A5001078030- Pain Mechanisms and Treatments
- Marine and coastal plant biology
- Polymer Surface Interaction Studies
- Temporomandibular Joint Disorders
- Marine Biology and Ecology Research
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Chemical Synthesis and Analysis
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Transcranial Magnetic Stimulation Studies
- RNA modifications and cancer
- Advanced Polymer Synthesis and Characterization
- Medical and Biological Sciences
- Oral microbiology and periodontitis research
- Knee injuries and reconstruction techniques
- Drug Solubulity and Delivery Systems
- Dental Implant Techniques and Outcomes
- Endodontics and Root Canal Treatments
- DNA Repair Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Dental materials and restorations
- Laser Applications in Dentistry and Medicine
- RNA Interference and Gene Delivery
Murata (Japan)
2024-2025
Kanazawa University
2020-2024
RIKEN Center for Integrative Medical Sciences
2019-2024
Nagoya University
2024
Yokohama City University
2021-2023
Mie University
2014-2022
Niigata University of Pharmacy and Medical and Life Sciences
2015-2021
University of Fukui
2018-2020
Kyushu University
2010-2019
Osaka University
2006-2019
Long-term treatments to ameliorate peripheral neuropathic pain that includes mechanical allodynia are limited. While glial activation and altered nociceptive transmission within the spinal cord associated with pathogenesis of allodynia, changes in cortical circuits also accompany nerve injury may represent additional therapeutic targets. Dendritic spine plasticity S1 cortex appears days following injury; however, underlying cellular mechanisms this whether it has a causal relationship remain...
Abstract Microglia survey and directly contact neurons in both healthy damaged brain, but the mechanisms functional consequences of these contacts are not yet fully elucidated. Combining two-photon imaging patch clamping, we have developed an acute experimental model for studying role microglia CNS excitotoxicity induced by neuronal hyperactivity. Our allows us to simultaneously examine effects repetitive supramaximal stimulation on axonal morphology, membrane potential, microglial...
Defective mitochondrial dynamics through aberrant interactions between mitochondria and actin cytoskeleton is increasingly recognized as a key determinant of cardiac fragility after myocardial infarction (MI). Dynamin-related protein 1 (Drp1), fission-accelerating factor, activated locally at the fission site with actin. Here, we report that actin-binding filamin A acted guanine nucleotide exchange factor for Drp1 mediated fission-associated senescence in mice MI. In peri-infarct regions...
Autophagy is primarily activated by cellular stress, such as starvation or mitochondrial damage. However, stress-independent autophagy unclear mechanisms in several cell types, thymic epithelial cells (TECs). Here we report that the protein, C15ORF48, a critical inducer of autophagy. Mechanistically, C15ORF48 reduces membrane potential and lowers intracellular ATP levels, thereby activating AMP-activated protein kinase its downstream Unc-51-like 1. Interestingly, C15ORF48-dependent induction...
Abstract RAD51 assembly on single-stranded (ss)DNAs is a crucial step in the homology-dependent repair of DNA damage for genomic stability. The formation filament promoted by various RAD51-interacting proteins including paralogues. However, mechanisms underlying differential control RAD51-filament dynamics these factors remain largely unknown. Here, we report role human paralogue, SWSAP1, as novel regulator assembly. Swsap1 -deficient cells show defects damage-induced during both mitosis and...
A characteristic subset of microglia expressing CD11c appears in response to brain damage. However, the functional role CD11c+ microglia, as well mechanism its induction, are poorly understood. Here we report that genetic ablation signal regulatory protein α (SIRPα), a membrane protein, induced emergence white matter. Mice lacking CD47, physiological ligand SIRPα, and microglia-specific SIRPα-knockout mice exhibited same phenotype, suggesting an interaction between microglial SIRPα CD47 on...
Activation of astrocytes has a profound effect on brain plasticity and is critical for the pathophysiology several neurological disorders including neuropathic pain. Here, we show that metabotropic glutamate receptor 5 (mGluR5), which reemerges in restricted time frame, essential these functions. Although mGluR5 absent healthy adult astrocytes, it transiently somatosensory cortex (S1). During limited spatiotemporal astrocytic drives Ca2+ signals; upregulates multiple synaptogenic molecules...
