A5030578061- Vector-borne infectious diseases
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Toxin Mechanisms and Immunotoxins
- Microbial infections and disease research
- Vector-Borne Animal Diseases
- Virology and Viral Diseases
- Veterinary Oncology Research
- Antimicrobial Peptides and Activities
- Animal Disease Management and Epidemiology
- Parasite Biology and Host Interactions
- Aquaculture disease management and microbiota
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Rabies epidemiology and control
- Advanced Proteomics Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Cell Adhesion Molecules Research
- Poxvirus research and outbreaks
- Mesenchymal stem cell research
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Identification and Quantification in Food
- Viral Infections and Vectors
- Helminth infection and control
Chiang Mai University
2017-2025
Phuket Rajabhat University
2023
Lumpy skin disease (LSD) is a contagious among cattle and buffalo worldwide. Currently, an enzyme-linked immunosorbent assay (ELISA) has been recognized as efficient diagnostic tool that less time-consuming easier than the viral neutralization test to measure antibody levels. In present study, in-house method of indirect ELISA was developed detect bovine antibodies against virus (LSDV) its performance assessed using field samples. This compared with commercial kit for detection LSDV. The...
Small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) are recognized for their therapeutic potential in immune modulation and tissue repair, especially veterinary medicine. This study introduces an innovative sequential stimulation (IVES) technique, involving low-oxygen gas mixture preconditioning using vitro fertilization (IVFG) direct current electrical (ES20), to enhance the anti-inflammatory properties of sEVs canine adipose-derived MSCs (cAD-MSCs). Initial steps...
Metastasis-related disease is a major cause of death in canine mammary tumours (CMTs). Immunotherapy has been investigated due to the less successful outcomes systemic therapy. This study aims examine expression Programmed Cell Death Ligand-1 (PD-L1) dogs Chiang Mai, Thailand, and determine relationship between level mRNA clinicopathologic characteristics. A total 28 CMT samples were collected at Small Animal Hospital, Mai University. Quantitative reverse transcriptase-polymerase chain...
Duck enteritis virus and Pasteurella multocida are major duck pathogens that induce plague fowl cholera, respectively, in ducks other waterfowl populations, leading to high levels of morbidity mortality. Immunization with live attenuated DEV vaccine containing P. outer membrane protein H (OmpH) can provide the most effective protection against these two infectious diseases ducks. We have recently reported construction recombinant expressing ompH gene using CRISPR/Cas9 editing strategy goal...
Chicken heterophils generate reactive oxygen species (ROS) molecules to defend against invading pathogens. The present study examined effects of quercetin on chicken heterophils. Heterophils were stimulated with PBS, 50 µM (QH), PMA or Escherichia coli (EC) and the resulting intracellular ROS determined. Flow cytometry results showed that cells QH, EC had a higher production. Increases in identified all treatment groups by fluorescence microscopy. Determination ability manipulate mRNA...
Hemorrhagic septicemia (HS) is an important infectious disease in cattle and buffaloes, caused by Pasteurella multocida B:2 E:2. The intranasal recombinant OmpH-based vaccine was successfully used to protect dairy from HS a previous study. Thus, this study aimed examine the protective ability of that among buffaloes. Four groups Thai swamp buffaloes received different vaccines were labeled as 100 or 200 μg rOmpH with CpG-ODN2007, commercial bacterin vaccine, nonvaccinated control groups....
Fowl cholera is a highly contagious disease within the global duck farming industry. This study aimed at formulating and evaluating protective efficacy of combination vaccine containing recombinant outer membrane protein H (rOmpH) Pasteurella multocida strain X-73 with live attenuated plague into single dose. Four groups ducks received different treatments were labelled as non-vaccinated, combined vaccination, vaccination rOmpH respectively. The group was comprised commercial 100 µg to total...
Abstract Background Foot-and-mouth disease (FMD) and Haemorrhagic septicemia (HS) are two important diseases that known to have caused significant economic losses the cattle industry. Accordingly, vaccinations been recognized as an efficient method control prevent both of above-mentioned diseases. This study aimed determine immune response FMD virus antigens recombinant outer membrane protein HS (rOmpH) Pasteurella multocida in administered a combination vaccine compare antibody titers with...
Background Hemoparasites, such as Babesia spp., Theileria spp. and Anaplasma can negatively affect the health of farm animals resulting in significant losses production. These inherently economics livestock industry. Since increases severity vector-borne diseases southeast Asian region have been reported, investigations parasitic epidemiology Thailand will be necessary to improve existing parasite control strategies for blood infections. This study aims investigate incidences bovine...
