A5088303246- Sunflower and Safflower Cultivation
- Plant pathogens and resistance mechanisms
- Genetic diversity and population structure
- Genetic Mapping and Diversity in Plants and Animals
- Powdery Mildew Fungal Diseases
- Genomics and Phylogenetic Studies
- Plant Gene Expression Analysis
- Genetics and Plant Breeding
- Soybean genetics and cultivation
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Light effects on plants
- Plant Genetic and Mutation Studies
- Potato Plant Research
- Plant Taxonomy and Phylogenetics
- Sugarcane Cultivation and Processing
- Herbal Medicine Research Studies
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Genetic and Environmental Crop Studies
- Plant tissue culture and regeneration
- Molecular Biology Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Wheat and Barley Genetics and Pathology
- Plant Pathogens and Resistance
Instituto de Agrobiotecnología del Litoral
2019-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2024
National Agricultural Technology Institute
2015-2024
Instituto de Biotecnología y Biología Molecular
2022
Instituto Nacional de Tecnologia
2019-2022
Agrobiotechnology Institute
2021
National Institute of Industrial Technology
2010-2021
Centro Científico Tecnológico - San Juan
2014
University of Buenos Aires
1993-2010
Fundación Ciencias Exactas y Naturales
2010
Argentina has a long tradition of sunflower breeding, and its germplasm is valuable genetic resource worldwide. However, knowledge the constitution variability levels Argentinean still scarce, rendering global map cultivated diversity incomplete. In this study, 42 microsatellite loci 384 single nucleotide polymorphisms (SNPs) were used to characterize first association mapping population for quantitative trait in sunflower, along with selection allied open-pollinated composite populations...
Development of microsatellite markers for sunflower (Helianthus annuus L.) was performed to estimate their frequency, nature (structure), levels polymorphism, usefulness genotype identification, and calculation genetic relationships between inbred lines representing the species diversity. Isolation from a small-insert genomic library followed by hybridization screening using oligonucleotide probes containing different nucleotide arrays. In this work, 503 unique clones were sequenced 271 PCR...
Abstract Background Considering that sunflower production is expanding to arid regions, tolerance abiotic stresses as drought, low temperatures and salinity arises one of the main constrains nowadays. Differential organ-specific ESTs (expressed sequence tags) were previously generated by a subtractive hybridization method included considerable number putative stress associated sequences. The objective this work analyze concerted gene expression profiles fluorescence microarray assay, in...
Abstract Background Sclerotinia Head Rot (SHR) is one of the most damaging diseases sunflower in Europe, Argentina, and USA, causing average yield reductions 10 to 20 %, but leading total production loss under favorable environmental conditions for pathogen. Association Mapping (AM) a promising choice Quantitative Trait Locus (QTL) mapping, as it detects relationships between phenotypic variation gene polymorphisms existing germplasm without development mapping populations. This article...
Summary Leaf senescence is a complex process, which has dramatic consequences on crop yield. In sunflower, gap between potential and actual yields reveals the economic impact of senescence. Indeed, sunflower plants are incapable maintaining their green leaf area over sustained periods. This study characterizes process in through systems biology approach integrating transcriptomic metabolomic analyses: being grown under both glasshouse field conditions. Our results revealed correspondence...
Phlorisovalerophenone synthase (VPS), a novel aromatic polyketide synthase, was purified to homogeneity from 4.2 mg protein extract obtained hop ( Humulus lupulus L.) cone glandular hairs. The enzyme uses isovaleryl‐CoA or isobutyryl‐CoA and three molecules of malonyl‐CoA form phlorisovalerophenone phlorisobutyrophenone, intermediates in the biosynthesis bitter acids (α‐ β‐acids). VPS is an homodimeric enzyme, with subunits 45 kDa. pI 6.1. K m values 4 µ for isovaleryl‐CoA, 10 33...
Abstract Background Association analysis is a powerful tool to identify gene loci that may contribute phenotypic variation. This includes the estimation of nucleotide diversity, assessment linkage disequilibrium structure (LD) and evaluation selection processes. Trait mapping by allele association requires high-density map, which could be obtained addition Single Nucleotide Polymorphisms (SNPs) short insertion and/or deletions (indels) SSR AFLP genetic maps. diversity randomly selected...
Prosopis alba (Fabaceae) is an important native tree adapted to arid and semiarid regions of north-western Argentina which great value as multipurpose species. Despite its importance, the genomic resources currently available for entire genus are still limited. Here we describe development a leaf transcriptome identification new molecular markers that could support functional genetic studies in natural domesticated populations this genus. Next generation DNA pyrosequencing technology applied...
Snakin-1 is a member of the Solanum tuberosum Snakin/GASA family. We previously demonstrated that involved in plant defense to pathogens as well growth and development, but its mechanism action has not been completely elucidated yet. Here, we showed leaves silenced potato transgenic plants exhibited increased levels reactive oxygen species significantly reduced content ascorbic acid. Furthermore, silencing enhanced salicylic acid accordance with an expression SA-inducible PRs genes....
Restriction site-associated DNA sequencing (RADseq) and its derived protocols, such as double digest RADseq (ddRADseq), offer a flexible highly cost-effective strategy for efficient plant genome sampling. This has become one of the most popular genotyping approaches breeding, conservation, evolution studies in model non-model species. However, universal protocols do not always adapt well to Herein, this study reports development an optimized detailed ddRADseq protocol Eucalyptus dunnii,...
Sunflower Verticillium Wilt and Leaf Mottle (SVW), caused by dahliae Kleb., is a globally prevalent disease affecting sunflower production. In this study, we identified major quantitative trait locus (QTL) on chromosome 10 other genomic regions associated with SVW resistance integrating biparental association mapping in populations from the National Institute of Agricultural Technology. Nine replicated field trials were conducted highly infested V. reservoirs to assess incidence severity....
In recent years, high throughput technologies have led to an increase of datasets from omics disciplines allowing the understanding complex regulatory networks associated with biological processes. Leaf senescence is a mechanism controlled by multiple genetic and environmental variables, which has strong impact on crop yield. Transcription factors (TFs) are key proteins in regulation gene expression, regulating different signaling pathways; their function crucial for triggering and/or...
Abstract Sclerotinia head rot (SHR), caused by the necrotrophic fungus sclerotiorum , is one of most devastating sunflower crop diseases. Despite its worldwide occurrence, genetic determinants plant resistance are still largely unknown. Here, we investigated Sclerotinia-sunflower pathosystem analysing temporal changes in gene expression susceptible and two tolerant inbred lines (IL) inoculated with pathogen under field conditions. Differential analysis showed little overlapping among ILs,...
Sunflower germplasm collections are valuable resources for broadening the genetic base of commercial hybrids and ameliorate risk climate events. Nowadays, most studied worldwide sunflower pre-breeding belong to INTA (Argentina), INRA (France), USDA-UBC (United States America–Canada). In this work, we assess amount distribution diversity (GD) available within between these estimate pattern global diversity. A mixed genotyping strategy was implemented, by combining proprietary...