A5067297173- Glycosylation and Glycoproteins Research
- Infant Nutrition and Health
- Probiotics and Fermented Foods
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Breastfeeding Practices and Influences
- Hydrology and Watershed Management Studies
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Helicobacter pylori-related gastroenterology studies
- Digestive system and related health
- Telecommunications and Broadcasting Technologies
- Gastric Cancer Management and Outcomes
- Proteoglycans and glycosaminoglycans research
- Enzyme Catalysis and Immobilization
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Multimedia Communication and Technology
- Flood Risk Assessment and Management
- Ship Hydrodynamics and Maneuverability
- Enzyme Structure and Function
- Soil erosion and sediment transport
Chungnam National University
2016-2025
Kyungpook National University
1999-2023
Electronics and Telecommunications Research Institute
1997-2020
Pukyong National University
2018-2020
Samsung Medical Center
2002-2019
Sungkyunkwan University
2002-2019
Albany College of Pharmacy and Health Sciences
2016-2017
Samsung (South Korea)
2017
Kangwon National University
2012-2013
University of California, Davis
2007-2013
Following birth, the breast-fed infant gastrointestinal tract is rapidly colonized by a microbial consortium often dominated bifidobacteria. Accordingly, complete genome sequence of Bifidobacterium longum subsp. infantis ATCC15697 reflects competitive nutrient-utilization strategy targeting milk-borne molecules which lack nutritive value to neonate. Several chromosomal loci reflect potential adaptation host including 43 kbp cluster encoding catabolic genes, extracellular solute binding...
The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation bifidobacteria to gut poorly understood. Analysis 2,214,650-bp genome Bifidobacterium bifidum PRL2010, strain isolated from infant stool, revealed nutrient-acquisition strategy that targets host-derived glycans, such as those present in mucin. Proteome and transcriptome profiling set chromosomal loci...
Alteration in glycosylation has been observed cancer. However, monitoring changes during breast cancer progression is difficult humans. In this study, we used a well-characterized transplantable tumor mouse model, the mammary virus-polyoma middle T antigen, to observe early glycosylation. We have previously said model look at O-linked with glycan biomarker discovery examined N-linked variations of but time doubling number mice and blood draw points. N-glycans from total serum glycoproteins...
Bifidobacterium longum subsp. infantis (B. infantis) is a common member of the infant intestinal microbiota, and it has been characterized by its foraging capacity for human milk oligosaccharides (HMO). Its genome sequence revealed an overabundance Family 1 solute binding proteins (F1SBPs), part ABC transporters associated with import oligosaccharides. In this study we have used Mammalian Glycan Array to determine specific affinities these proteins. This was correlated protein expression...
Abstract Background and Aims: Epidemiologic studies provide evidence for a link between obesity or diabetes the risk colorectal cancer. However, there is lack of information about relationship metabolic syndrome adenoma. Therefore, we investigated whether factor Methods: We did study consecutive subjects who underwent colonoscopy as screening exam at Center Health Promotion, Samsung Medical Center, from March 2004 to December 2005. According modified ATP III criteria, was diagnosed....
Free oligosaccharides are abundant components of mammalian milk and have primary roles as prebiotic compounds, in immune defense, brain development. A mass spectrometry-based technique is applied to profile from apes (chimpanzee, gorilla, siamang), new world monkeys (golden lion tamarin common marmoset), an old monkey (rhesus). The purpose this study evaluate the patterns primate oligosaccharide composition a phylogenetic perspective assess extent which compositions HMOs derives ancestral...
Most cell membrane proteins are known or predicted to be glycosylated in eukaryotic organisms, where surface glycans essential many biological processes including development and differentiation. Nonetheless, the glycosylation on membranes remains not well characterized because of lack sensitive analytical methods. This study introduces a technique for rapid profiling quantitation N- O-glycans using enrichment nanoflow liquid chromatography/mass spectrometry native structures. Using this new...
Human glycoproteins exhibit enormous heterogeneity at each N-glycosite, but few studies have attempted to globally characterize the site-specific structural features. We developed Integrated GlycoProteome Analyzer (I-GPA) including mapping system for complex N-glycoproteomes, which combines methods tandem mass spectrometry with a database search and algorithmic suite. Using an N-glycopeptide that we constructed, created novel scoring algorithms decoy glycopeptides, where 95 N-glycopeptides...
Significance Deciphering molecular mechanisms at the glycome level in mammalian brain remains a missing piece of puzzle neuroscience due to intrinsic complexity glycosylation and lack analytical tools. Here, we uncovered variation diversity expression human mouse samples according spatial temporal differences. We further constructed comprehensive synthesis map using glycans structurally elucidated by LC-MS/MS found strong evidence on conservation developmental divergence prefrontal cortex...
