A5042174507- Pain Mechanisms and Treatments
- Pediatric Pain Management Techniques
- Nerve injury and regeneration
- Ion Channels and Receptors
- Exercise and Physiological Responses
- Neurobiology and Insect Physiology Research
- Adenosine and Purinergic Signaling
- Neurofibromatosis and Schwannoma Cases
- Ion channel regulation and function
- Heart Rate Variability and Autonomic Control
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Hereditary Neurological Disorders
- Neuroendocrine regulation and behavior
- Osteoarthritis Treatment and Mechanisms
- Stress Responses and Cortisol
- Muscle Physiology and Disorders
- Cardiac Ischemia and Reperfusion
- Cancer, Stress, Anesthesia, and Immune Response
- Musculoskeletal pain and rehabilitation
- Vagus Nerve Stimulation Research
- Mitochondrial Function and Pathology
- Pain Management and Treatment
- Pain Management and Placebo Effect
- Dermatology and Skin Diseases
- Adipose Tissue and Metabolism
University of Cincinnati Medical Center
2017-2024
Cincinnati Children's Hospital Medical Center
2015-2024
University of Cincinnati
2014-2024
Carl von Ossietzky Universität Oldenburg
2022-2023
Children's Medical Center
2018-2022
University of Pittsburgh
2008-2019
Medical University of Lodz
2017
The Mas-related G protein-coupled receptor D (Mrgprd) is selectively expressed in nonpeptidergic nociceptors that innervate the outer layers of mammalian skin. function Mrgprd nociceptive neurons and physiologically relevant somatosensory stimuli activate Mrgprd-expressing (Mrgprd(+)) are currently unknown. To address these issues, we studied three knock-in mouse lines using an ex vivo preparation to examine role Mrgprd(+) afferents cutaneous somatosensation. In hairy skin, Mrgprd, as marked...
While much is known about the functional properties of cutaneous nociceptors, relatively little comprehensive group III and IV muscle afferents. We have developed a mouse ex vivo forepaw muscle, median ulnar nerve, dorsal root ganglion (DRG), spinal cord recording preparation to examine response properties, neurochemical phenotypes, projections individual found that majority afferents were chemosensitive (52%) while only 34% responded mechanical stimulation fewer (32%) thermal stimuli. The...
The developing peripheral nervous and immune systems are functionally distinct from those of adults. These vulnerable to early-life injury, which influences outcomes related nociception following subsequent injury later in life (i.e., "neonatal nociceptive priming"). underpinnings this phenomenon unclear, although previous work indicates that macrophages trained by inflammation injury. Our findings show both necessary partially sufficient drive neonatal priming, possibly due a long-lasting...
Abstract Studies of human brain indicate that both the ventromedial prefrontal cortex (PFC) and dorsal raphe nucleus (DRN) may be dysfunctional in major depressive illness, making it important to understand functional interactions between these regions. Anatomical studies have shown PFC projects DRN, although synaptic targets this excitatory pathway not yet been identified. Electrophysiological investigations rat DRN report most serotonin neurons are inhibited by electrical stimulation PFC,...
Abstract The paraventricular nucleus of the thalamus (PVT) participates in functional integration limbic cortical and striatal circuitry. In rat, PVT projects to deep layers medial prefrontal cortex (PFC) shell accumbens (NAc). However, synaptic organization afferents within these regions remains undescribed. Furthermore, although dopamine (DA) modulates excitatory glutamate transmission both areas, possible anatomic substrates for specific DA modulation inputs have not yet been...
Damage to peripheral nerves is known contribute chronic pain states, including mechanical and thermal hyperalgesia allodynia. It unknown whether the establishment of these states attributable changes, central modifications, or both. In this study, we used several different approaches assess changes in myelinated (A) unmyelinated (C) cutaneous nociceptors after transection regeneration saphenous nerve. An ex vivo recording preparation was examine response characteristics neurochemical...
Chronic pain conditions are more prevalent in women, but most preclinical studies into mechanisms of generation performed using male animals. Furthermore, whereas group III and IV nociceptive muscle afferents provoke central sensitization effectively than their cutaneous counterparts, less is known about this critical population nociceptors. Here, we compare the physiology individual uninjured males females. We then characterize molecular, physiological, behavioral effects transient ischemia...
Recently, we identified biallelic mutations of SLC25A46 in patients with multiple neuropathies. Functional studies revealed that may play an important role mitochondrial dynamics by mediating fission. However, the cellular basis and pathogenic mechanism SLC25A46-related neuropathies are not fully understood. Thus, generated a Slc25a46 knock-out mouse model. Mice lacking displayed severe ataxia, mainly caused degeneration Purkinje cells. Increased numbers small, unmyelinated degenerated optic...
We have recently found that, following complete Freund's adjuvant (CFA)-induced inflammation, cutaneous polymodal nociceptors (CPM) lacking the transient receptor potential vanilloid 1 (TRPV1) are sensitized to heat stimuli. In order determine possible mechanisms playing a role in this change, we examined gene expression L2/L3 sensory ganglia CFA injection into hairy hind paw skin and that G-protein-coupled purinoreceptor P2Y1 was increased. This is of particular interest, as most CPMs...
Musculoskeletal pain is a significantly common clinical complaint. Although it known that muscles are quite sensitive to alterations in blood flow/oxygenation and number of muscle disorders based problems peripheral perfusion, the mechanisms by which ischemic-like conditions generate myalgia remain unclear. We found, using multidisciplinary experimental approach, ischemia reperfusion injury (I/R) male Swiss Webster mice altered ongoing evoked pain-related behaviors addition activity levels...
Numerous musculoskeletal pain disorders are based in dysfunction of peripheral perfusion and often comorbid with altered cardiovascular responses to muscle contraction/exercise. We have recently found mice that 24 h ischemia induced by a surgical occlusion the brachial artery (BAO) induces increased paw-guarding behaviors, mechanical hypersensitivity, decreased grip strength. These behavioral changes corresponded heat sensitivity as well an increase numbers chemosensitive group III/IV...
It has been well documented that the transient receptor potential melastatin 8 (TRPM8) is involved in environmental cold detection. The role this plays nociception however, somewhat controversial since conflicting reports have shown different neurochemical identities and responsiveness of TRPM8 neurons. In order to functionally characterize cutaneous TRMP8 fibers, we used two ex vivo somatosensory recording preparations neurons innervate hairy skin mice genetically engineered express GFP...
Sensory neurons are chemically and functionally heterogeneous, this heterogeneity has been examined extensively over the last several decades. These studies have employed a variety of different methodologies, including anatomical, electrophysiological, molecular approaches. Recent using next-generation sequencing techniques transcriptome single sensory neurons. Although these reports provided wealth exciting new information on neurons, correlation with functional types is lacking. Here, we...
Pain of unknown etiology is frequent in individuals with the tumor predisposition syndrome neurofibromatosis 1 (NF1), even when tumors are absent. Nerve Schwann cells (SCs) were recently shown to play roles nociceptive processing, and we find that chemogenetic activation SCs sufficient induce afferent behavioral mechanical hypersensitivity wild-type mice. In mouse models, animals showed SCs, but not neurons, lacked Nf1. Importantly, corresponded SC-specific upregulation mRNA encoding glial...