A5070135369- Gut microbiota and health
- Celiac Disease Research and Management
- Microscopic Colitis
- Iron Metabolism and Disorders
- Helicobacter pylori-related gastroenterology studies
- Inflammatory Bowel Disease
- Trace Elements in Health
- Hemoglobinopathies and Related Disorders
- Gastrointestinal motility and disorders
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolites in Food Biotechnology
- Diet and metabolism studies
- Pancreatitis Pathology and Treatment
- Autoimmune and Inflammatory Disorders
- Digestive system and related health
- Gastrointestinal disorders and treatments
- IL-33, ST2, and ILC Pathways
- Hereditary Neurological Disorders
- Gene Regulatory Network Analysis
- Agriculture Sustainability and Environmental Impact
- Viral Infectious Diseases and Gene Expression in Insects
- Curcumin's Biomedical Applications
- Eosinophilic Esophagitis
- Probiotics and Fermented Foods
- Tryptophan and brain disorders
McMaster University
2019-2025
Population Health Research Institute
2020-2024
Université de Montréal
2005-2022
Centre Hospitalier de l’Université de Montréal
2009-2019
Jewish General Hospital
2015
McGill University
2015
Centre for Genomic Regulation
2011
Institució Catalana de Recerca i Estudis Avançats
2010
European Bioinformatics Institute
2010
Hôpital Notre-Dame
2005-2009
Metabolism of tryptophan by the gut microbiota into derivatives that activate aryl hydrocarbon receptor (AhR) contributes to intestinal homeostasis. Many chronic inflammatory conditions, including celiac disease involving a loss tolerance dietary gluten, are influenced cues from microbiota. We investigated whether AhR ligand production could influence gluten immunopathology in nonobese diabetic (NOD) mice expressing DQ8, susceptibility gene. NOD/DQ8 mice, exposed or not were subjected three...
Dietary heme can be used by colonic bacteria equipped with heme-uptake systems as a growth factor and thereby impact on the microbial community structure. The of gut microbiota composition may particularly pertinent in chronic inflammation such inflammatory bowel disease (IBD), where strong association dysbiosis has been consistently reported. In this study we investigated influence dietary inferred metagenomic composition, chemically induced colitis colitis-associated adenoma development...
Abstract Microbe-host interactions are generally homeostatic, but when dysfunctional, they can incite food sensitivities and chronic diseases. Celiac disease (CeD) is a sensitivity characterized by breakdown of oral tolerance to gluten proteins in genetically predisposed individuals, although the underlying mechanisms incompletely understood. Here we show that duodenal biopsies from patients with active CeD have increased proteolytic activity against substrates correlates Proteobacteria...
Iron is an important nutrient for both the host and colonizing bacteria. Oral iron supplementation may impact composition of microbiota can be particularly damaging to patients suffering from inflammatory bowel disease (IBD). However, with IBD require treat their anemia.We fed mice diets supplemented ferrous sulfate at different doses (5, 50, 500 mg iron/kg chow) formulations (ferrous sulfate, bisglycinate ferric ethylenediaminetetraacetic acid [FEDTA]), analyzed effects on gut by 16S...
Tryptophan is an essential amino acid transformed by host and gut microbial enzymes into metabolites that regulate mucosal homeostasis through aryl hydrocarbon receptor (AhR) activation. Alteration of tryptophan metabolism has been associated with chronic inflammation; however, whether supplementation affects the metabolite repertoire AhR activation under physiological conditions in humans unknown. We performed a randomized, double blind, placebo-controlled, crossover study 20 healthy...
The authors identified a missense mutation in the <i>FTL</i> gene (474G>A; A96T) 19-year-old man with parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit, and episodic psychosis. This was also present his asymptomatic mother younger brother, who had abnormally low levels of ferritin serum. patient displayed bilateral involvement pallidum.
