A5017990591- Gut microbiota and health
- Inflammatory Bowel Disease
- Clostridium difficile and Clostridium perfringens research
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Diabetes and associated disorders
- Diverticular Disease and Complications
- Diabetes Management and Research
- Liver Disease and Transplantation
- Liver Disease Diagnosis and Treatment
- Gastrointestinal motility and disorders
- Diet and metabolism studies
- Probiotics and Fermented Foods
- Mycobacterium research and diagnosis
- Respiratory viral infections research
- Transplantation: Methods and Outcomes
- Dermatology and Skin Diseases
- Spaceflight effects on biology
- Blood Coagulation and Thrombosis Mechanisms
- Microbial Metabolites in Food Biotechnology
- Infant Nutrition and Health
- Diet, Metabolism, and Disease
- Colorectal Cancer Surgical Treatments
- Nursing Roles and Practices
- Adenosine and Purinergic Signaling
Population Health Research Institute
2020-2024
McMaster University
2020-2024
Vall d'Hebron Institut de Recerca
2013-2023
Vall d'Hebron Hospital Universitari
2023
UConn Health
2020-2022
Digestive Health Research Institute
2021
<h3>Objective</h3> A decade of microbiome studies has linked IBD to an alteration in the gut microbial community genetically predisposed subjects. However, existing profiles dysbiosis adult patients are inconsistent among published studies, and did not allow identification signatures for CD UC. Here, we aimed compare faecal with having UC non-IBD subjects a longitudinal study. <h3>Design</h3> We analysed cohort 2045 samples from four countries (Spain, Belgium, UK Germany), applied 16S rRNA...
From birth onwards, the human gut microbiota rapidly increases in diversity and reaches an adult-like stage at three years of age. After this age, composition may fluctuate response to external factors such as antibiotics. Previous studies have shown that resilience is not complete months after cessation antibiotic intake. However, little known about short-term effects intake on microbial community. Here we examined load fecal immediately treatment 21 patients, who received broad-spectrum...
Abstract The pathophysiology of irritable bowel syndrome (IBS) remains unclear. Here we investigated the microbiome a large cohort patients to identify specific signatures for IBS subtypes. We examined 113 with and 66 healthy controls. A subset these participants provided two samples one month apart. analyzed total 273 fecal samples, generating more than 20 million 16S rRNA sequences. In IBS, significantly lower microbial diversity was associated relative abundance butyrate-producing...
The microbial community analysis of stools requires optimised and standardised protocols for their collection, homogenisation, disruption nucleic acid extraction. Here we examined whether different layers the stool are equally representative microbiome. We also studied effect water content, which typically increases in diarrhoeic samples, a method on DNA integrity and, therefore, providing an unbiased composition analysis. collected faecal samples from healthy subjects performed by...
Portal hypertension (PH) drives most of the clinical complications in chronic liver diseases. However, its progression nonalcoholic steatohepatitis (NASH) and association with intestinal microbiota (IM) have been scarcely studied. Our aim was to investigate role IM mechanisms leading PH early NASH. The experimental design divided two stages. In stage 1, Sprague‐Dawley rats were fed for 8 weeks a high‐fat, high‐glucose/fructose diet (HFGFD) or control diet/water (CD). Representative selected...
The progression of cirrhosis is associated with alterations in the composition gut microbiome. To assess microbial translocation, we compared serum patients and without ascites characterized ascitic fluid microbiome using 16S rDNA high-throughput sequencing data. A complex specific community was detected but barely detectable healthy controls. presented higher levels lipopolysaccharide binding protein, a marker diversity relative abundance Clostridiales an unknown genus belonging to...
BackgroundFaecal microbiota transplantation (FMT) is a novel potential therapy for inflammatory bowel diseases, but it poorly characterised.MethodsWe evaluated the performance of mouse and rat as pre-clinical model human engraftment. We then characterised effect single stool transfer (HST) on humanised DSS-induced colitis. Colonic faecal microbial communities were analysed using 16S rRNA approach clinical manifestations assessed in longitudinal setting.FindingsThe community rats showed...
Abstract Background and Aims Intestinal microbiota dysbiosis is implicated in Crohn’s disease [CD] may play an important role triggering postoperative recurrence [POR]. We prospectively studied faecal mucosal microbial recolonisation following ileocaecal resection to identify the predictive value of recurrence-related microbiota. Methods Mucosal and/or samples from 121 CD patients undergoing were collected at predefined time points before after surgery. Ileal biopsies 39 healthy controls....
