A5108167037- Probiotics and Fermented Foods
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Digestive system and related health
- Infant Nutrition and Health
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Diet and metabolism studies
- Oral microbiology and periodontitis research
- Gastrointestinal motility and disorders
- Escherichia coli research studies
- Microscopic Colitis
- Bacterial Genetics and Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- RNA and protein synthesis mechanisms
- Nosocomial Infections in ICU
- Microbial Metabolic Engineering and Bioproduction
- Legume Nitrogen Fixing Symbiosis
- Drug Transport and Resistance Mechanisms
- Microbial Community Ecology and Physiology
- Viral gastroenteritis research and epidemiology
- Antimicrobial Peptides and Activities
- Plant Pathogenic Bacteria Studies
Loughborough University
2024
Consejo Superior de Investigaciones Científicas
2014-2023
Instituto de Productos Lácteos de Asturias
2014-2023
Instituto de Investigación Sanitaria del Principado de Asturias
2019-2023
Agricultural University of Athens
2022
Gobierno del Principado de Asturias
2020
Ministerio de Ciencia, Innovación y Universidades
2020
Ministry of Science
2020
Universidade de Vigo
2013-2018
University of Minho
2017
Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. The main probiotic bacteria strains belonging to genera Lactobacillus and Bifidobacterium, although other representatives, such as Bacillus or Escherichia coli strains, have also been used. Bifidobacterium two common inhabitants of human intestinal microbiota. Also, some species used food fermentation processes starters, adjunct cultures industry. With exceptions, antibiotic...
ABSTRACT Systemic lupus erythematosus (SLE) is the prototypical systemic autoimmune disease in humans and characterized by presence of hyperactive immune cells aberrant antibody responses to nuclear cytoplasmic antigens, including characteristic anti–double-stranded DNA antibodies. We performed a cross-sectional study order determine if an SLE-associated gut dysbiosis exists patients without active disease. A group 20 SLE remission, for which there was strict inclusion exclusion criteria,...
Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Most of probiotic bacteria currently available market belong to genera Lactobacillus and Bifidobacterium, specific health-promoting activities, such as treatment diarrhea or amelioration gastrointestinal discomfort, have been attributed them. In order be able survive transit transiently colonize our gut, these must counteract deleterious action bile salts, main components bile....
The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation bifidobacteria to gut poorly understood. Analysis 2,214,650-bp genome Bifidobacterium bifidum PRL2010, strain isolated from infant stool, revealed nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling set chromosomal loci...
Assessing the distribution of 16S rRNA gene sequences within a biological sample represents current state-of-the-art for determination human gut microbiota composition. Advances in dissecting microbial biodiversity this ecosystem have very much been dependent on development novel high-throughput DNA sequencing technologies, like Ion Torrent. However, precise representation bacterial community may be affected by protocols used extraction as well PCR primers employed amplification reaction....
The increased availability of genome sequences has advanced the development genomic distance methods to describe bacterial diversity. Results these fast-evolving are highly correlated with those historically standard DNA-DNA hybridization technique. However, genomic-based can be done more rapidly and less expensively prone technical human error. They thus a technically accessible replacement for species delineation. Here, we use several comparison methods, supported by our own proteomic...
Abstract Bifidobacteria are common and frequently dominant members of the gut microbiota many animals, including mammals insects. Carbohydrates considered key carbon sources for microbiota, imposing strong selective pressure on complex microbial consortium gut. Despite its importance, genetic traits that facilitate carbohydrate utilization by still poorly characterized. Here, genome analyses 47 representative Bifidobacterium (sub)species revealed genes predicted to be required degradation...
Abstract Intestinal dysbiosis, characterized by a reduced Firmicutes / Bacteroidetes ratio, has been reported in systemic lupus erythematosus (SLE) patients. In this study, vitro cultures revealed that microbiota isolated from SLE patient stool samples (SLE-M) promoted lymphocyte activation and Th17 differentiation naïve CD4 + lymphocytes to greater extent than healthy control-microbiota. Enrichment of SLE-M with Treg-inducing bacteria showed mixture two Clostridia strains significantly the...
