A5041917681- Gut microbiota and health
- Agriculture and Rural Development Research
- Drug Transport and Resistance Mechanisms
- Pediatric Hepatobiliary Diseases and Treatments
- Diet and metabolism studies
- Animal Nutrition and Physiology
- Heavy metals in environment
- Gallbladder and Bile Duct Disorders
- Clinical Nutrition and Gastroenterology
- Liver Disease Diagnosis and Treatment
- Clostridium difficile and Clostridium perfringens research
- Diet, Metabolism, and Disease
- Digestive system and related health
- Advanced Proteomics Techniques and Applications
- Probiotics and Fermented Foods
- Cholesterol and Lipid Metabolism
- Soil and Land Suitability Analysis
- Crop Yield and Soil Fertility
- Metabolomics and Mass Spectrometry Studies
- Gastrointestinal motility and disorders
- Composting and Vermicomposting Techniques
- Microbial Metabolites in Food Biotechnology
- Bioinformatics and Genomic Networks
- Helicobacter pylori-related gastroenterology studies
- Recycling and Waste Management Techniques
Université Paris-Saclay
2015-2024
Microbiologie de l’alimentation au service de la santé
2014-2024
AgroParisTech
2019-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
1967-2023
Centre d'Immunologie et des Maladies Infectieuses
2021
Fondation Ophtalmologique Adolphe de Rothschild
2021
Pitié-Salpêtrière Hospital
2021
Sorbonne Université
2021
Laboratoire d'Analyses Génétiques pour les Espèces Animales
2015-2021
Rockefeller University
2020
IgA influences bacterial fitness in the intestinal lumen and is only partially compensated for by IgM selective IgA-deficient patients.
In humans, several grams of IgA are secreted every day in the intestinal lumen. While only one isotype exists mice, humans secrete IgA1 and IgA2, whose respective relations with microbiota remain elusive. We compared binding patterns both polyclonal subclasses to commensals glycan arrays determined reactivity profile native human monoclonal antibodies. most dually targeted by IgA2 small intestine, IgA1+IgA2+ IgA1−IgA2+ bacteria coexist colon lumen, where Bacteroidetes is preferentially IgA2....
Abstract Metaproteomics has matured into a powerful tool to assess functional interactions in microbial communities. While many metaproteomic workflows are available, the impact of method choice on results remains unclear. Here, we carry out community-driven, multi-laboratory comparison metaproteomics: critical assessment metaproteome investigation study (CAMPI). Based well-established workflows, evaluate effect sample preparation, mass spectrometry, and bioinformatic analysis using two...
This study was performed with growing chickens (4 to 22 d of age) evaluate the effects feeding a rye-based diet supplemented commercial enzyme preparation containing xylanase and β-glucanase (Quatrazyme HP, Nutri-Tomen, France) on small intestine wall morphology, bile acid composition, nutrient digestibility, bird performance compared unsupplemented rye- or corn-based diets. The decreased (P ≤ 0.05) weight gain, feed intake, efficiency increased water intake diet. Moreover, rye consumption...
The microbial community in the human colon contains bacteria that reduce cholesterol to coprostanol, but species responsible for this conversion are still unknown. We describe here first isolation and characterization of a cholesterol-reducing bacterium intestinal origin. Strain D8 was isolated from 10(-8) dilution fresh stool sample provided by senior male volunteer with high capacity luminal coprostanol. Cholesterol-to-coprostanol strain started on third day, while cells were stationary...
Abstract Background Peptide spectrum matching (PSM) is the standard method in shotgun proteomics data analysis. It relies on availability of an accurate and complete sample proteome that used to make interpretation spectra feasible. Although this procedure has proven be effective many studies, approach limitations when applied complex samples microbial communities, such as those found human intestinal tract. Metagenome studies have indicated microbiome contains over 100 times more genes than...
<h3>Objective</h3> No Crohn's disease (CD) molecular maker has advanced to clinical use, and independent lines of evidence support a central role the gut microbial community in CD. Here we explore feasibility extracting bacterial protein signals relevant CD, by interrogating myriads intestinal proteomes from small number patients healthy controls. <h3>Design</h3> We first developed validated workflow—including extraction communities, two-dimensional difference gel electrophoresis (2D-DIGE),...
Abstract Owing to the growing recognition of gut microbiota as a main partner human health, we are expecting that number indications for fecal transplantation (FMT) will increase. Thus, there is an urgent need standardization entire process transplant production. This study provides complete standardized procedure prepare and store live ready-to-use transplants meet standard requirements good practices applied use in pharmaceutical industry. We show that, if time before transformation would...
The gut microbiota are increasingly considered as a main partner of human health. Metaproteomics enables us to move from the functional potential revealed by metagenomics functions actually operating in microbiome. However, metaproteome deciphering remains challenging. In particular, confident interpretation myriad MS/MS spectra can only be pursued with smart database searches. Here, we compare data sets 48 individual microbiomes using three interrogation strategies dedicated Integrated...
Abstract The promising next-generation probiotic Faecalibacterium prausnitzii is one of the most abundant acetate-consuming, butyrate-producing bacteria in healthy human gut. Yet, little known about how acetate availability affects this bacterium’s gene expression strategies. Here, we investigated effect on temporal changes transcriptome F. duncaniae A2-165 cultures using RNA sequencing. We compared patterns between two growth phases (early stationary vs. late exponential) and levels (low: 3...
The objective of the present study was to evaluate impact a regular consumption yogurt on composition and metabolism human intestinal microbiota. Adult subjects were selected basis daily food records divided into two groups: consumers (at least 200 g consumed per d, n 30); non-consumers (no yogurt, 21). Their faecal microbiota analysed using molecular methods (in situ hybridisation PCR amplification combined with separation by denaturing gel electrophoresis) its metabolic characteristics...
Background: The mechanisms responsible for calorie restriction (CR)-induced improvement in insulin sensitivity (IS) have not been fully elucidated. Greater insight can be achieved through deep biological phenotyping of subjects undergoing CR, and integration big data. Materials Methods: An integrative approach was applied to investigate associations between change IS factors from host, microbiota, lifestyle after a 6-week CR period 27 overweight or obese adults (ClinicalTrials.gov:...
ABSTRACT The Staphylococcus hyicus lip gene was cloned in Lactococcus lactis . Pancreatic insufficiency induced by ligation of the pancreatic duct pigs. In pigs who had undergone ligation, coefficient fat absorption higher after consumption lipase-expressing L. (91.9% ± 3.7%) than that inactive control strain (78.4% 2.4%).