and Detection
- Liver Disease and Transplantation
- Insect behavior and control techniques
- Radiation Therapy and Dosimetry
- Aortic aneurysm repair treatments
- Cardiac Valve Diseases and Treatments
- Ultrasound in Clinical Applications
- Digestive system and related health
- Liver Disease Diagnosis and Treatment
- Congenital gastrointestinal and neural anomalies
- Gout, Hyperuricemia, Uric Acid
Vall d'Hebron Institut de Recerca
2013-2025
Biomedical Research Institute of Lleida
2023
Universitat de Lleida
2023
Vall d'Hebron Hospital Universitari
1998-2023
Alba Graduate Business School, The American College of Greece
2023
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2010-2023
Instituto de Salud Carlos III
2016-2022
Universitat Autònoma de Barcelona
2004-2022
Centro de Investigación Biomédica en Red
2016-2017
Sorbonne Paris Cité
2014
<h3>Objective</h3> A decade of microbiome studies has linked IBD to an alteration in the gut microbial community genetically predisposed subjects. However, existing profiles dysbiosis adult patients are inconsistent among published studies, and did not allow identification signatures for CD UC. Here, we aimed compare faecal with having UC non-IBD subjects a longitudinal study. <h3>Design</h3> We analysed cohort 2045 samples from four countries (Spain, Belgium, UK Germany), applied 16S rRNA...
The intestinal microbiota consists of over 1000 species, which play key roles in gut physiology and homeostasis. Imbalances the composition this bacterial community can lead to transient dysfunctions chronic disease states. Understanding how manipulate ecosystem is thus essential for treating many disorders. In study, we took advantage recently developed tools deep sequencing phylogenetic clustering examine long-term effects exogenous transplantation combined with without an antibiotic...
Although incrimination of the intestinal microbiota in pathogenesis IBD is widely accepted, few data are available about role specific bacteria. Potentially, Faecalibacterium prausnitzii, bacteria with anti-inflammatory properties, might be deficient ulcerative colitis (UC).To quantify F. prausnitzii faecal UC patients remission and determine its relationship relapse.A cross-sectional study included 116 remission, 29 first-degree relatives 31 healthy controls. A subset eighteen patients,...
Summary Background Inulin and oligofructose promote selective growth of saccharolytic bacteria with low inflammatory potential. Objective To test the effect oligofructose‐enriched inulin in patients active ulcerative colitis. Design Prospective, randomized, placebo controlled pilot trial. Eligible had been previously remission mesalazine as maintenance therapy or no drug, presented a relapse mild to moderate activity. They were treated (3 g/day) randomly allocated receive either (12 g/day,...
INTRODUCTION Severe diarrhea may complicate pelvic radiotherapy and force interruption of treatment. As there is no current clinical or experimental information on the role gut microbiota in this pathogenesis, we conducted a pilot observational study fecal patients receiving radiotherapy. METHODS The involved 10 who underwent 5 wk for abdominal tumors controls. Four samples were collected from each individual: before, during, at end, 2 after Following amplification bacterial 16S rRNA gene...
Abstract The aim of the study was to determine incidence viruses causing aseptic meningitis, meningoencephalitis, and encephalitis in Spain. This a prospective study, collaboration with 17 Spanish hospitals, including 581 cases (CSF from all sera 280): meningitis (340), meningoencephalitis (91), (76), febrile syndrome (7), other neurological disorders (32), 35 without clinical information. CSF were assayed by PCR for enterovirus (EV), herpesvirus (herpes simplex [HSV], varicella‐zoster...
The influence of microbiota in human health is well known. Imbalances microbiome structure have been linked to several diseases. Modulation composition through probiotic therapy an attempt harness the beneficial effects commensal microbiota. Although there wide knowledge responses induced by gut microbiota, microbial factors that mediate these are not Gram-negative bacteria release outer membrane vesicles (OMVs) as a secretion mechanism factors, which important role intercellular...
<h3>Objective</h3> To characterise the influence of diet on abdominal symptoms, anal gas evacuation, intestinal distribution and colonic microbiota in patients complaining flatulence. <h3>Design</h3> Patients flatulence (n=30) healthy subjects (n=20) were instructed to follow their usual for 3 days (basal phase) consume a high-flatulogenic another (challenge phase). <h3>Results</h3> During basal phase, recorded more symptoms than daily questionnaires (5.8±0.3 vs 0.4±0.2 mean discomfort/pain...
Crohn's disease (CD) and ulcerative colitis (UC), the two main forms of inflammatory bowel (IBD), affect several million people worldwide. CD UC are characterized by periods clinical remission relapse. Although IBD patients present chronic alterations gut microbiome, called dysbiosis, little attention has been devoted to relapse-related microbiome. To address this gap, we generated shotgun metagenomic data from stools European cohorts-134 Spanish (followed up for one year) 49 Belgian 6...
Abstract Background Microbiome studies report low gut microbial richness and diversity in ulcerative colitis (UC) patients. We explored whether UC patients who reach long-term clinical, endoscopic, histological remission show a ecosystem that is similar to healthy individuals. Methods collected 184 stool samples from 111 individuals (UC long remission, short flare, control subjects). Microbiota was analyzed by amplicon sequencing (16S ribosomal RNA) quantitative polymerase chain reaction for...