BackgroundThe remission of Crohn's disease (CD) can be accomplished by faecal microbiota transplantation (FMT). However, this procedure has a low success rate, which could attributed to mis-communication between recipient intestinal mucosa and donor microbiota.MethodsHere we used human explant tissue model an in vivo mouse examine changes upon contact with suspension (FS) obtained from healthy donor. CD patients provided resected inflamed non-inflamed mucosal tissues, whereas control colonic...
Emerging evidence implicates microbial proteolytic activity in ulcerative colitis (UC), but whether it also plays a role Crohn's disease (CD) remains unclear. We investigated the effects of colonizing adult and neonatal germ-free C57BL/6 mice with CD microbiota, selected based on high (CD-HPA) or low fecal (CD-LPA), microbiota from healthy controls LPA (HC-LPA) HPA (HC-HPA). then colitogenic mechanisms gnotobiotic C57BL/6, impaired Nucleotide-binding Oligomerization Domain-2 (NOD2)...
<b><i>Background:</i></b> The cross talk between the gut microbiota and immune system, which is essential to maintain homeostasis, takes place at intestinal lymphoid tissue such as mesenteric lymph nodes (MLNs). Here, we investigated presence of bacterial DNA in MLNs control cirrhotic rats its relationship with inflammatory responses. <b><i>Methods:</i></b> MLN microbiome ascites, was induced by carbon tetrachloride...
Probiotics can prevent pathological bacterial translocation by modulating intestinal microbiota and improving the gut barrier. The aim was to evaluate effect of a fermented milk containing Lactobacillus paracasei subsp. CNCM I-1518 on in rats with carbon tetrachloride (CCl4)-induced cirrhosis. Sprague-Dawley treated CCl4 were randomized into probiotic group that received drinking water or only. Laparotomy performed one week after ascites development. We evaluated translocation, microbiota,...
Whether the interaction between gut microbiota and immune response influences evolution of cirrhosis is poorly understood. We aimed to investigate modifications microbiome during progression cirrhosis. Rats were treated with carbon tetrachloride (CCl4) induce then assessed load composition in stool, ileocecal contents (ICCs), mesenteric lymph nodes (MLNs), blood, ascitic fluids (AFs) at 6, 8, 10 weeks or ascites production measured cytokine MLNs blood. The MLN, AF showed a distinct compared...
Imbalances in proteolytic activity have been linked to the development of inflammatory bowel diseases (IBD) and experimental colitis. Proteases intestine play important roles maintaining homeostasis, but exposure mucosal tissues excess can promote pathology through protease-activated receptors (PARs). Previous research implicates microbial proteases IBD, underlying pathways specific interactions between microbes PARs remain unclear. In this study, we investigated role activation external...
Background: Dysbiosis of the intestinal microbiota is implicated in Crohn's disease (CD) and may play an important role triggering postoperative recurrence (POR). We hypothesized that fecal microbial recolonization process after ileocecal resection differs between patients developing remaining remission, further aimed to identify other factors influencing composition. Methods: Fecal samples from 54 CD undergoing were prospectively collected before surgery at month 1, 3 6 surgery. POR -...
nflammatory bowel disease is an immune mediated condition that includes Crohn’s and ulcerative colitis causes chronic inflammation of the gastrointestinal tract. Although exact cause for inflammatory unknown, there consensus a combination genetic, environmental, factors participate in its pathogenesis. To date, all studies have been focused on alterations occur once IBD has developed, however, triggering onset are still unclear. There evident genetic basis which multiple genes involved...
Abstract Objective Type 1 diabetes (T1D) has been associated with alterations of the gut microbiota. Here we investigate cross-talk between immune system and intestinal microbiota in murine T1D. Design To evaluate modulation T1D by microbiota, non-obese diabetic (NOD) mice were cohoused 116C-NOD B-cell transgenic model. further explore influence adaptive NOD models on their fecal studied immunodeficient variants NOD.RAG-2 -/- 116C-NOD.RAG-2 , as well a non-T1D-prone mouse control. The role B...
<b>Introduction & aim:</b> Changes in respiratory bacterial microbiome have been found lung transplantation (LT) and related to chronic allograft dysfunction (CLAD). However, there is less information about changes virome composition LT. The aim of this study was characterize plasma dynamics following LT its possible relationship with CLAD <b>Methods:</b> In retrospective longitudinal study, we analysed the 286 samples from 77 patients at different time-points 20 healthy controls....
Background: Mucosa-associated bacteria are believed to play a more prominent role in the pathogenesis of Crohn's disease (CD) as they closer contact gut immune system. Our aim was study temporal changes microbiota CD patients undergoing ileocecal resection and identify predictive value recurrence-related microbiota. Methods: A total 204 samples from were prospectively collected: biopsies taken resected intestine (histologically inflamed (N=63) non-inflamed ileum (N=56)) neoterminal (N=85)...