Bifidobacteria represent one of the dominant groups microorganisms colonizing human infant intestine. Commensal bacteria that interact with a eukaryotic host are believed to express adhesive molecules on their cell surface bind specific receptors or soluble macromolecules. Whole-genome transcription profiling Bifidobacterium bifidum PRL2010, strain isolated from stool, revealed small number commonly expressed extracellular proteins, among which were genes specify sortase-dependent pili....
Bifidobacteria represent one of the dominant microbial groups that are present in gut various animals, being particularly prevalent during suckling stage life humans and other mammals. However, overall genome structure this group microorganisms remains largely unexplored. Here, we sequenced genomes 42 representative (sub)species across Bifidobacterium genus used information to explore genetic picture bacterial group. Furthermore, genomic data described here were reconstruct evolutionary...
There is increasing evidence of the key role that gut microbiota plays in inflammatory diseases.To identify differences faecal microbial composition patients with psoriasis compared healthy individuals order to unravel profiling this autoimmune disease.16S rRNA gene sequencing and bioinformatic analyses were performed total DNA extracted from 19 20 same geographic location.Gut displayed a lower diversity different relative abundance certain bacterial taxa individuals.The profile clear...
ABSTRACT Adaptation to and tolerance of bile stress are among the main limiting factors ensure survival bifidobacteria in intestinal environment humans. The effect salts on protein expression patterns Bifidobacterium longum was examined. Protein pattern comparison strains grown with or without extract allowed us identify 34 different proteins whose regulated. majority these were induced after both a minor (0.6 g liter −1 ) major (1.2 exposure bile. These include general response chaperones,...
ABSTRACT Bifidobacteria are one of the main microbial inhabitants human colon. Usually administered in fermented dairy products as beneficial microorganisms, they have to overcome acidic pH found stomach during gastrointestinal transit be able colonize lower parts intestine. The mechanisms underlying acid response and adaptation Bifidobacterium longum biotype NCIMB 8809 its acid-pH-resistant mutant B. 8809dpH were studied. Comparison protein maps, identification by matrix-assisted laser...
Despite the existing evidence on impact of olive oil and red wine intestinal microbiota, effect global Mediterranean Diet (MD) has not been sufficiently studied. We explored association between adherence to a dietary pattern, its components, with faecal microbiota in cohort adults non-declared pathology. This transversal study involved 31 without previous diagnosis cancer, autoimmune or digestive diseases. Based data obtained by means an annual food frequency questionnaire (FFQ), information...
Bifidobacteria are known as anaerobic/microaerophilic and fermentative microorganisms, which commonly inhabit the gastrointestinal tract of various animals insects. Analysis 2,167,301 bp genome Bifidobacterium asteroides PRL2011, a strain isolated from hindgut Apis mellifera var. ligustica, honey bee, revealed its predicted capability for respiratory metabolism. Conservation latter gene clusters in B. strains enforces notion that respiration is common metabolic feature this ancient...
Background: The microbial colonization of the neonatal gut provides a critical stimulus for normal maturation and development. This process early microbiota establishment, known to be affected by several factors, constitutes an important determinant later health. Methods: We studied establishment in preterm full-term infants impact perinatal antibiotics upon this premature babies. To end, 16S rRNA gene sequence-based assessment was performed at phylum level functional inference analyses were...
The microbial populations of the human intestinal tract and their relationship to specific diseases have been extensively studied during last decade. However, characterization bile microbiota as a whole has hampered by difficulties in accessing biological samples lack adequate methodologies assess molecular studies. Although few reports described biliary some hepatobiliary diseases, healthy individuals not described. With this mind, goal present study was generate fundamental knowledge on...