A5018489774- Bacillus and Francisella bacterial research
- Inflammatory mediators and NSAID effects
- Eicosanoids and Hypertension Pharmacology
- Infective Endocarditis Diagnosis and Management
- Cancer, Lipids, and Metabolism
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Ocular Infections and Treatments
- Oral microbiology and periodontitis research
- Inhalation and Respiratory Drug Delivery
- Microbial Inactivation Methods
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- RNA and protein synthesis mechanisms
- Estrogen and related hormone effects
- Peroxisome Proliferator-Activated Receptors
- Synthesis and biological activity
- Nanoplatforms for cancer theranostics
- Protein purification and stability
- Yersinia bacterium, plague, ectoparasites research
- Fatty Acid Research and Health
- Biochemical and Structural Characterization
- Free Radicals and Antioxidants
Fudan University
2025
Jiujiang University
2024
Vanderbilt University Medical Center
2023
Texas A&M Health Science Center
2010-2023
The University of Texas Health Science Center at Houston
2000-2023
Affiliated Eye Hospital of Wenzhou Medical College
2016-2023
Wenzhou Medical University
2016-2023
National Clinical Research
2023
National Clinical Research Center for Digestive Diseases
2021
The University of Texas Health Science Center at San Antonio
2021
Streptococcus gallolyticus subsp. (Sg) has long been known to have a strong association with colorectal cancer (CRC). This knowledge important clinical implications, and yet little is about the role of Sg in development CRC. Here we demonstrate that promotes human colon cell proliferation manner depends on context, bacterial growth phase direct contact between bacteria cells. In addition, observed increased level β-catenin, c-Myc PCNA cells following incubation Sg. Knockdown or inhibition...
Staphylococcus epidermidis is a ubiquitous human skin commensal that has emerged as major cause of foreign-body infections. Eleven genes encoding putative cell-wall-anchored proteins were identified by computer analysis the publicly available S. unfinished genomic sequence. Four encode previously described (Aap, Bhp, SdrF and SdrG), while remaining seven have not been characterized. Analysis primary sequences S taphylococcus e pidermidis s urface (Ses) indicates they structural organization...
Streptococcus gallolyticus subspecies ( Sgg ) has a strong clinical association with colorectal cancer (CRC) and actively promotes the development of colon tumors. However, molecular determinants involved in pathogenicity gut are unknown. Bacterial type VII secretion systems (T7SS) mediate pathogen interactions their host important for virulence pathogenic mycobacteria Staphylococcus aureus . Through genome analysis, we identified locus strain TX20005 that encodes putative system (designated...
ABSTRACT Rifaximin is a poorly absorbed semisynthetic antibiotic derivative of rifampin licensed for use in the treatment traveler's diarrhea. reduces symptoms enteric infection, often without pathogen eradication and with limited effects on intestinal flora. Epithelial cells (HEp-2 [laryngeal], HCT-8 [ileocecal], A549 [lung], HeLa [cervical]) were pretreated rifaximin (or control antibiotics) prior to addition enteroaggregative Escherichia coli (EAEC). EAEC adherence was significantly...
Streptococcus gallolyticus subsp. (Sg) has long been reported to display a strong association with colorectal cancer (CRC). It was recently demonstrated actively promote the development of CRC, underscoring importance Sg in both clinical correlation and functional relevance CRC. Here we investigated several isolates their interactions human colon cells mouse models. Some strains were able stimulate host cell proliferation (proliferation-promoting Sg, PP-Sg) whereas others not...
Abstract Streptococcus gallolyticus subspecies ( Sgg ) is known to be strongly associated with colorectal cancer (CRC). Recent functional studies further demonstrated that actively stimulates CRC cell proliferation and promotes the development of colon tumors. However, factors important for pro-proliferative pro-tumor activities remain unclear. Here, we identified a chromosomal locus in strain TX20005. Deletion this significantly reduced adherence cells abrogated ability stimulate...
ABSTRACT Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli . The antigen was not detectable E. strains, however. Here, show by reverse transcriptase-PCR the 16 genes this region are transcribed OG1RF. Gene disruption orfde4 , encoding putative glycosyl transferase, and orfde6 dTDP-rhamnose biosynthesis gene, generated two mutants. mutants showed delayed killing higher 50% lethal dose mouse...
ABSTRACT Our previous work identified a cosmid clone containing 43-kb insert from Enterococcus faecalis OG1RF that produced nonprotein antigen in Escherichia coli . In the present work, we studied this detail. Periodate treatment of lysates confirmed was carbohydrate nature. Analysis DNA sequences and transposon insertion mutants suggested contained multicistronic gene cluster. Database comparison showed cluster genes similar to involved biosynthesis dTDP-rhamnose, glycosyltransferases, ABC...
