A5050320389- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- BRCA gene mutations in cancer
- Nutrition, Genetics, and Disease
- Epigenetics and DNA Methylation
- Nuclear Receptors and Signaling
- Adipose Tissue and Metabolism
- Gene expression and cancer classification
- Genomics and Rare Diseases
- Extracellular vesicles in disease
- RNA Research and Splicing
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Heme Oxygenase-1 and Carbon Monoxide
- Liver Disease Diagnosis and Treatment
- Sex and Gender in Healthcare
- Glutathione Transferases and Polymorphisms
- Lung Cancer Treatments and Mutations
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic Associations and Epidemiology
- Genomics and Phylogenetic Studies
- Endoplasmic Reticulum Stress and Disease
- Skin Protection and Aging
- Ferroptosis and cancer prognosis
Centro de Investigacion Principe Felipe
2016-2025
E-nema (Germany)
2024
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana
2022-2024
Hospital Clínico Universitario de Valencia
2024
Instituto de Investigación Sanitaria La Fe
2024
Institut d'Investigació Sanitària Pere Virgili
2023
Universitat de València
2022-2023
Weatherford College
2020-2023
Instituto de Biomedicina de Valencia
2023
Institute for Integrative Systems Biology
2022
The genetic factors underlying common disease arelargely unknown. Discovery of disease-causing genes willtransform our knowledge the contribution tohuman disease, lead to new screens, and underpinresearch into cures improved lifestyles. sequencing human genome has catalyzed efforts tosearch for by strategy associating sequence variants with measurable phenotypes. In particular, Human Genome Project follow-on tocharacterize variation have resulted in discovery millions single-nucleotide...
Array-CGH is a powerful tool for the detection of chromosomal aberrations. The introduction high-density SNP genotyping technology to genomic profiling, termed SNP-CGH, represents further advance, since simultaneous measurement both signal intensity variations and changes in allelic composition makes it possible detect copy number copy-neutral loss-of-heterozygosity (LOH) events. We demonstrate utility SNP-CGH with two Infinium whole-genome BeadChips, assaying 109,000 317,000 loci,...
Babelomics is a response to the growing necessity of integrating and analyzing different types genomic data in an environment that allows easy functional interpretation results. includes complete suite methods for analysis gene expression include normalization (covering most commercial platforms), pre-processing, differential (case-controls, multiclass, survival or continuous values), predictors, clustering; large-scale genotyping assays (case controls TDTs, population stratification...
Neoadjuvant chemotherapy plus nivolumab has been shown to be effective in resectable non-small-cell lung cancer (NSCLC) the NADIM trial (ClinicalTrials.gov identifier: NCT03081689). The 3-year overall survival (OS) and circulating tumor DNA (ctDNA) analysis have not reported.This was an open-label, multicenter, single-arm, phase II which patients with stage IIIA NSCLC, who were deemed surgically resectable, treated neoadjuvant paclitaxel (200 mg/m2 once a day) carboplatin (area under curve...
Tumors are heterogeneous at the cellular level where ability to maintain tumor growth resides in discrete cell populations. Floating sphere-forming assays broadly used test stem activity tissues, tumors and lines. Spheroids originated from a small population of cells with features able grow suspension culture behaving as tumorigenic mice. We tested eleven common breast cancer lines representing major subtypes mammospheres, measuring viability upon serial non-adherent passage. Only MCF7,...
We have developed a simple and efficient algorithm to identify each member of large collection DNA-linked objects through the use hybridization, applied it manufacture randomly assembled arrays beads in wells. Once has been used determine identity bead, microarray can be wide variety applications, including single nucleotide polymorphism genotyping gene expression profiling. The requires only few labels several sequential hybridizations thousands different DNA sequences with great accuracy....
Mesenchymal stem cells are often transplanted into inflammatory environments where they able to survive and modulate host immune responses through a poorly understood mechanism. In this paper we analyzed the of MSC IL-1β: representative mediator. Microarray analysis treated with IL-1β revealed that cytokine activateds set genes related biological processes such as cell survival, migration, adhesion, chemokine production, induction angiogenesis modulation response. Further more detailed by...
Babelomics has been running for more than one decade offering a user-friendly interface the functional analysis of gene expression and genomic data. Here we present its fifth release, which includes support Next Generation Sequencing data including (RNA-seq), exome or genome resequencing. simplified interface, being now intuitive. Improved visualization options, such as viewer well an interactive network viewer, have implemented. New technical enhancements at both, client server sides, makes...
