A5048251367- Phagocytosis and Immune Regulation
- Erythrocyte Function and Pathophysiology
- Immune cells in cancer
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Blood groups and transfusion
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Blood properties and coagulation
- Diabetes and associated disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Complement system in diseases
- Cell death mechanisms and regulation
- Blood disorders and treatments
- T-cell and B-cell Immunology
- Platelet Disorders and Treatments
- Neonatal Health and Biochemistry
- Nerve injury and regeneration
- Pancreatitis Pathology and Treatment
- Blood transfusion and management
- Protein Tyrosine Phosphatases
- Adenosine and Purinergic Signaling
- S100 Proteins and Annexins
- Epigenetics and DNA Methylation
Umeå University
2012-2024
Laboratoire d’immunologie intégrative du cancer
2013
Washington University in St. Louis
2000-2005
Rockefeller University
2005
University of New Mexico
2002
University of Geneva
2002
University of Washington
2001
The immune system recognizes invaders as foreign because they express determinants that are absent on host cells or lack “markers of self” normally present. Here we show CD47 (integrin-associated protein) functions a marker self murine red blood cells. Red lacked were rapidly cleared from the bloodstream by splenic pulp macrophages. normal prevented this elimination binding to inhibitory receptor signal regulatory protein alpha (SIRPα). Thus, macrophages may use number nonspecific activating...
High macrophage infiltration has been correlated to improved survival in colorectal cancer (CRC). Tumor associated macrophages (TAMs) play complex roles tumorigenesis since they are believed hold both tumor preventing (M1 macrophages) and promoting (M2 activities. Here we have applied an immunohistochemical approach determine the degree of infiltrating with a M1 or M2 phenotype clinical specimens CRC relation prognosis, general but also subgroups defined by microsatellite instability (MSI)...
In autoimmune hemolytic anemia (AIHA), circulating red blood cells (RBCs) opsonized with autoantibody are recognized by macrophage Fcgamma and complement receptors. This triggers phagocytosis elimination of RBCs from the circulation splenic macrophages. We recently found that CD47 on unopsonized binds signal regulatory protein alpha (SIRPalpha), generating a negative prevents RBCs. show here clearance is also regulated CD47-SIRPalpha. The inhibition generated CD47-SIRPalpha interaction...
Interactions between cells and their surroundings are important for proper function homeostasis in a multicellular organism. These interactions can either be established the molecules extracellular milieu, but also involve cells. In all these situations, proteins plasma membranes critically involved to relay information obtained from exterior of cell. The cell surface glycoprotein CD47 (integrin-associated protein (IAP)) was first identified as an regulator integrin function, later shown...
Abstract Src homology 2 domain-containing protein tyrosine phosphatase (SHP) substrate-1 (SHPS-1) is a transmembrane that expressed predominantly in macrophages. Its extracellular region interacts with the ligand CD47 on surface of adjacent cells, and its cytoplasmic binds phosphatases SHP-1 SHP-2. Phagocytosis IgG- or complement-opsonized RBCs by peritoneal macrophages derived from mice express mutant SHPS-1 lacks most was markedly enhanced compared apparent wild-type This effect not...
Integrin-associated protein (CD47) is a broadly expressed that costimulates T cells, facilitates leukocyte migration, and inhibits macrophage scavenger function. To determine the role of CD47 in regulating alloresponses, CD47(+/+) or CD47(-/-) cells were infused into irradiated nonconditioned major histocompatibility complex disparate recipients. Graft-versus-host disease lethality was markedly reduced with cells. Donor failed to engraft immunodeficient allogeneic marrow unable reconstitute...
Abstract Background Traumatic injury to axons produces breakdown of and myelin at the site lesion then further distal this where Wallerian degeneration develops. The rapid removal degenerated by phagocytosis is advantageous for repair since molecules in impede regeneration severed axons. Thus, revealing mechanisms that regulate macrophages microglia important. We hypothesize regulates its own simultaneous activation down-regulation microglial macrophage responses. Activation follows binding...
