A5065737966- Cancer, Lipids, and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Cancer, Hypoxia, and Metabolism
- Venomous Animal Envenomation and Studies
- Fatty Acid Research and Health
- Metabolomics and Mass Spectrometry Studies
- Amino Acid Enzymes and Metabolism
- Venous Thromboembolism Diagnosis and Management
- Epigenetics and DNA Methylation
- Atrial Fibrillation Management and Outcomes
- Healthcare and Venom Research
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Vitamin K Research Studies
- Rabies epidemiology and control
- DNA Repair Mechanisms
- Heparin-Induced Thrombocytopenia and Thrombosis
- Cancer-related Molecular Pathways
- Enzyme Structure and Function
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Lipid Membrane Structure and Behavior
- Cardiac Arrhythmias and Treatments
- Heat shock proteins research
- Medical Imaging Techniques and Applications
Leiden University Medical Center
2015-2024
The University of Sydney
2014-2023
Utrecht University
2019-2023
Centenary Institute
2021
Obesity is associated with increased recurrence and reduced survival of breast cancer. Adipocytes constitute a significant component tissue, yet their role in provisioning metabolic substrates to support cancer progression poorly understood. Here, we show that co-culture cells adipocytes revealed cell-stimulated depletion adipocyte triacylglycerol. Adipocyte-derived free fatty acids were transferred cells, driving acid metabolism via CPT1A electron transport chain complex protein levels,...
Abstract Glutamine is conditionally essential in cancer cells, being utilized as a carbon and nitrogen source for macromolecule production, well anaplerotic reactions fuelling the tricarboxylic acid ( TCA ) cycle. In this study, we demonstrated that glutamine transporter ASCT2 SLC1A5 highly expressed prostate patient samples. Using LNCaP PC ‐3 cell lines, showed chemical or shRNA ‐mediated inhibition of function vitro decreases uptake, cycle progression through E2F transcription factors,...
Prostate cancer cells exhibit altered cellular metabolism but, notably, not the hallmarks of Warburg metabolism. increased de novo synthesis fatty acids (FA); however, little is known about how extracellular FAs, such as those in circulation, may support prostate progression. Here, we show that increasing FA availability intracellular triacylglycerol content cultured patient-derived tumor explants, LNCaP and C4-2B spheroids, a range (LNCaP, C4-2B, 22Rv1, PC-3), epithelial (PNT1)....
Abstract The venom of the Australian snake Pseudonaja textilis comprises powerful prothrombin activators consisting factor X (v-ptFX)- and V-like proteins. While all vertebrate liver-expressed (FX) homologs, including that P. textilis, comprise an activation peptide approximately 45 to 65 residues, v-ptFX is significantly shortened 27 residues. In this study, we demonstrate exchanging human FX for ortholog impedes proteolytic cleavage by intrinsic VIIIa–factor IXa tenase complex....
Direct oral factor (F)Xa inhibitors are widely used as alternatives to conventional vitamin K antagonists in managing venous thromboembolism and nonvalvular atrial fibrillation. Unfortunately, bleeding-related adverse events remain a major concern clinical practice. In case of bleeding or emergency surgery, rapid-onset reversal agents may be required counteract the anticoagulant activity.
4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid (GSAO) when conjugated with a bifunctional chelator 2,2'-(7-(1-carboxy-4-((2,5-dioxopyrrolidin-1-yl)oxy)-4- oxobutyl)-1,4,7-triazonane-1,4-diyl)diacetic (NODAGA) (hereafter referred to as Cell Death Indicator [CDI]), enters dead and dying cells binds 90kDa heat shock proteins (hsp90). This study assesses stability, biodistribution, imaging, radiation dosimetry of [68Ga]- Ga-CDI for positron emission tomography (PET). Preparation...
Background Amino acids such as glutamine are important for tumor cell growth, survival and metabolism. There is renewed interest in metabolism due to the importance of reductive carboxylation cancer. The amino acid transporter ASCT2 (SLC1A5) mediates uptake cancer cells. We have recently reported that expression significantly upregulated melanoma, inhibition decreases uptake, cycle mTORC1 pathway activation [1]. previously shown regulated by androgen receptor prostate [2], this current study...
<p>S1: Pretreatment of PC-3 and C4-2B cells with a FA Mix alters the response to palmitate lipotoxicity serum-starvation. S2: Dose assessment supplementation on cell viability. S3: DGAT1 inhibitor AZD3988 does not affect growth.</p>
<div>Abstract<p>Prostate cancer cells exhibit altered cellular metabolism but, notably, not the hallmarks of Warburg metabolism. Prostate increased <i>de novo</i> synthesis fatty acids (FA); however, little is known about how extracellular FAs, such as those in circulation, may support prostate progression. Here, we show that increasing FA availability intracellular triacylglycerol content cultured patient-derived tumor explants, LNCaP and C4-2B spheroids, a range...
<p>S1: Pretreatment of PC-3 and C4-2B cells with a FA Mix alters the response to palmitate lipotoxicity serum-starvation. S2: Dose assessment supplementation on cell viability. S3: DGAT1 inhibitor AZD3988 does not affect growth.</p>
<div>Abstract<p>Prostate cancer cells exhibit altered cellular metabolism but, notably, not the hallmarks of Warburg metabolism. Prostate increased <i>de novo</i> synthesis fatty acids (FA); however, little is known about how extracellular FAs, such as those in circulation, may support prostate progression. Here, we show that increasing FA availability intracellular triacylglycerol content cultured patient-derived tumor explants, LNCaP and C4-2B spheroids, a range...
The serine protease factor Xa plays an important role in blood coagulation as it, complex with its cofactor Va, proteolytically activates prothrombin to thrombin, the latter being a key enzyme coagulation. To perform this vital function, undergoes numerous posttranslational modifications including vitamin K-dependent γ-carboxylation of glutamic acid (Glu) residues into so-called Gla residues. A correctly γ-carboxylated GLA domain is essential for interaction negatively charged membrane...
OBJECTIVE: The Australian snake venom ptFV (Pseudonaja textilis venom-derived factor V) variant retains cofactor function despite APC (activated protein C)-dependent proteolysis. Here, we aimed to unravel the mechanistic principles by determining role of absent Arg306 cleavage site that is required for inactivation FVa (mammalian Va). APPROACH AND RESULTS: Our findings show in contrast human FVa, APC-catalyzed proteolysis ptFVa at and Lys507 does not abrogate function. Remarkably, structural...