A5049118958- Enzyme Structure and Function
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Helminth infection and control
- Microbial Metabolic Engineering and Bioproduction
- Amino Acid Enzymes and Metabolism
- Biochemical and Molecular Research
- Probiotics and Fermented Foods
- Peptidase Inhibition and Analysis
- Coccidia and coccidiosis research
- Enzyme Production and Characterization
- Ubiquitin and proteasome pathways
- Protein Structure and Dynamics
- Animal Nutrition and Physiology
- Polyamine Metabolism and Applications
- Identification and Quantification in Food
- Medical Imaging Techniques and Applications
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Advanced MRI Techniques and Applications
- Biofuel production and bioconversion
- Cancer, Hypoxia, and Metabolism
- Primate Behavior and Ecology
- Pancreatic function and diabetes
- Research on Leishmaniasis Studies
The University of Tokyo
2016-2025
University of Miyazaki
2013-2025
Tokyo Women's Medical University
2020-2022
Tokyo Women's Medical University Yachiyo Medical Center
2020
Kobe Asahi Hospital
2020
Biotechnology Research Center
2016
Tokyo Women's Medical University Hospital
2014
Nihon University
2011-2012
Osaka University
2008-2012
Toranomon Hospital
2009
Enzymes initiating the biosynthesis of cellular building blocks are frequently inhibited by end-product respective pathway. Here we present an approach to rapidly generate sets enzymes overriding this control. It is based on in vivo detection desired single cells using a genetically encoded sensor. The sensor transmits intracellular product concentrations into graded optical output, thus enabling ultrahigh-throughput screens FACS. We randomly mutagenized plasmid-encoded ArgB Corynebacterium...
Lysine residues can be post-translationally modified by various acyl modifications in bacteria and eukarya. Here, we showed that two major modifications, acetylation succinylation, were changed response to the carbon source Gram-positive model bacterium Bacillus subtilis. Acetylation was more common when cells grown on glucose, glycerol, or pyruvate, whereas succinylation upregulated citrate, reflecting metabolic states preferentially produce acetyl-CoA succinyl-CoA, respectively. To...
To determine a profile of gene expression in retinas murine model oxygen-induced retinopathy (OIR).OIR was induced C57BL/6N mice by exposing postnatal day (P)7 pups to 75% oxygen for 5 days and then returning them room air at P12. Gene microarrays containing more than 47,000 transcripts were used study the changes isolated immediately (P12) 12 hours (P12.5) after exposure hyperoxia. The P12 raised under normoxic conditions served as control subjects. Quantitative RT-PCR multiplex ELISA...
Parasite diversity has important implications in several research fields including ecology, evolutionary biology and epidemiology. Wide-ranging analysis been restricted because of the difficult, highly specialised time-consuming processes involved parasite identification. In this study, we assessed wild rats using 18S rDNA-based metagenomics. rDNA PCR products were sequenced an Illumina MiSeq sequencer sequences QIIME software successfully classified them into groups. The comparison results...
Abstract The bacterium Corynebacterium glutamicum is utilized during industrial fermentation to produce amino acids such as l ‐glutamate. During ‐glutamate fermentation, C . changes the flux of central carbon metabolism favor production, but molecular mechanisms that explain these remain largely unknown. Here, we found profiles two major lysine acyl modifications were significantly altered upon glutamate overproduction in ; acetylation decreased, whereas succinylation increased. A label‐free...
In recent years, genome mining in cyanobacteria has revealed abundant gene clusters related to natural product biosynthesis. However, only a few terpene synthases (TSs) have been identified from this bacterial phylum. Pfam profiles, such as PF03936 and PF19086, which are frequently used for TS retrieval, built plant, bacterial, fungal TSs. Herein, we constructed new hidden Markov model (HMM) specific TSs on the basis of 110 experimentally validated years. Using model, pair diterpene...
The diterpene cyclase CotB2 catalyzes the cyclization of geranylgeranyl diphosphate (GGPP) to tricyclic cyclooctat-9-en-7-ol, which is characterized by a 5-8-5-fused ring skeleton. We have previously proposed cascade involving unique carbon–carbon bond rearrangement combined with multiple hydride shifts, all occurring at single active site. Here, we report first high-resolution X-ray crystal structure bound substrate analog thiodiphosphate (GGSPP). In GGSPP-bound form, GGSPP folds into...