Cancer-associated fibroblasts expressing fibroblast activation protein (FAP) have been implicated in the invasive behavior of colorectal cancer. In this study, we use FAP expression as a marker and analyze effect activated on cancer migration invasion experimental cell studies. We also investigated pattern cancer-associated during transformation from benign to malignant tumors. immunohistochemical analyses, was expressed all samples examined, whereas normal colon, hyperplastic polyps, or...
A characteristic subset of microglia expressing CD11c appears in response to brain damage. However, the functional role CD11c+ microglia, as well mechanism its induction, are poorly understood. Here we report that genetic ablation signal regulatory protein α (SIRPα), a membrane protein, induced emergence white matter. Mice lacking CD47, physiological ligand SIRPα, and microglia-specific SIRPα-knockout mice exhibited same phenotype, suggesting an interaction between microglial SIRPα CD47 on...
Macrophages recognize CD47 as a marker of "self" and phagocytose CD47(null) hematopoietic cells. Using chimera models, here, we show that the phagocytic activity macrophages against cells is conferred by expression on nonhematopoietic cells, this "education" process cell-independent. in chimeras where express whereas those lacking are tolerant to However, latter retain RBCs, demonstrating split macrophage tolerance The findings highlight potential importance regulation function, suggest...
Macrophages are cells with many important functions in both innate and adaptive immune responses have been shown to play a complex role tumor progression since they harbour preventing (M1 macrophages) promoting (M2 activities. In human cancers, infiltrating macrophages associated poor patient prognosis, therefore suggested be mainly of an M2 phenotype. However, we others previously that increased macrophage density colorectal cancer (CRC) instead is correlated improved prognosis. It...
Macrophages play a complex role in tumor progression since they can exert both tumor-preventing (M1 macrophages) and tumor-promoting (M2 activities. In colorectal carcinoma (CRC), at odds to many other cancers, macrophage infiltration has been correlated with an improved patient survival. recent study, we have evaluated the distribution of M1 M2 subtypes CRC their impact on prognosis.
The development of axons and dendrites is controlled by small GTP-binding proteins the Rho family, but upstream signaling mechanisms responsible for such regulation remain unclear. We have now investigated role transmembrane protein cluster differentiation 47 (CD47) in this process with hippocampal neurons. CD47-deficient neurons manifested markedly impaired axons, whereas overexpression CD47 promoted development. Interaction SH2 domain-containing tyrosine phosphatase substrate-1 (SHPS-1)...
Fas (CD95) mediates apoptosis of many cell types, but the susceptibility cells to killing by ligand and anti-Fas antibodies is highly variable. Jurkat T lacking CD47 (integrin-associated protein) are relatively resistant Fas-mediated death efficiently killed or IgM (CH11) upon expression CD47. Lack impairs events downstream activation including caspase activation, poly-(ADP-ribose) polymerase cleavage, cytochrome c release from mitochondria, loss mitochondrial membrane potential, DNA...
Red blood cell (RBC) clearance is known to occur primarily in the spleen, and presumed be executed by red pulp macrophages. Erythrophagocytosis spleen takes place as part of homeostatic turnover RBCs remove old RBCs. It can strongly promoted immunoglobulin G (IgG) opsonization RBCs, a condition that consequence autoantibody or alloantibody formation. The purpose our study was investigate which phagocytes are involved IgG-mediated RBC human spleen. We developed highly specific vitro assay...
Abstract CD47 is a ubiquitously expressed cell surface glycoprotein that associates with integrins and regulates chemotaxis, migration, activation of leukocytes. also ligand for signal regulatory protein α, receptor on monocytes, macrophages, granulocytes, dendritic (DC) subsets activation, adhesion, migration. Although the function in macrophages granulocytes has been studied detail, little known about role DC biology vivo. In this study we demonstrate CD47−/− mice exhibit selective...