Heavy binge drinking in adolescence can cause long-term cognitive and behavioral dysfunctions. Recent experimental evidence indicates the participation of immune system activation effects ethanol adolescent brain suggests gender differences. The present study aims to assess plasma cytokine chemokine levels male female adolescents young adults during acute alcohol intoxication correlate these results with toll-like receptor 4 (TLR4) response. potential role TLR4 signaling response was also...
Liver fibrosis constitutes a major health problem worldwide due to its rapidly increasing prevalence and the lack of specific effective treatments. Growing evidence suggests that signalling through cytokine-activated Janus kinase (JAK)-signal transducer activator transcription (STAT) pathways regulates liver regeneration. Rilpivirine (RPV) is widely used anti-HIV drug not reported produce hepatotoxicity. We aimed describe potential hepatoprotective effects RPV in different models chronic...
// Leonor Puchades-Carrasco 1 , Eloisa Jantus-Lewintre 2 Clara Pérez-Rambla 1,2,3 Francisco García-García 4 Rut Lucas Silvia Calabuig Ana Blasco 5 Joaquín Dopazo 4,6,7 Carlos Camps 2,5,8 and Antonio Pineda-Lucena 1,3 Structural Biochemistry Laboratory, Centro de Investigación Príncipe Felipe, Valencia, Spain Molecular Oncology Fundación para la del Hospital General Universitario, 3 Instituto Sanitaria La Fe, Universitario i Politécnico...
Abstract The early transcriptional defense responses and reactive oxygen species (ROS) production in Arabidopsis (Arabidopsis thaliana) cell suspension culture (ACSC), containing functional chloroplasts, were examined at high light (HL). analysis revealed that most of the ROS markers identified among 449 transcripts with significant differential expression specifically up-regulated by singlet (1O2). On contrary, minimal correlation was established superoxide radical or hydrogen peroxide....
Recent results from large-scale genomic projects suggest that allele frequencies, which are highly relevant for medical purposes, differ considerably across different populations. The need a detailed catalog of local variability motivated the whole-exome sequencing 267 unrelated individuals, representative healthy Spanish population. Like in other studies, considerable number rare variants were found (almost one-third described variants). There also differences allelic frequencies...
Thyroid hormones, thyroxine, and triiodothyronine (T3) are crucial for cerebral cortex development acting through regulation of gene expression. To define the transcriptional program under T3 regulation, we have performed RNA-Seq T3-treated untreated primary mouse cerebrocortical cells. The expression 1145 genes or 7.7% expressed was changed upon addition, which 371 responded to in presence cycloheximide indicating direct regulation. results were compared with available transcriptomic...
Abstract Background The incidence of Alzheimer's disease (AD)—the most frequent cause dementia—is expected to increase as life expectancies rise across the globe. While sex-based differences in AD have previously been described, there remain uncertainties regarding any association between sex and disease-associated molecular mechanisms. Studying sex-specific expression profiles regulatory factors such microRNAs (miRNAs) could contribute more accurate diagnosis treatment. Methods A systematic...
Summary Leaf senescence is a complex process, which has dramatic consequences on crop yield. In sunflower, gap between potential and actual yields reveals the economic impact of senescence. Indeed, sunflower plants are incapable maintaining their green leaf area over sustained periods. This study characterizes process in through systems biology approach integrating transcriptomic metabolomic analyses: being grown under both glasshouse field conditions. Our results revealed correspondence...
Recessive dystrophic epidermolysis bullosa (RDEB), Kindler syndrome (KS) and xeroderma pigmentosum complementation group C (XPC) are three cancer‐prone genodermatoses whose causal genetic mutations cannot fully explain, on their own, the array of associated phenotypic manifestations. Recent evidence highlights role stromal microenvironment in pathology these disorders. To investigate, by means comparative gene expression analysis, played dermal fibroblasts pathogenesis RDEB, KS XPC. We...
Abstract Alcohol abuse can induce brain injury and neurodegeneration, recent evidence shows the participation of immune receptors toll-like in neuroinflammation damage. We evaluated role miRNAs as potential modulators associated with alcohol influence TLR4 response. Using mice cerebral cortex next-generation sequencing (NGS), we identified that were differentially expressed chronic alcohol-treated versus untreated WT or TLR4-KO mice. observed a expression miR-183 Cluster (C)...
The knowledge of the genetic variability local population is utmost importance in personalized medicine and has been revealed as a critical factor for discovery new disease variants. Here, we present Collaborative Spanish Variability Server (CSVS), which currently contains more than 2000 genomes exomes unrelated individuals. This database generated collaborative crowdsourcing effort collecting sequencing data produced by genomic projects other purposes. Sequences have grouped ICD10 upper...