Fowl cholera is caused by the bacterium Pasteurella multocida, a highly transmissible avian ailment with significant global implications, leading to substantial economic repercussions. The control of fowl outbreaks primarily relies on vaccination using traditional vaccines that are still in use today despite their many limitations. In this research, we describe development genetically engineered herpesvirus turkeys (HVT) carries OmpH gene from P. multocida integrated into UL 45/46 intergenic...
Canine mammary tumours (CMTs) are regarded as invasive with a high rate of recurrent and metastasis in intact female dogs. Tumour diagnosis, therefore, is an important step predicting monitoring tumour progression. This study was designed to identify protein expression on CMTs by employing proteomic approach. The primary cell culture from benign mixed tumour, simple carcinoma, complex carcinoma normal gland were established, two-dimensional electrophoresis (2DE) subsequently performed....
Both strong innate and adaptive immune responses are an important component of protection against intraerythrocytic protozoan parasites. Resistance to bovine babesiosis is associated with interferon (IFN)-γ mediated responses. CD4 + T cells macrophages have been identified as major effector mediating the clearance pathogens. Previously, apical membrane antigen 1 (AMA-1) was found significantly induce response inhibiting B. bovis merozoite growth invasion. However, a detailed characterization...
Bovine babesiosis caused by Babesia bovis (B. bovis) has had a significant effect on the mobility and mortality rates of cattle industry worldwide. Live-attenuated vaccines are currently being used in many endemic countries, but their wide use been limited for number reasons. Although recombinant have proposed as an alternative to live vaccines, such not commercially available date. Apical membrane antigen-1 (AMA-1) is one leading candidates development vaccine against diseases apicomplexan...
Abstract Background Feline panleukopenia virus (FPV) is an etiologic pathogen of feline that infects all members Felidae including tigers ( Panthera tigris ). Vaccinations against FPV among wild felid species have long been practiced in zoos worldwide. However, few studies assessed the tiger immune response post-vaccination due to absence a serological diagnostic tool. To address these limitations, this study aimed develop in-house indirect enzyme-linked immunosorbent assay (ELISA) for...
Melanoma-associated antigen-A (MAGE-A), a family of cancer/testis antigens, has been recognized as potential target molecule for cancer immunotherapy. However, there very little information available with regard to this antigen in dogs. This study aimed investigate the expression MAGE-A canine mammary gland tumors (CMTs) using immunohistochemistry and immunoblotting human monoclonal antibody 6C1. The present provided evidence cross-reactivity CMTs. antigens were expressed moderate-...
In terms of its veterinary importance, vaccine development against Ehrlichia canis is needed. However, the effect developing vaccines on humoral immune response E. infection still unknown. Novel GP194-43 was synthesized according to GP19 epitope prediction. To restrict any loss and/or illness in host animal, rabbits were used this study produce hyperimmune sera. The sera neutralization examined vitro by determining inhibition macrophage-like cell line (DH82) presence Four groups DH82 cells...
The intracellular bacterium Ehrlichia canis is the causative pathogen of canine monocytic ehrlichiosis (CME) in dogs. Despite its veterinary and medical importance, there currently no available vaccine against this pathogen. In study, recombinant GP19 (rGP19) was produced used as a prototype mouse model experimental E. infection. efficacy rGP19 part stimulating B T cell responses conferring protection mice later challenged with were evaluated. rGP19-specific antibody response evaluated by...
Abstract Background: Feline panleukopenia virus (FPV) is an etiologic pathogen of feline that infects all members Felidae including tigers ( Panthera tigris ). Vaccination against FPV among wild felid species has long been practiced in zoos worldwide. However, few studies have assessed tiger immune response post-vaccination due to the absence a serological diagnostic tool. To address these limitations, this study aimed develop in-house indirect enzyme-linked immunosorbent assay (ELISA) for...
A captive map pufferfish from Phuket Sea region presented with abnormal posture of swimming and breathing. The veterinarian found ectoparasites on the puffer’s fins skin. With morphology under stereomicroscope, Caligus fugu was identified. previously limited information, this report introduced first evidence C. infestation in pufferfish, Thailand. puffer successfully treated 0.0075% hydrogen peroxide against adult negative for parasitic organisms. importance aquaculture, especially aquariums...
Abstract Background Bovine babesiosis caused by Babesia bovis ( B. ) affects the cattle industry worldwide with high mobility and mortality. Live-attenuated vaccines are currently used in some of endemic countries, but their wide use is limited due to various reasons. Although recombinant have been proposed as an alternative live vaccines, such not commercially available date. Apical membrane antigen-1 (AMA-1) one leading candidates for vaccine development against diseases apicomplexan...