A5052294677- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Pancreatic function and diabetes
- Regulation of Appetite and Obesity
- 3D Printing in Biomedical Research
- Biochemical effects in animals
- Lipid metabolism and biosynthesis
- Liver Disease Diagnosis and Treatment
- Muscle Physiology and Disorders
- Nuclear Structure and Function
- Redox biology and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Advanced Glycation End Products research
- Lipid metabolism and disorders
- Additive Manufacturing and 3D Printing Technologies
- Endoplasmic Reticulum Stress and Disease
- Diet, Metabolism, and Disease
- Biochemical Analysis and Sensing Techniques
- Cellular Mechanics and Interactions
- Mesenchymal stem cell research
- Adipokines, Inflammation, and Metabolic Diseases
Universitat de Barcelona
2018-2023
Institut de Biomedicina de la Universitat de Barcelona
2020-2023
Instituto Maimónides de Investigación Biomédica de Córdoba
2022-2023
University of Córdoba
2022-2023
The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased drastically due to the global obesity pandemic but at present there are no approved therapies. Here, we aimed revert high-fat diet (HFD)-induced and NAFLD in mice by enhancing acid oxidation (FAO). Moreover, searched for potential new lipid biomarkers monitoring steatosis humans. We used adeno-associated virus (AAV) deliver a permanently active mutant form human carnitine palmitoyltransferase 1A (hCPT1AM), key enzyme...
Obesity and its associated metabolic comorbidities are a rising global health social issue, with novel therapeutic approaches urgently needed. Adipose tissue plays key role in the regulation of energy balance adipose tissue-derived mesenchymal stem cells (AT-MSCs) have gained great interest cell therapy. Carnitine palmitoyltransferase 1A (CPT1A) is gatekeeper enzyme for mitochondrial fatty acid oxidation. Here, we aimed to generate adipocytes expressing constitutively active CPT1A form...
Abstract Background Fatty acid metabolism in the hypothalamus has an important role food intake, but its specific AgRP neurons is poorly understood. Here, we examined whether carnitinea palmitoyltransferase 1A (CPT1A), a key enzyme mitochondrial fatty oxidation, affects energy balance. Methods To obtain Cpt1a KO mice and their control littermates, (flox/flox) were crossed with tamoxifen-inducible CreERT2 mice. Food intake body weight analyzed weekly both males females. At 12 weeks of age,...
Acquired muscle diseases such as cancer cachexia are responsible for the poor prognosis of many patients suffering from cancer. In vitro models needed to study underlying mechanisms those pathologies. Extrusion bioprinting is an emerging tool emulate aligned architecture fibers while implementing additive manufacturing techniques in tissue engineering. However, designing bioinks that reconcile rheological needs and biological requirements a challenging matter. Here we formulate biomaterial...
Abstract Orexigenic neurons expressing agouti‐related protein (AgRP) and neuropeptide Y in the arcuate nucleus (ARC) of hypothalamus are activated response to dynamic variations metabolic state, including exercise. We previously observed that carnitine palmitoyltransferase 1a (CPT1A), a rate‐limiting enzyme mitochondrial fatty acid oxidation, is key factor AgRP neurons, modulating whole‐body energy balance fluid homeostasis. However, effect CPT1A aged mice during exercise has not been...
The adipose tissue stores excess energy in the form of neutral lipids within adipocyte lipid droplets (LDs). correct function LDs requires interaction with other organelles, such as endoplasmic reticulum (ER) well LD coat-associated proteins, including Rab18, a mediator intracellular trafficking and ER–LD interaction. Although perturbations inter-organelle contact sites have been linked to several diseases, cancer, no information regarding dysfunctional adipocytes from obese has published...
Background: Obesity is characterized by adipose tissue dysregulation and predisposes individuals to insulin resistance type 2 diabetes. At the molecular level, adipocyte dysfunction has been linked obesity-triggered oxidative stress protein carbonylation, considering carbonylation as a link between metabolic dysfunction. The identification of specific carbonylated proteins in could provide novel biomarkers damage related status (i.e prediabetes). Thus, we aimed at characterizing subcutaneous...
We formulated a highly printable bioink based on GelMA-AlgMA-Fibrin for the automated fabrication of skeletal muscle in vitro models using extrusion bioprinting techniques. modulated photoinitiator concentration to achieve porous materials enhance medium diffusion and promote myoblasts' adhesion, migration, proliferation. To improve mechanical properties biomaterial, we added crosslinked fibrin formulation, which led obtaining 3D cultures that resemble stiffness tissue. The physical together...