A5079557997- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- Autophagy in Disease and Therapy
- 3D Printing in Biomedical Research
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Tissue Engineering and Regenerative Medicine
- Cancer Genomics and Diagnostics
- Chemical Reactions and Isotopes
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- Cytokine Signaling Pathways and Interactions
- Occupational Health and Performance
- Wound Healing and Treatments
- RNA Interference and Gene Delivery
- ATP Synthase and ATPases Research
- Hair Growth and Disorders
- Pancreatic function and diabetes
- FOXO transcription factor regulation
- Nonmelanoma Skin Cancer Studies
- Metastasis and carcinoma case studies
Hospital Clínico San Carlos
2023-2024
Universidad Complutense de Madrid
2022-2024
Ministry of Law and Justice
2023-2024
Assistance Publique – Hôpitaux de Paris
2018
Délégation Paris 5
2018
Université Paris Cité
2018
Hôpital Européen
2018
Euskadiko Parke Teknologikoa
2015-2017
Institute of Molecular and Cell Biology
2012-2013
Diputación Foral de Gipuzkoa
2013
Tumors are heterogeneous at the cellular level where ability to maintain tumor growth resides in discrete cell populations. Floating sphere-forming assays broadly used test stem activity tissues, tumors and lines. Spheroids originated from a small population of cells with features able grow suspension culture behaving as tumorigenic mice. We tested eleven common breast cancer lines representing major subtypes mammospheres, measuring viability upon serial non-adherent passage. Only MCF7,...
The rate of inherent resistance to single-agent trastuzumab in HER2-overexpressing metastatic breast carcinomas is impressive at above 70%. Unfortunately, little known regarding the distinctive genetic signatures that could predict refractoriness ab initio. epithelial-to-mesenchymal transition (EMT) molecular features, HER2 expression status and primary responses were explored public Lawrence Berkeley Laboratory (LBL) Breast Cancer Collection. Lentivirus-delivered small hairpin RNAs employed...
// Bruna Corominas-Faja 1,2 , Elisabet Cuyàs Juan Gumuzio 3 Joaquim Bosch-Barrera 4 Olatz Leis 5 Ángel G. Martin and Javier A. Menendez 1 Metabolism & Cancer Group, Translational Research Laboratory, Catalan Institute of Oncology, Girona, Catalonia Spain 2 Girona Biomedical (IDIBGI), Fundación Inbiomed, San Sebastián, Gipuzkoa Medical StemTek Therapeutics; Bilbao, Biscay Correspondence: Menendez, email: Keywords : Acetyl-CoA Carboxylase; Stem Cells;...
Energy metabolism plasticity enables stemness programs during the reprogramming of somatic cells to an induced pluripotent stem cell (iPSC) state. This relationship may introduce a new era in understanding Warburg's theory on metabolic origin cancer at level (CSCs). Here, we used Yamanaka's technology attempt create stable CSC research lines which dissect transcriptional control mTOR—the master switch cellular catabolism and anabolism—in CSC-like states. The rare colonies with iPSC-like...
The ability of somatic cells to reprogram their ATP-generating machinery into a Warburg-like glycolytic metabotype while overexpressing stemness genes facilitates conversion either induced pluripotent stem (iPSCs) or tumor-propagating cells. AMP-activated protein kinase (AMPK) is metabolic master switch that senses and decodes intracellular changes in energy status; thus, we have evaluated the impact AMPK activation regulating generation iPSCs from nonstem origin. indirect direct with...
Targeting tumor-initiating, drug-resistant populations of cancer stem cells (CSC) with phytochemicals is a novel paradigm for prevention and treatment. We herein employed phenotypic drug discovery approach coupled to mechanism-of-action profiling target deconvolution identify phenolic components extra virgin olive oil (EVOO) capable suppressing the functional traits CSC in breast (BC). In vitro screening revealed that secoiridoid decarboxymethyl oleuropein aglycone (DOA) could selectively...
SOX2 is a core pluripotency-associated transcription factor causally related to cancer initiation, aggressiveness, and drug resistance by driving the self-renewal seeding capacity of stem cells (CSC).Here, we tested ability clinically proven inhibitor lysine-specific demethylase 1 (LSD1/KDM1A) iadademstat (ORY-100) target SOX2-driven CSC in breast cancer.Iadademstat blocked CSC-driven mammosphere formation cell lines that are dependent on expression maintain their phenotype.Iadademstat...
Transactivation by c-Rel (nuclear factor κB) was dependent on phosphorylation of several serines in the transactivation domain, indicating that it is a phosphorylation-dependent Ser-rich domain. By Ser → Ala mutational and deletion analysis, we have identified two regions this domain: 1) C-terminal region (amino acids 540–588), which required for basal activity; 2) 422–540 region, responds to external stimuli as tumor necrosis (TNF) α or phorbol myristate acetate plus ionomycin. from 454 473...
