A5030906578- Phagocytosis and Immune Regulation
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Erythrocyte Function and Pathophysiology
- Immune Response and Inflammation
- Neonatal Respiratory Health Research
- Cell Adhesion Molecules Research
- Complement system in diseases
- Immune Cell Function and Interaction
- Asthma and respiratory diseases
- Cell death mechanisms and regulation
- S100 Proteins and Annexins
- Respiratory Support and Mechanisms
- Mast cells and histamine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Platelet Disorders and Treatments
- Immunotherapy and Immune Responses
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Lipid Membrane Structure and Behavior
- Blood disorders and treatments
- Inhalation and Respiratory Drug Delivery
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Food Allergy and Anaphylaxis Research
- Pulmonary Hypertension Research and Treatments
- Immune responses and vaccinations
University of Colorado Denver
2015-2024
University of Colorado Anschutz Medical Campus
1981-2024
National Jewish Health
2015-2024
Pulmonary and Critical Care Associates
2011-2020
SleepMed
2020
Pediatrics and Genetics
2000-2014
University of Colorado Health
1993-2007
New York University
2006
University of Vermont
2004
Millennium Engineering and Integration (United States)
2004
During normal tissue remodeling, macrophages remove unwanted cells, including those that have undergone programmed cell death, or apoptosis. This widespread process extends to the deletion of thymocytes (negative selection), in which cells expressing inappropriate Ag receptors undergo apoptosis, and are phagocytosed by thymic macrophages. Although phagocytosis effete leukocytes has been known since time Metchnikoff, only recently it recognized apoptosis leads surface changes allow...
Apoptosis in vivo is followed almost inevitably by rapid uptake into adjacent phagocytic cells, a critical process tissue remodeling, regulation of the immune response, or resolution inflammation. Phagocytosis apoptotic cells macrophages has been suggested to be quiet that does not lead production inflammatory mediators. Here we show phagocytosis neutrophils (in contrast immunoglobulin G-opsonized cells) actively inhibited interleukin (IL)-1beta, IL-8, IL-10, granulocyte macrophage...
We have studied the leukocyte-dependent mechanism of histamine release (LDHR) from rabbit platelets, a complement-independent which has been implicated in deposition immune complexes acute serum sickness rabbits. It was found by chromatography and passive transfer immunized rabbits that antibody responsible for LDHR IgE type. By electron microscope study reaction, leukocyte involved agglutination platelets their content identified as basophil. Upon addition antigen, basophils sensitized with...
Removal of apoptotic cells is essential for maintenance tissue homeostasis, organogenesis, remodeling, development, and the immune system, protection against neoplasia, resolution inflammation. The mechanisms this removal involve recognition cell surface initiation phagocytic uptake into a variety types. Here we provide evidence that C1q mannose binding lectin (MBL), member collectin family proteins, bind to stimulate ingestion these by ligation on phagocyte multifunctional protein,...
Ingestion of apoptotic cells in vitro by macrophages induces TGF-β1 secretion, resulting an anti-inflammatory effect and suppression proinflammatory mediators. Here, we show vivo that direct instillation enhanced the resolution acute inflammation. This enhancement appeared to require phosphatidylserine (PS) on local induction TGF-β1. Working with thioglycollate-stimulated peritonea or LPS-stimulated lungs, examined cell uptake induction. Viable opsonized human Jurkat T cells, PLB-985...
Ingestion of apoptotic cells in vitro by macrophages induces TGF-β1 secretion, resulting an anti-inflammatory effect and suppression proinflammatory mediators. Here, we show vivo that direct instillation enhanced the resolution acute inflammation. This enhancement appeared to require phosphatidylserine (PS) on local induction TGF-β1. Working with thioglycollate-stimulated peritonea or LPS-stimulated lungs, examined cell uptake induction. Viable opsonized human Jurkat T cells, PLB-985...
The strongest susceptibility genes for the development of systemic lupus erythematosus (SLE) in humans are null mutants classical pathway complement proteins. There is a hierarchy disease and severity according to position missing protein activation pathway, with severest associated C1q deficiency. Here we demonstrate, using novel vivo models apoptotic cell clearance during sterile peritonitis, similar hierarchical role proteins cells by macrophages. Our results constitute first...
Successful repair after tissue injury and inflammation requires resolution of the inflammatory response removal extracellular matrix breakdown products. We have examined whether cell-surface adhesion molecule hyaluronan receptor CD44 plays a role in resolving lung inflammation. CD44-deficient mice succumb to unremitting following noninfectious injury, characterized by impaired clearance apoptotic neutrophils, persistent accumulation fragments at site activation transforming growth factor–β 1...
Removal of apoptotic cells during tissue remodeling or resolution inflammation is critical to the restoration normal structure and function. During apoptosis, early surface changes occur, which trigger recognition removal by macrophages other phagocytes. Loss phospholipid asymmetry results in exposure phosphatidylserine (PS), one markers recognized macrophages. However, a number receptors have been reported mediate macrophage cells, not all bind phosphatidylserine. We therefore examined role...
We investigated the capacity of bacterial endotoxin (lipopolysaccharide, LPS) to modify oxidative metabolic response membrane stimulation human neutrophils. Neutrophils were pretreated for 60 min with LPS, 10 ng/ml, then stimulated by exposure fixed immune complexes, chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP), or phorbol myristate acetate. Release superoxide anion (O-2) was up 7-times greater in cells preincubated depending upon stimulus used. Consumption oxygen and...
One of the key features associated with programmed cell death in many tissues is phagocytosis apoptotic bodies by macrophages. Removal cells occurs before their lysis, indicating that these cells, during development apoptosis, express specific surface changes recognized We have compared mechanisms which four different macrophage populations recognize cells. Murine macrophages elicited into peritoneal cavity either two phlogistic agents were able to phagocytose This was inhibited...
The neutrophil has been implicated as an important mediator of vascular injury, especially after endotoxemia. This study examines neutrophil-mediated injury to human microvascular endothelial cells in vitro. We found that neutrophils stimulated by formyl-methionyl-leucyl-phenylalanine (FMLP), the complement fragment C5a, or lipopolysaccharide (LPS) (1-1,000 ng/ml) alone produced minimal over a 4-h assay. In contrast, incubated with presence low concentrations LPS (1-10 could then be FMLP C5a...
Efficient phagocytosis of apoptotic cells is important for normal tissue development, homeostasis, and the resolution inflammation. Although many receptors have been implicated in clearance cells, roles these engulfment process not well defined. We developed a novel system to distinguish between involved tethering versus those inducing their uptake. Our results suggest that regardless engaged on phagocyte, ingestion does occur absence phosphatidylserine (PS). Further, recognition PS was...