A5018841163- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Biochemical and Structural Characterization
- Cell Adhesion Molecules Research
- Antimicrobial Peptides and Activities
- Neonatal and Maternal Infections
- Infective Endocarditis Diagnosis and Management
- Orthopedic Infections and Treatments
- Force Microscopy Techniques and Applications
- Pneumonia and Respiratory Infections
- Peptidase Inhibition and Analysis
- Bacterial Identification and Susceptibility Testing
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Oral microbiology and periodontitis research
- Wound Healing and Treatments
- Toxin Mechanisms and Immunotoxins
- Glycosylation and Glycoproteins Research
- Bone Tissue Engineering Materials
- Protease and Inhibitor Mechanisms
- Bacterial Infections and Vaccines
- S100 Proteins and Annexins
University of Pavia
2016-2025
University of Birmingham
2023
Laboratoire de Biochimie
2020
Politecnico di Milano
2006
Trinity College Dublin
2004
Universität Ulm
2004
University of Alabama
1989-1992
Rigshospitalet
1992
University of Sassari
1987-1988
Bayer (Germany)
1985
Summary Staphylococcus aureus strains isolated from patients with septic arthritis or osteomyelitis possess a collagen receptor present in two forms, which contains either three copies of 187‐amino‐acid repeat motif. Collagen receptor‐positive adhered to both substrata and cartilage time‐dependent process. receptor‐specific antibodies blocked bacterial adherence, as did preincubation the substrate recombinant form protein. Furthermore, polystyrene beads coated bound attached cartilage. Taken...
Twenty-one genes encoding surface proteins belonging to the LPXTG family have been identified by in silico analysis of six Staphylococcus aureus genome sequences. Eleven encode previously described proteins, while 10 not yet characterized. Of these, eight contain cell-wall sorting signal responsible for covalently anchoring peptidoglycan. The remaining two, SasF and SasD, harbour a single residue variation fourth position motif (LPXAG). Western blotting lysostaphin-solubilized S....
The SasG surface protein of Staphylococcus aureus has been shown to promote the formation biofilm. comprises an N-terminal A domain and repeated B domains. Here we demonstrate that is involved in accumulation phase biofilm, a process requires physiological concentration Zn(2+). domains, but not domain, are required. Purified recombinant can form dimers vitro Zn(2+)-dependent fashion. Furthermore, bind cells have domains anchored their block biofilm formation. full-length exposed on cell...
Summary Staphylococcus aureus is a leading cause of infective endocarditis (IE). Platelet activation promoted by S. resulting in aggregation and thrombus formation an important step the pathogenesis IE. Here, we report that fibrinogen/fibronectin‐binding proteins FnBPA FnBPB are major platelet‐activating factors on surface from exponential phase growth. Truncated derivatives FnBPA, presenting either fibrinogen‐binding A domain or fibronectin‐binding BCD region, each platelet when expressed...
Staphylococci are important causes of nosocomial and medical-device-related infections. Their virulence is attributed to the elaboration biofilms that protect organisms from immune system clearance increased resistance phagocytosis antibiotics. Photodynamic treatment (PDT) has been proposed as an alternative approach for inactivation bacteria in biofilms. In this study, we have investigated effect photodynamic action toluidine blue O (TBO) on viability structure Staphylococcus epidermidis a...
Attachment of bacteria to the host tissue is considered a first step in development many infections. Previous studies have shown that fibronectin, protein mediate substrate adhesion eukaryotic cells, also binds some pathogenic and mediates adherence these prokaryotes. In present communication, we report on isolation characterization fibronectin receptor from Staphylococcus aureus strain Newman. A 210-kDa binding was isolated bacterial lysate by affinity chromatography followed gel...
Although several risk factors for stroke have been identified, one-third remain unexplained. Here we show that infection with Streptococcus mutans expressing collagen-binding protein (CBP) is a potential factor haemorrhagic stroke. Infection serotype k S. mutans, but not standard strain, aggravates cerebral haemorrhage in mice. Serotype accumulates the damaged, contralateral hemisphere, indicating an interaction of bacteria injured blood vessels. The most important high-virulence expression...
Staphylococcus epidermidis is a ubiquitous human skin commensal that has emerged as major cause of foreign-body infections. Eleven genes encoding putative cell-wall-anchored proteins were identified by computer analysis the publicly available S. unfinished genomic sequence. Four encode previously described (Aap, Bhp, SdrF and SdrG), while remaining seven have not been characterized. Analysis primary sequences S taphylococcus e pidermidis s urface (Ses) indicates they structural organization...
Staphylococcus aureus is responsible for a wide range of infections, including soft tissue infections and potentially fatal bacteremias. The primary niche S. in humans the nares, nasal carriage documented risk factor staphylococcal infection. Previous studies with rodent models colonization have implicated capsule teichoic acid as surface factors that promote colonization. In this study, mouse model was utilized to demonstrate mutants lack clumping A, collagen binding protein, fibronectin...
Significance Hospital-acquired infections often involve surface-associated microbial communities called biofilms that show increased resistance to antibiotics. A molecular understanding of the fundamental interactions driving adhesion and biofilm formation by pathogens is a key step toward development novel antimicrobial therapies. Here we demonstrate SasG surface protein from Staphylococcus aureus displays remarkable zinc-dependent mechanical properties are critical for its adhesive...
ABSTRACT The second immunoglobulin-binding protein (Sbi) of Staphylococcus aureus has two N-terminal domains that bind the Fc region IgG in a fashion similar to A and can complement C3 promote its futile consumption fluid phase. It been proposed Sbi helps bacteria avoid innate immune defenses. By comparing mutant defective with mutants A, clumping factor iron-regulated surface determinant H, capsular polysaccharide, it was shown is indeed an evasion promotes bacterial survival whole human...
Staphylococcus epidermidis can express three different cell-surface-associated proteins, designated SdrF, SdrG and SdrH, that contain serine-aspartate dipeptide repeats. Proteins SdrF are similar in sequence structural organization to the Sdr proteins of aureus comprise unique 625- 548-residue A regions at their N termini, respectively, followed by 110–119-residue B-repeat SD-repeat regions. The C termini LPXTG motifs hydrophobic amino acid segments characteristic surface covalently anchored...
The ability of Staphylococcus aureus to adhere components the extracellular matrix is an important mechanism for colonization host tissues during infection. We have previously shown that S. binds elastin, a major component matrix. integral membrane protein, elastin-binding protein (EbpS), soluble elastin peptides and tropoelastin via its surface-exposed N-terminal domain. In this study, we demonstrate some strains strongly immobilized human interaction independent EbpS but instead mediated...