Objective: Nitric oxide has been proposed as a mediator of hyperdynamic circulation in cirrhosis. Endotoxin and cytokines induce the synthesis nitric oxide. The aim this study was to investigate relationship between endotoxemia, cytokines, patients with cirrhosis, correlate these findings clinical, biochemical, hemodynamic parameters. Methods: Clinical, parameters were assessed 66 cirrhosis 15 controls. Levels antidiuretic hormone, plasma renin activity, aldosterone, interferon γ,...
Abstract Background In inflammatory bowel disease, altered microbial states of the gut ecosystem could influence response to treatment. Our objective was investigate possibility predicting vedolizumab using markers microbiome. Methods To this end, we analysed characteristics microbiome shotgun metagenomic sequencing in 21 patients with active ulcerative colitis, eligible for treatment vedolizumab. The protocol included assessment clinical activity and analysis faecal samples prior at weeks...
The response of the fibrinolytic system to inflammatory mediators in empyema and complicated parapneumonic pleural effusions is still uncertain. We prospectively analysed 100 patients with effusion: 25 or effusion, 22 tuberculous 28 malignant effusion transudate effusion. Inflammatory mediators, tumour necrosis factor-α (TNF-α), interleukin-8 (IL-8) polymorphonuclear elastase, were measured serum fluid. Fibrinolytic parameters, plasminogen, tissue-type plasminogen activator (t-PA) urokinase...
Prebiotics have been shown to reduce abdominal symptoms in patients with functional gut disorders, despite that they are fermented by colonic bacteria and may induce gas-related symptoms.To investigate changes the metabolic activity of microbiota induced a recognised prebiotic.Healthy subjects (n = 20) were given prebiotic (2.8 g/day HOST-G904, HOST Therabiomics, Jersey, Channel Islands) for 3 weeks. During 3-day periods immediately before, at beginning end administration put on standard...
Exopolysaccharides (EPS) are extracellular carbohydrate polymers synthesized by a large variety of bacteria. Their physiological functions have been extensively studied, but many their roles not yet elucidated. We sequenced the genomes two isogenic strains Bifidobacterium animalis subsp. lactis that differ in EPS-producing phenotype. The original strain displays nonmucoid appearance, and mutant derived thereof has acquired mucoid sequence analysis revealed nonsynonymous mutation gene...
BackgroundThe remission of Crohn's disease (CD) can be accomplished by faecal microbiota transplantation (FMT). However, this procedure has a low success rate, which could attributed to mis-communication between recipient intestinal mucosa and donor microbiota.MethodsHere we used human explant tissue model an in vivo mouse examine changes upon contact with suspension (FS) obtained from healthy donor. CD patients provided resected inflamed non-inflamed mucosal tissues, whereas control colonic...
Analysis of nondeproteinized samples with an enzymatic method to determine D-lactate indicated interferences. The presence L-lactate dehydrogenase (LD) and in the sample led underestimation content when a blank was processed overestimation it omitted. We proved that this interference is not due lack D-LD stereospecificity. Moreover, assessment L-LD KM for NAD+ allowed us rule out different affinities coenzyme as cause interference. Our results underline importance deproteinizing analysis...
Microbiome sequence data have been used to characterize Crohn's disease (CD) and ulcerative colitis (UC). Based on these data, we previously identified microbiomarkers at the genus level predict CD relapse. However, microbial load was underexplored as a potential biomarker in inflammatory bowel (IBD). Here, sought study use of fungal bacterial loads biomarkers detect both UC We analyzed fecal 294 stool samples obtained from 206 participants using real-time PCR amplification ITS2 region 16S...
In order to know the degree of interleukin-8 (IL-8) production in pleural space and its relationship neutrophil activation, IL-8, elastase (NE), myeloperoxidase (MPO) were assessed blood fluid (PF) 219 patients with effusions. Correlations between PF NE, MPO either absent or weak, except for IL-8 transudates (r = 0.6745, p < 0.001). concentrations cases empyema higher than effusion other causes (p No significant differences inflammatory markers observed parapneumonic tuberculous fluids....
One of the objectives in treatment inflammatory bowel disease (IBD) is improving patient's quality life. However, we do not dispose validated criteria to determine questionnaire's scoring threshold that has be reached order able assert patients' life normalized.To normality punctuation cutoff IBD specific questionnaire IBDQ-36.Cross-sectional study a random sample patients, who have completed questionnaires IBDQ-36 and EuroQol-5D. The was calculated according its equivalence with EuroQol-5D...
Acute intestinal toxicity is a frequent complication that may lead to interruption of treatment in patients undergoing pelvic radiotherapy. Reliable, non-invasive biological markers evidence their severity are not yet available.To test faecal DNA and calprotectin as potential biomarkers caused by radiotherapy.Patients were categorized according the location cancer nonrectal (n = 25) rectal 27). Four stool samples collected at weeks w0, w3, w5 (end radiotherapy) w7. Faecal was determined...