Abstract Background Gnotobiotic mice colonized with microbiota from patients irritable bowel syndrome (IBS) and comorbid anxiety (IBS+A) display gut dysfunction anxiety‐like behavior compared to healthy volunteers. Using this model, we tested the therapeutic potential of probiotic yeast Saccharomyces boulardii strain CNCM I‐745 ( S . bou ) investigated underlying mechanisms. Methods Germ‐free Swiss Webster were fecal an IBS+A patient or a control (HC). Three weeks later, gavaged daily S....
Abstract Background Celiac disease (CeD) is a T-cell mediated enteropathy driven by gluten in people with risk genes HLA-DQ2 or DQ8. Many patients experience symptoms such as constipation and/or persistent mucosal inflammation despite adherence to gluten-free diet (GFD) and are advised increase dietary fibre. Preliminary results from gluten-immunized NOD-DQ8 mice revealed that supplemented inulin could improve healing. However, whether fibre tolerated metabolized CeD unknown. Aims To...
Abstract Background Celiac disease(CeD) is an autoimmune disease triggered by the ingestion of gluten in genetically predisposed individuals. The only treatment a strict gluten-free diet(GFD), however up to 40% remain symptomatic and have residual inflammation, highlighting need for adjuvant therapies. We showed CeD patients exhibit impaired microbial metabolism tryptophan, resulting reduced activation aryl hydrocarbon receptor(AhR), which plays immunomodulatory roles. Increased AhR ligands...
Abstract Background Impaired microbial indole production and decreased activation of the aryl hydrocarbon receptor (AhR) pathway has been reported in active coeliac disease (CeD). However, whether how this can be targeted by specific therapies is unknown. Aims We tested Saccharomyces boulardii CNCM I-745 (Sbou) influences AhR through stimulation enzymatic activity. Methods Transgenic NOD/DQ8 mice were immunized with partially digested gliadin cholera toxin weekly for 3 weeks followed gluten...
Abstract Background An imbalance in host proteolytic activity (PA) has been reported patients with inflammatory bowel disease (IBD) versus healthy controls (HCs). Fecal PA increases before the diagnosis of ulcerative colitis (UC) which is proinflammatory and a microbial component. High also present Crohn’s (CD), when transferred to germ-free mice, induces inflammation through protease receptor-2 signaling. However, whether high associates symptoms IBD unknown. Aims To investigate have higher...
Abstract BACKGROUND An imbalance in host proteolytic activity (PA) has been reported patients with inflammatory bowel disease (IBD) versus healthy controls (HCs). Fecal PA increases before the diagnosis of ulcerative colitis (UC) which is proinflammatory and a microbial component. High also present Crohn’s (CD), when transferred to germ-free mice, induces inflammation through protease receptor-2 signaling. However, whether high associates symptoms IBD unknown. AIM To investigate have higher...
Hepcidin is a negative regulator of iron absorption produced mainly by the liver in response to changes stores and inflammation, its levels have been shown regulate intestinal basolateral transporter ferroportin1 (Fp1). Hereditary hemochromatosis patients Hfe-deficient mice show inappropriate expression hepcidin but, apparent contradiction, still retain ability alterations metabolism. To further understand molecular relationships among Hfe, hepcidin, Fp1, we investigated Fp1 regulation...
Emerging evidence implicates microbial proteolytic activity in ulcerative colitis (UC), but whether it also plays a role Crohn's disease (CD) remains unclear. We investigated the effects of colonizing adult and neonatal germ-free C57BL/6 mice with CD microbiota, selected based on high (CD-HPA) or low fecal (CD-LPA), microbiota from healthy controls LPA (HC-LPA) HPA (HC-HPA). then colitogenic mechanisms gnotobiotic C57BL/6, impaired Nucleotide-binding Oligomerization Domain-2 (NOD2)...
Here, we propose a framework for the design of synthetic protein networks from modular protein-protein or protein-peptide interactions and provide starter toolkit building blocks. Our proof concept experiments outline general work flow part-based systems engineering. We streamlined iterative BioBrick cloning protocol assembled 25 multidomain proteins each seven standardized DNA fragments. A systematic screen revealed two main factors controlling expression in Escherichia coli: obstruction...