A5081837903- Malaria Research and Control
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
- Chemical Synthesis and Analysis
- vaccines and immunoinformatics approaches
- Invertebrate Immune Response Mechanisms
- Research on Leishmaniasis Studies
- Computational Drug Discovery Methods
- Synthesis and Catalytic Reactions
- Monoclonal and Polyclonal Antibodies Research
- Polyamine Metabolism and Applications
- Insect Resistance and Genetics
National Science and Technology Development Agency
2011-2022
National Center for Genetic Engineering and Biotechnology
2011-2022
Conventional reverse genetic approaches for study of Plasmodium malaria parasite gene function are limited, or not applicable. Hence, new inducible systems needed. Here we describe a method to control P. falciparum expression in which target genes bearing glmS ribozyme the 3' untranslated region efficiently knocked down transgenic parasites response glucosamine inducer. Using reporter genes, show that cleaves mRNA vivo leading reduction following treatment. Glucosamine-induced activation led...
Control of malaria is threatened by emerging parasite resistance to artemisinin and derivative drug (ART) therapies. The molecular detail how Plasmodium parasites respond ART this could contribute are not well understood. To address question, we performed a transcriptomic study dihydroartemisinin (DHA) response in P. falciparum K1 strain berghei ANKA using microarray RNA-seq technology. Microarray data from DHA-treated trophozoite stage revealed pattern that overall less trophozoite-like...
The S108N mutation of dihydrofolate reductase (DHFR) renders Plasmodium falciparum malaria parasites resistant to pyrimethamine through steric clash with the rigid side chain inhibitor. Inhibitors flexible chains can avoid this and retain effectiveness against mutant. However, other mutations such as N108S reversion confer resistance inhibitors. We designed synthesized hybrid inhibitors two structural types in a single molecule, which are effective both wild-type multiple mutants P. their...
Abstract Background Pyronaridine (PN) and chloroquine (CQ) are structurally related anti-malarial drugs with primarily the same mode of action. However, PN is effective against several multidrug-resistant lines Plasmodium falciparum , including CQ resistant lines, suggestive important operational differences between two drugs. Methods Synchronized trophozoite stage cultures P. strain K1 (CQ resistant) were exposed to 50% inhibitory concentrations (IC 50 ) CQ, parasites harvested from culture...
The Plasmodium falciparum human malaria parasite genome is incompletely annotated and does not accurately represent the transcriptomic diversity of this species. To address need, we performed long-read sequencing. 5′ capped mRNA was enriched from samples total nuclear-fractionated RNA intra-erythrocytic stages converted to cDNA library. libraries were sequenced on PacBio Nanopore platforms. 12,495 novel isoforms data. Alternative 3′ ends majority isoform events among isoforms, with retained...
The genome of the human malaria parasite Plasmodium falciparum is poorly annotated, in particular, 5' capped ends its mRNA transcripts. New approaches are needed to fully catalog P. transcripts for understanding gene function and regulation this organism.We developed a transcriptomic method based on next-generation sequencing complementary DNA (cDNA) enriched full-length fragments using eIF4E, cap-binding protein, an unenriched control. adapter was added after enrichment cDNA two different...
The treatment of a variety protozoal infections, in particular those causing disabling human diseases, is still hampered by lack drugs or increasing resistance to registered drugs. However, recent years, remarkable progress has been achieved combat neglected tropical diseases sequencing the parasites’ genomes validation new targets parasites novel genetic manipulation techniques, leading loss function. amino acid hypusine posttranslational modification (PTM) that occurs eukaryotic initiation...