The cancer stem cell is defined by its capacity to self-renew, the potential differentiate into all cells of tumor and ability proliferate drive expansion tumor. Thus, targeting these may provide novel anti-cancer treatment strategies. Breast have been isolated according surface marker expression, efflux fluorescent dyes, increased activity aldehyde dehydrogenase or form spheres in non-adherent culture conditions. In order test drugs directed towards modulating self-renewal cells, rapid,...
High rates of inherent primary resistance to the humanized monoclonal antibody trastuzumab (Herceptin) are frequent among HER2 gene-amplified breast carcinomas in both metastatic and adjuvant settings. The clinical efficacy is highly correlated with its ability specifically efficiently target HER2-driven populations cancer stem cells (CSCs). Intriguingly, many possible mechanisms by which escape involve same biomarkers that have been implicated biology CS-like tumor-initiating cells. In...
Resident neural precursor cells (NPCs) have been reported for a number of adult tissues. Understanding their physiological function or, alternatively, activation after tissue damage or in vitro manipulation remains an unsolved issue. Here, we investigated the source human dermal NPCs tissue. By following unbiased, comprehensive approach employing cell-surface marker screening, cell separation, transcriptomic characterization, and vivo fate analyses, found that p75NTR(+) precursors foreskin...
Summary Tumor development and the generation of induced pluripotent stem cells are highly comparable processes with striking similarities. Cellular plasticity is inherent to tumor evolution, rendering that acquire a cell-like phenotype, for which Sox2 activation has proved instrumental plastic acquisition stemness properties in cells. Understanding molecular mechanisms underlying both events might uncover novel approaches anticancer therapeutics constitute model systems understanding...
Background: Hyaluronic acid (HA), glucosamine (Glc), and chondroitin sulfate (CS) are key ingredients commonly incorporated into dietary chondroprotective supplements for the management of osteoarthritis (OA). Despite their widespread use, there is a paucity published data regarding efficacy safety, necessitating rigorous investigation in clinical settings. To address this knowledge gap, we conducted randomized, single-blind pilot study to evaluate effects two commercially available...
Activation of the transcription factor NF-κB is controlled at two levels in resting T cells: an initial activation induced by triggering TcR·CD3 complex and a second phase paracrine- or autocrine-secreted TNFα. The regulated p65 (RelA), whereas one mainly dependent on c-Rel. We describe here mutant clone, D6, derived from parental lymphoblastic line Jurkat that fails to activate upon TNFα stimulation. This clone had no alteration tumor necrosis factor-α (TNFα) signaling pathways nor IκBα,...
The restoration of pluripotency circuits by the reactivation endogenous stemness factors, such as SOX2, may provide a new paradigm in cancer development. tumoral stem cell reprogramming hypothesis, i.e., ability factors to redirect normal and differentiated tumor cells toward less-differentiated stem-like state, adds layers complexity biology, because effects remain dormant until engaged later response (epi)genetic and/or (micro)environmental events. To test this we utilized an vitro model...
The lysine-specific histone demethylase 1A (LSD1) also known as lysine (K)-specific (KDM1A) is a central epigenetic regulator of metabolic reprogramming in obesity-associated diseases, neurological disorders, and cancer. Here, we evaluated the ability oleacein, biophenol secoiridoid naturally present extra virgin olive oil (EVOO), to target LSD1. Molecular docking dynamic simulation approaches revealed that oleacein could binding site LSD1 cofactor flavin adenosine dinucleotide with high...
Complex networks of signaling pathways control the apoptotic response and, therefore, cell survival. However, these converge on a common machinery, which caspase cysteine proteases are key components. Diverse stimuli release holocytochrome c from mitochondria, allowing to bind protease activating factor-1 (Apaf-1), in turn binds caspase-9 both this and forming an Apaf-1/caspase-9 holoenzyme. Cytochromec lacking heme (the apo form) cannot support activation, although reason for has not been...
Previous evidence suggested that NF-kappaB-inducing kinase (NIK) might regulate IL-2 synthesis. However, the molecular mechanism is not understood. In this study, we show NIK involved in CD3 plus CD28 activation of transcription. Splenic T cells from aly/aly mice (that have a defective protein) severe impairment and GM-CSF but TNF secretion response to CD3/CD28. This effect takes place at transcriptional level as overexpression alyNIK inhibits promoter activates responsive element (CD28RE)...
Abstract Lamin A (LMNA)-linked lipodystrophies may be either genetic (associated with LMNA mutations) or acquired the use of human immunodeficiency virus protease inhibitors [PIs]), and in both cases they share clinical features such as anomalous distribution body fat generalized loss adipose tissue, metabolic alterations, early cardiovascular complications. Both LMNA-linked are characterized by accumulation lamin precursor prelamin A. The pathological mechanism which induces lipodystrophy...