A5025478649- Malaria Research and Control
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Herpesvirus Infections and Treatments
- Toxin Mechanisms and Immunotoxins
- Virology and Viral Diseases
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Viral Infections and Vectors
- HIV/AIDS drug development and treatment
- Sulfur Compounds in Biology
- Research on Leishmaniasis Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Viral Infections and Outbreaks Research
- Insect Pest Control Strategies
- Traditional and Medicinal Uses of Annonaceae
- Virus-based gene therapy research
- Poxvirus research and outbreaks
- Microbial infections and disease research
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
Chiang Mai University
2021-2025
National Center for Genetic Engineering and Biotechnology
2016-2020
National Science and Technology Development Agency
2015-2020
Lumpy skin disease (LSD) is a contagious among cattle and buffalo worldwide. Currently, an enzyme-linked immunosorbent assay (ELISA) has been recognized as efficient diagnostic tool that less time-consuming easier than the viral neutralization test to measure antibody levels. In present study, in-house method of indirect ELISA was developed detect bovine antibodies against virus (LSDV) its performance assessed using field samples. This compared with commercial kit for detection LSDV. The...
The melanoma-associated antigen gene (MAGE) is a key target in cancer immunotherapy. Given the potential of MAGE-B genes veterinary immunotherapy for canine mammary tumors (CMTs), this study investigated mRNA expression MAGE-B1, -B4, -B5, and -B10 CMT tissues cells from dogs. Quantitative real-time PCR was used to analyze 28 tissue samples, including 4 benign 24 malignant (13 simple carcinomas, 6 complex 3 carcinosarcomas, 2 fibrosarcomas). Benign mixed tumor carcinoma-type were cultured...
Metastasis-related disease is a major cause of death in canine mammary tumours (CMTs). Immunotherapy has been investigated due to the less successful outcomes systemic therapy. This study aims examine expression Programmed Cell Death Ligand-1 (PD-L1) dogs Chiang Mai, Thailand, and determine relationship between level mRNA clinicopathologic characteristics. A total 28 CMT samples were collected at Small Animal Hospital, Mai University. Quantitative reverse transcriptase-polymerase chain...
Control of malaria is threatened by emerging parasite resistance to artemisinin and derivative drug (ART) therapies. The molecular detail how Plasmodium parasites respond ART this could contribute are not well understood. To address question, we performed a transcriptomic study dihydroartemisinin (DHA) response in P. falciparum K1 strain berghei ANKA using microarray RNA-seq technology. Microarray data from DHA-treated trophozoite stage revealed pattern that overall less trophozoite-like...
Duck enteritis virus and Pasteurella multocida are major duck pathogens that induce plague fowl cholera, respectively, in ducks other waterfowl populations, leading to high levels of morbidity mortality. Immunization with live attenuated DEV vaccine containing P. outer membrane protein H (OmpH) can provide the most effective protection against these two infectious diseases ducks. We have recently reported construction recombinant expressing ompH gene using CRISPR/Cas9 editing strategy goal...
Background Hemoparasites, such as Babesia spp., Theileria spp. and Anaplasma can negatively affect the health of farm animals resulting in significant losses production. These inherently economics livestock industry. Since increases severity vector-borne diseases southeast Asian region have been reported, investigations parasitic epidemiology Thailand will be necessary to improve existing parasite control strategies for blood infections. This study aims investigate incidences bovine...
Vesicular stomatitis virus (VSV) is highly immunogenic and able to stimulate both innate adaptive immune responses. However, its ability induce adverse effects has held back the use of VSV as a potential vaccine vector. In this study we developed VSV-ΔP, safe yet potent replication-defective recombinant in which phosphoprotein (P) gene was deleted. VSV-ΔP replicated only supporting cells expressing P (BHK-P cells) at levels more than 2 logs lower VSV. vivo studies indicated that moderate...
Glutathione plays a central role in maintaining cellular redox homeostasis, and modulations to this status may affect malaria parasite sensitivity certain types of antimalarials. In study, we demonstrate that inhibition glutathione biosynthesis the Plasmodium berghei ANKA strain through disruption γ-glutamylcysteine synthetase (γ-GCS) gene, which encodes first rate-limiting enzyme biosynthetic pathway, significantly sensitizes parasites vivo pyrimethamine sulfadoxine, but not chloroquine,...
Fowl cholera is caused by the bacterium Pasteurella multocida, a highly transmissible avian ailment with significant global implications, leading to substantial economic repercussions. The control of fowl outbreaks primarily relies on vaccination using traditional vaccines that are still in use today despite their many limitations. In this research, we describe development genetically engineered herpesvirus turkeys (HVT) carries OmpH gene from P. multocida integrated into UL 45/46 intergenic...
Both strong innate and adaptive immune responses are an important component of protection against intraerythrocytic protozoan parasites. Resistance to bovine babesiosis is associated with interferon (IFN)-γ mediated responses. CD4 + T cells macrophages have been identified as major effector mediating the clearance pathogens. Previously, apical membrane antigen 1 (AMA-1) was found significantly induce response inhibiting B. bovis merozoite growth invasion. However, a detailed characterization...
In terms of its veterinary importance, vaccine development against Ehrlichia canis is needed. However, the effect developing vaccines on humoral immune response E. infection still unknown. Novel GP194-43 was synthesized according to GP19 epitope prediction. To restrict any loss and/or illness in host animal, rabbits were used this study produce hyperimmune sera. The sera neutralization examined vitro by determining inhibition macrophage-like cell line (DH82) presence Four groups DH82 cells...
The intracellular bacterium Ehrlichia canis is the causative pathogen of canine monocytic ehrlichiosis (CME) in dogs. Despite its veterinary and medical importance, there currently no available vaccine against this pathogen. In study, recombinant GP19 (rGP19) was produced used as a prototype mouse model experimental E. infection. efficacy rGP19 part stimulating B T cell responses conferring protection mice later challenged with were evaluated. rGP19-specific antibody response evaluated by...
Abstract Inhibitors for Plasmodium falciparum dihydrofolate reductase (DHFR) form an important class of antimalarial drugs widely used malaria treatment, but have been compromised by development resistance to the drugs. Mutations in DHFR are main contributing factors resistance. Although new, rationally designed antifolates active against resistant P. , such as P218, developed, activity quadruple mutant (V1/S) has only demonstrated vitro and vivo shown SCID mice. A convenient model...
The current first line drugs for treating uncomplicated malaria are artemisinin (ART) combination therapies. However, Plasmodium falciparum parasites resistant to ART and partner spreading, which threatens control efforts. Rodent species useful models understanding antimalarial resistance, in particular genetic variants responsible cross resistance different compounds. berghei RC strain (PbRC) is described as antimalarials, including chloroquine (CQ) ART. In an attempt identify the basis...