Abstract Plants respond to herbivory by perceiving herbivore danger signal(s) (HDS(s)), including “elicitors”, that are present in herbivores’ oral secretions (OS) and act induce defense responses. However, little is known about HDS-specific molecules intracellular signaling. Here we explored soybean receptor-like kinases (RLKs) as candidates might mediate HDS-associated RLKs’ (HAKs’) actions leaves response OS extracted from larvae of a generalist herbivore, Spodoptera litura ....
Susceptibility to Plasmodium chabaudi depends on the relative dominance of Th1/Th2 responses in host mice. A Th2-dominant response during early phase infection susceptible A/J mice causes a fatal disease course due severe malaria. Schistosoma mansoni is potent inducer not only parasite antigens, but also other antigens concurrently existing animals. In spite S. infection, these escape death from malaria and showed accompanied enhanced production IFN-γ antigens. Treatment with anti-IFN-γ mAb...
1. Changes in membrane capacitance evoked by the rapid photolysis of a caged Ca2+ compound, DM‐nitrophen or nitrophenyl‐EGTA, were investigated undifferentiated PC12 cells. They interpreted as representing exocytosis and endocytosis. 2. The jumps two components exocytosis. Slow was selectively with small increases intracellular concentration between 5 10 microM, while fast preceded slow one at [Ca2+]i greater than microM. 3. release rates depended steeply on [Ca2+]i. A half‐maximal rate...
Aspartate kinase (AK) is the first and committed enzyme of biosynthetic pathway producing aspartate family amino acids, lysine, threonine, methionine. AK from Corynebacterium glutamicum (CgAK), a bacterium used for industrial fermentation including glutamate inhibited by lysine threonine in concerted manner. To elucidate mechanism this unique regulation CgAK, we determined crystal structures several forms: an inhibitory form complexed with both active only feedback inhibition-resistant...
SUMMARY The nonexpressor of pathogenesis‐related (NPR) gene family is well known to play a crucial role in transactivation TGA transcription factors for salicylic acid (SA)‐responsive genes, including protein 1 ( PR1 ), during plants' immune response after pathogen attack the model dicot Arabidopsis thaliana . However, little about NPR functions monocots. We therefore explored NPRs SA signaling monocot Brachypodium distachyon BdNPR1 and BdNPR2/3 share structural similarities with A. AtNPR1/2...
Natural products containing an aziridine ring, such as mitomycin C and azinomycin B, exhibit antitumor activities by alkylating DNA via their rings; however, the biosynthetic mechanisms underlying formation of these rings have not yet been elucidated. We herein investigated biosynthesis vazabitide A, structure which is similar to that demonstrated Vzb10/11, with no similarities known enzymes, catalyzed ring sulfate elimination. To elucidate detailed reaction mechanism, crystallization...
Abstract The combination of low‐temperature disintegration with gradient and pile centrifugation makes possible the preparation bacterial cell walls under conditions preventing digestion.
To investigate the role of phosphatidylinositol 3-kinase (PI3K) pathway and signal mediator AP-1 in monocyte chemotactic protein (MCP)-1 interleukin (IL)-8 gene expression human retinal pigment epithelial (hRPE) cells.hRPE cells were stimulated with IL-1beta TNF-alpha by coculturing monocytes presence or absence a series kinase inhibitors. The induction MCP-1 IL-8 mRNA was determined ELISA RT-PCR, respectively. Western blot analysis, assays, electrophoretic mobility shift assays used to...
We recently discovered a biosynthetic system using novel amino group carrier protein called LysW for lysine biosynthesis via α-aminoadipate (AAA), and revealed that this is also utilized in the of arginine by Sulfolobus. In present study, we focused on ornithine hyperthermophilic archaeon Thermococcus kodakarensis, showed their accomplished single set metabolic enzymes. determined crystal structure LysX family from T. which catalyzes conjugation with either AAA or glutamate, complex...
We investigated the role of glutamine synthetases (cytosolic GS1 and chloroplast GS2) glutamate synthases (ferredoxin-GOGAT NADH-GOGAT) in inorganic nitrogen assimilation reassimilation into amino acids between bundle sheath cells mesophyll for remobilization during early phase grain filling Zea mays L. The plants responded to a light/dark cycle at level nitrate, ammonium second leaf, upward from primary ear, which acted as source organ. issued distinct pathways acid synthesis were evaluated...