The aim of this study was to prepare, using taste-masked granules, tablets which can rapidly disintegrate in saliva (rapidly disintegrating tablet), drugs with bitter taste (pirenzepine HCl or oxybutynin HCl). granules were prepared aminoalkyl methacrylate copolymers (Eudragit E-100) by the extrusion method. None dissolved from (% dissolved, < 5%) even at 480 min pH 6.8 dissolution test. However, medium 1.2 Rapidly and a mixture excipients consisting crystalline cellulose (Avicel PH-102)...
Examination of Listeria monocytogenes prevalence among ready-to-eat foods in Japan revealed frequent (5.7 to 12.1%) contamination minced tuna and fish roe products, the isolates had same virulence levels as clinical terms invasion efficiency infectivity cell cultures a murine infection model, respectively. Premature stop codons inlA were infrequent (1 out 39 isolates). Cell numbers L. salmon increased rapidly under inappropriate storage temperatures (from most probable number [MPN] 10(0)...
Early-life stress may cause several neuropsychological disorders in adulthood. Such be induced as a result of instability neuronal circuits and/or synaptic formation. However, the mechanisms underlying such have not yet been clearly understood. We previously reported that mushroom spine somatosensory cortex (SSC) is unstable early-life stressed mice only juvenile stage but also In this study, we measured number and motility microglial processes to understand mechanism further. found...
Na,K-ATPase is a ubiquitous molecule contributing to the asymmetrical distribution of Na+ and K+ ions across plasma membrane maintenance potential, prerequisite neuronal activity. comprises three subunits (α, β, FXYD). The α subunit has four isoforms in mice, with them (α1, α2, α3) expressed brain. However, functional biological significances different brain remain be fully elucidated. Recent studies have revealed association Atp1a3, gene encoding α3 subunit, neurological disorders. To map...
Medullary thymic epithelial cells (mTECs) are critical for self-tolerance induction in T via promiscuous expression of tissue-specific antigens (TSAs), which controlled by the transcriptional regulator, AIRE. Whereas AIRE-expressing (Aire + ) mTECs undergo constant turnover adult thymus, mechanisms underlying differentiation postnatal remain to be discovered. Integrative analysis single-cell assays transposase-accessible chromatin (scATAC-seq) and RNA sequencing (scRNA-seq) suggested...
The mechanism by which acute pain or itch information at the periphery is processed in primary somatosensory cortex (S1) remains unclear. To elucidate this, we used a viral-mediated targeted-recombination-in-active population system to target S1 neuronal ensembles that are active during sensations. We induced expression of excitatory inhibitory designer receptors exclusively activated drugs pain- itch-related neurons. identified populations mice regulate sensory components and hind paw...
In this study, we demonstrate a novel and efficient fabrication methodology for nonclose-packed, two-dimensional (2D) colloidal crystals exhibiting square lattice structures. our recent work, detailed the formation of 2D via electrostatic adsorption three-dimensional (3D) charged onto oppositely substrates. These 3D possessed face-centered cubic (FCC) structure with their (111) planes aligned parallel to substrate, facilitating triangular arrangements upon adsorption. This work presents...
To decrease the sensation of roughness when a tablet, which is rapidly disintegrated by saliva (rapidly disintegrating tablet), orally taken, we prepared tablets using microcrystalline cellulose (Avicel PH-M series), new type pharmaceutical excipient that spherical and has very small particle size (particle size, 7-32 microm), instead conventional (PH-102) used in formulation containing acetaminophen or ascorbic acid as model drugs for tableting study. Tablets (200 mg) cellulose, PH-M-06,...
Abstract Peripheral nerve injury causes maladaptive plasticity in the central nervous system and induces chronic pain. In addition to injured limb, abnormal pain sensation can appear limb contralateral injury, called mirror image Because synaptic remodeling primary somatosensory cortex (S1) has critical roles induction of pain, cortical reorganization S1 ipsilateral may also accompany To elucidate this, we conducted vivo 2-photon calcium imaging neuron astrocyte activity after a peripheral...
Poly(1-(2-methacryloyloxy)ethyl-3-butylimidazolium bis(trifluoromethanesulfonyl)imide) (PMIS) and poly(n-hexyl methacrylate) (PHMA) brushes were prepared on initiator-immobilized silicon wafers by surface-initiated atom transfer radical polymerization. The macroscopic frictional properties of the determined using a ball-on-flat type tribotester under reciprocating motion in dry nitrogen atmosphere, water, methanol, 1-ethyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide (EMImTFSI)....