A5036713844- Retinal Development and Disorders
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Visual perception and processing mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Circadian rhythm and melatonin
- Antimicrobial Resistance in Staphylococcus
- Tuberculosis Research and Epidemiology
- Traumatic Brain Injury and Neurovascular Disturbances
- Immune Response and Inflammation
- Retinal Diseases and Treatments
- Intensive Care Unit Cognitive Disorders
- Mycobacterium research and diagnosis
- Berberine and alkaloids research
- Diagnosis and treatment of tuberculosis
- Nicotinic Acetylcholine Receptors Study
- Zebrafish Biomedical Research Applications
- Anesthesia and Neurotoxicity Research
- Streptococcal Infections and Treatments
- Color Science and Applications
- Cell Adhesion Molecules Research
- RNA regulation and disease
- Antibiotic Resistance in Bacteria
University of Washington
2020-2024
Seattle University
2022
The University of Texas Health Science Center at Houston
2005-2020
Wake Forest University
2020
Forest Institute
2020
Charlottesville Medical Research
2020
John Wiley & Sons (United States)
2020
Mitchell Institute
2013
Texas Children's Hospital
2008
Baylor College of Medicine
2008
To describe the wide-field ganglion cells, they were injected intracellularly with Neurobiotin using an in vitro preparation of macaque retina and labeled streptavidin-Cy3. The retinas then antibodies to choline acetyltransferase other markers indicate depth dendrites within inner plexiform layer (IPL) analyzed by confocal microscopy. There eight different subtypes narrowly unistratified cells that ramified each 5 strata, S1-5, including narrow thorny, large sparse, moderate, dense, radiate,...
ABSTRACT In this study, we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from canine commensal pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion of progenitor epidermal keratinocytes (CPEK). Invasion was examined by gentamicin protection assay fluorescence microscopy. An Δ spsD ΔspsL mutant strain ED99 had a dramatically reduced capacity to invade CPEK monolayers, while no difference level observed with single mutants....
Abstract Midget retinal ganglion cells (RGCs) are the most common RGC type in primate retina. Their responses have been proposed to mediate both color and spatial vision, yet specific links between midget visual perception unclear. Previous research on dual roles of RGCs has focused those comparing long (L) vs. middle (M) wavelength sensitive cones. However, there is evidence for several other rare subtypes receiving S-cone input, but their role vision uncertain. Here, we confirm existence...
Abstract Traumatic brain injury (TBI) causes a profound inflammatory response within the central nervous system and peripheral immune system, which contributes to secondary further morbidity mortality. Preclinical investigations have demonstrated that treatments downregulate microglia activation polarize them toward reparative/anti-inflammatory phenotype improved outcomes in preclinical models. However, no therapy date has translated into proven benefits human patients. Regulatory T cells...
Microglial cells are the primary resident immune in retina. In healthy adults, they ramified; that is, have extensive processes move continually. adult retinas, microglia maintain normal structure and function of neurons other glial cells, but mechanism underlying this process is not well-understood. mouse hippocampus, engulf small pieces axons presynaptic terminals via a called trogocytosis. Here we report macaque retina also at sites synapses. We analyzed volume serial, ultrathin sections...
Abstract A Neurobiotin‐injected OFF parasol cell from midperipheral macaque retina was studied by reconstruction of serial ultrathin sections and compared with ON cells previously. In most respects, the synaptic inputs to two subtypes were similar. Only a few amacrine processes that provided input labeled ganglion dendrites made or received within series, none these interactions bipolar other presynaptic cell. These findings suggest direct inhibitory originates areas retina. known receive...
Abstract Parasol cells are one of the major types primate retinal ganglion cells. The goal this study was to describe synaptic inputs that shape light responses ON type parasol cells, which excited by increments in intensity. A connectome from central macaque retina generated serial blockface scanning electron microscopy. Six neighboring were reconstructed, and their analyzed. On average, they received 21% input bipolar excitatory local circuit neurons receiving cones. majority amacrine...
Abstract There are more than 30 distinct types of mammalian retinal ganglion cells, each sensitive to different features the visual environment. In rabbit retina, they can be grouped into four classes according their morphology and stratification dendrites in inner plexiform layer (IPL). The goal this study was describe synaptic inputs one type Class IV cell, third member sparsely branched cells (SB3). One cell partially reconstructed a connectome developed using automated transmission...
A labeled ON parasol ganglion cell from a macaque retina was analyzed in serial, ultrathin sections. It received 13% of its input diffuse bipolar cells. These directed large proportion their output to amacrine cells but relatively small via feedback synapses. In these respects, they were similar the DB3 that make synapses onto OFF Bipolar axons contacted stratum 4 inner plexiform layer always made dendrite, and therefore, number could be estimated reliably by light microscopy future....
ABSTRACT We examined the antibody responses of pediatric patients infected with community-associated Staphylococcus aureus isolates. The data show that Panton-Valentine leukocidin (PVL)-positive strains developed a dominant immunoglobulin G anti-PVL response correlates markers inflammation.
Considerable progress has been made in studying the receptive fields of most common primate retinal ganglion cell (RGC) types, such as parasol RGCs. Much less is known about rarer RGC types and circuitry that gives rise to noncanonical field structures. The goal this study was analyze synaptic inputs smooth monostratified RGCs determine origins their complex spatial fields, which contain isolated regions high sensitivity called "hotspots." Interestingly, co-stratify with well-studied are...
Purpose: To describe serotonergic retinopetal axons in monkeys. Methods: Whole macaque and baboon retinas, fixed 4% paraformaldehyde, were labeled with antisera raised against serotonin (5-HT). Results: Several large-diameter 5–HT-immunoreactive (IR) emerged from the optic disk. Most ran to peripheral retina, where they branched extensively. terminated ganglion cell layer, but a few 5-HT-IR distal inner plexiform or within nuclear layer. Some extensively near fovea, dense plexus of was also...
Abstract In primates, broad thorny retinal ganglion cells are highly sensitive to small, moving stimuli. They have tortuous, fine dendrites with many short, spine‐like branches that occupy three contiguous strata in the middle of inner plexiform layer. The neural circuits generate their responses stimuli not well‐understood, and was goal this study. A connectome from central macaque retina generated by serial block‐face scanning electron microscopy, a cell reconstructed, its synaptic inputs...
Ganglion cells are the projection neurons of retina. Intrinsically photosensitive retinal ganglion (ipRGCs) express photopigment melanopsin and also receive input from rods cones via bipolar amacrine cells. In primates, multiple types ipRGCs have been identified. The with somas in cell layer studied extensively, but less is known about those inner nuclear layer, "displaced" To investigate their synaptic inputs, three sets horizontal, ultrathin sections through central macaque retina were...
Abstract Midget retinal ganglion cells (RGCs) are the most common RGC type in primate retina. Their responses mediate both color and spatial vision, yet specific links between midget visual perception unclear. Previous research on dual roles of RGCs has focused those comparing long (L) vs. middle (M) wavelength sensitive cones. However, there is evidence for several other rare subtypes receiving S-cone input, but their role vision uncertain. Here, we confirm existence single center OFF...