We recently reported that knockdown of delta-5-desaturase (a key enzyme converts dihomo-γ-linolenic acid, DGLA, to the downstream ω-6 arachidonic acid) promotes formation an anti-cancer byproduct 8-hydroxyoctanoic acid from cyclooxygenase (COX)-catalyzed DGLA peroxidation. can exert its growth inhibitory effect on cancer cells (e.g. colon and pancreatic cancer) by serving as a histone deacetylase inhibitor. Since inhibitors have been well-known suppress cell migration invasion, we thus...
Cyclooxygenase-2 (COX-2), the inducible COX form, is a bi-functional membrane-bound enzyme that typically metabolizes arachidonic acid (downstream ω-6 fatty acid) to form 2-series of prostaglandins known be involved in cancer development. Overexpression COX-2 has been found majority breast carcinomas, and also associated with increased severity development metastasis. Our lab recently demonstrated can metabolize dihomo-γ-linolenic (DGLA, precursor produce an anti-cancer byproduct,...
The current model for Bacillus anthracis dissemination in vivo focuses on macrophages as carriers. However, recent evidence suggested that other host cells may also play a role the process. Here, we tested possibility of B. being internalized by human fibroblast cell line, HT1080 and an epithelial Caco-2. A combination gentamicin protection assays, scanning transmission electron microscopy (EM) fluorescence was used. results demonstrated first time both spores vegetative Sterne strain 7702...
Dissemination of Bacillus anthracis spores from the lung is a critical early event in establishment inhalational anthrax. We recently reported that B. could adhere to and be internalized by cultured intestinal epithelial fibroblast cells. Here, using gentamicin protection assays and/or electron microscopy, we found Sterne strain 7702 were able subsequently polarized A549 cells primary human small airway showed for first time survive translocate across an cell barrier apical side basolateral...
Spores of Bacillus anthracis, the causative agent anthrax, are known to persist in host lungs for prolonged periods time, however underlying mechanism is poorly understood. In this study, we demonstrated that BclA, a major surface protein B. anthracis spores, mediated direct binding complement factor H (CFH) spores. The bound CFH retained its regulatory cofactor activity resulting C3 degradation and inhibition downstream activation. By comparing results from wild type C57BL/6 mice deficient...
We have previously demonstrated that DGLA treatment along with Delta-5-Desaturase (D5D) siRNA in various types of cancer cells enhances the formation 8-HOA from COX-2-catalyzed peroxidation, which turn inhibits cell growth and migration. However, delivery naked remains a formidable challenge due to its "off-target" effect. In this study, we employed RNA nanotechnology for specific D5D-siRNA xenograft colon tumors using 3WJ nanoparticles. When targeting module, i.e., EpCAM aptamer, was...
Dihomo-γ-linolenic acid (DGLA) and its downstream fatty arachidonic (AA) are both nutritionally important ω–6 polyunsaturated acids (ω–6s). Evidence shows that, via COX-mediated peroxidation, DGLA metabolites (1-series prostaglandins) associated with anti-tumor activity, while AA (2-series could be tightly implicated in various cancer diseases. However, it still remains a mystery why possess contrasting bioactivities. Our previous studies showed that go through an exclusive C-8 oxygenation...
Abstract Background There is growing evidence indicating that the microbial communities dwell on human ocular surface are crucially important for health and disease. Little known about interspecies interactions, functional profiles, strain heterogeneity across individuals in healthy microbiomes. Methods To comprehensively characterize heterogeneity, cooccurrence network, taxonomic composition profile of microbiome, we performed shotgun metagenomics sequencing mucosal membrane swabs 17...
Inhalational anthrax is initiated by pulmonary exposure to Bacillus anthracis spores. Spore entry into lung epithelial cells observed both in vitro and vivo evidence suggests it important for bacterial dissemination virulence. However the specific host receptor spore factor that mediate process were unknown. Here, we report integrin α2β1 a major entry. This supported results from blocking antibodies, siRNA knock-down, colocalization, comparison of do or not express α2. BclA, surface protein,...
Inhalational anthrax is initiated by the entry of Bacillus anthracis spores into lung. A critical early event in establishment an infection dissemination from Using vitro cell culture assays, we previously demonstrated that B. are capable entering epithelial cells lung and crossing a barrier without apparent disruption integrity, suggesting novel portal for to disseminate However, vivo evidence spore uptake has been lacking. Here, using mouse model, present taken up soon after delivered...