Abstract Although colorectal cancer (CRC) is considered one of the most preventable cancers, no non-invasive, accurate diagnostic tool to screen CRC exists. We explored potential urine nuclear magnetic resonance (NMR) metabolomics as a for early detection CRC, focusing on advanced adenoma and stage 0 CRC. Urine profiles from patients with neoplasia (CRN; 36 adenomas 56 CRCs at various stages, n = 92) healthy controls (normal, 156) were analyzed by NMR spectroscopy. Healthy CRN groups...
Prioritization of sugar consumption is a common theme in bacterial growth and problem for complete utilization five six carbon sugars derived from lignocellulose. Growth studies show that Lactobacillus brevis simultaneously consumes numerous sources appears to lack normal hierarchical control carbohydrate utilization. Analysis several independent L. isolates indicated co-utilization xylose glucose trait this species. Moreover, carbohydrates can be used as single source are utilized with...
The present paper introduced a computer program, called WISH, which is based on time-domain Rankine panel method. WISH has been developed for practical use to predict the linear and nonlinear ship motion structural loads in waves. adopts three different levels of seakeeping analysis: linear, weakly-nonlinear weak-scatterer approaches. Later, WISH-FLEX consider hydroelasticity effects hull-girder structure. This program can solve springing whipping problems by coupling between hydrodynamic...
Bifidobacterium longum subsp. infantis is a common member of the intestinal microbiota in breast-fed infants and capable metabolizing human milk oligosaccharides (HMO). To investigate bacterial response to different prebiotics, we analyzed both cell wall associated whole proteins B. infantis. Proteins were identified by LC-MS/MS followed comparative proteomics deduce protein localization within cell. Enzymes involved metabolism lactose, glucose, galactooligosaccharides,...
2'-Fucosyllactose (2-FL) is one of the key oligosaccharides in human milk. In present study, salvage guanosine 5'-diphosphate (GDP)-l-fucose biosynthetic pathway from fucose was employed engineered Escherichia coli BL21star(DE3) for efficient production 2-FL. Introduction fkp gene coding fucokinase/GDP-l-fucose pyrophosphorylase (Fkp) Bacteroides fragilis and fucT2 encoding α-1,2-fucosyltransferase Helicobacter pylori allows E. to produce 2-FL fucose, lactose glycerol. To enhance flux...
Biomarkers are needed for noninvasive early detection of gastric cancer (GC). We investigated salivary extracellular RNA (exRNA) biomarkers as potential clinical evaluation tools GC.Unstimulated whole saliva samples were prospectively collected from 294 individuals (163 GC and 131 non-GC patients) who underwent endoscopic at the Samsung Medical Center in Korea. Salivary transcriptomes 63 31 patients profiled, mRNA biomarker candidates verified with reverse transcription quantitative...
Prostate cancer is a leading cause of death among men. Currently available screening test measures prostate‐specific antigen (PSA) to detect prostate cancer. However, this produces false positive values that often lead negative biopsies. Therefore, more reliable diagnostic tool needed. Glycans in serum are particular interest as around half all proteins glycosylated. In study, N‐linked glycans were enzymatically released by PNGase F from epithelial cell lines (pRNS) expressing wild type or...
Marine red macroalgae have emerged to be renewable biomass for the production of chemicals and biofuels, because carbohydrates that form major component can hydrolyzed into fermentable sugars. The main carbohydrate in algae is agarose, it composed D-galactose 3,6-anhydro-L-galactose (AHG), which are alternately bonded by β1-4 α1-3 linkages. In this study, a novel β-galactosidase act on agarooligosaccharides (AOSs) release galactose was discovered marine bacterium (Vibrio sp. strain EJY3);...
Abstract Glycomics, a comprehensive study of glycans expressed in biologic systems, is emerging as simple yet highly sensitive diagnostic tool for disease onset and progression. This aimed to use glycomics investigate glycan markers that would differentiate patients with gastric cancer from those nonatrophic gastritis. Patients duodenal ulcer were also included because they are thought represent biologically different response infection Helicobacter pylori, bacterial can cause either or...
Objectives: Human milk oligosaccharides (HMOs) are known as important factors in neurologic and immunologic development of neonates. Moreover, freeze-drying seems to be a promising storage method improve the processes human banks. However, effects pasteurization on HMOs were not evaluated yet. The purpose this study is analyze compare profiles collected before after freeze-drying.Methods: Totally nine fresh samples from three healthy mothers at first, second, third week delivery. treated...
In clinical settings, biopsies are routinely used to determine cancer type and grade based on tumor cell morphology, as determined via histochemical or immunohistochemical staining. Unfortunately, in a significant number of cases, traditional biopsy results either inconclusive do not provide full subtype differentiation, possibly leading inefficient ineffective treatment. Glycomic profiling the membrane offers an alternate route toward diagnosis. this study, isomer-sensitive nano-LC/MS was...