A5058488477- Malaria Research and Control
- Mosquito-borne diseases and control
- Burkholderia infections and melioidosis
- Chemical Looping and Thermochemical Processes
- Brucella: diagnosis, epidemiology, treatment
- Complement system in diseases
- Subcritical and Supercritical Water Processes
- MicroRNA in disease regulation
- Travel-related health issues
- HIV/AIDS drug development and treatment
- Cancer-related molecular mechanisms research
- Digital Imaging for Blood Diseases
- Circular RNAs in diseases
- Vector-borne infectious diseases
- Aquaculture disease management and microbiota
- vaccines and immunoinformatics approaches
- Computational Drug Discovery Methods
- Viral Infectious Diseases and Gene Expression in Insects
- Hepatitis Viruses Studies and Epidemiology
Rangsit University
2011-2025
Mahidol University
2011
National Science and Technology Development Agency
2011
National Center for Genetic Engineering and Biotechnology
2011
Acinetobacter baumannii is a priority bacterial pathogen and leading cause of nosocomial infections, particularly in intensive care units (ICUs). The average incidence carbapenem-resistant A. infections ICUs 41.7 cases/1,000 patients, highlighting the urgent need for more effective alternative therapies to replace carbapenems. Thus, this study aimed investigate first time antibacterial activity curcumin combination with novel phage vB_AbaSI_1 combat multidrug-resistant (MDR) vitro. Phage...
To determine the prevalence of malaria infections among foreign migrant workers in Thailand. Giemsa-stained thin and thick blood films were prepared from samples 294 recruited study. Microscopic examination these was performed for detection. Blood film revealed 1.36% subjects. All positive cases male Myanmar which their only ring stage Plasmodium spp. found at low parasite density (mean= 144 parasites/μ L blood). The not significantly different classified by age, gender, resident province...
Abstract Burkholderia pseudomallei is a saprophytic soil bacterium and the etiological agent that causes melioidosis. It naturally resistant to many antibiotics therefore difficult treat. Bacteriophages may provide an alternative source of treatment. We have isolated characterised bacteriophage ΦBp-AMP1. The phage member Podoviridae family has genome size ~ 45 Kb. Molecular data based on gene which encodes for tail tubular protein suggests distinct from known phages but related infect B....
Abstract Background Pyronaridine (PN) and chloroquine (CQ) are structurally related anti-malarial drugs with primarily the same mode of action. However, PN is effective against several multidrug-resistant lines Plasmodium falciparum , including CQ resistant lines, suggestive important operational differences between two drugs. Methods Synchronized trophozoite stage cultures P. strain K1 (CQ resistant) were exposed to 50% inhibitory concentrations (IC 50 ) CQ, parasites harvested from culture...
Abstract Plasmodium vivax is the most prevalent cause of malaria in Thailand and predominant malarial endemic areas worldwide. P. infection characterized by low parasitemia, latent liver-stage parasites, or asymptomatic infections leading to underreported cases. These are significant challenges for controlling eliminating from countries. This study developed evaluated a dot-blot enzyme-linked immunosorbent assay (ELISA) using PvMSP1-42 recombinant antigen serological diagnosis based on...
Considering the importance of merozoite surface proteins (MSPs) as vaccine candidates, this study was conducted to investigate polymorphism and genetic diversity Plasmodium vivax protein 3-alpha (PvMSP-3α) in Thailand. To analyze diversity, 118 blood samples containing P. were collected from four malaria-endemic areas western southern The DNA extracted amplified for PvMSP-3α gene using nested PCR. PCR products genotyped by PCR-RFLP with Hha I Alu restriction enzymes. combination patterns...
Melioidosis caused by gram negative bacteria, B. pseudomallei, is a fatal disease in the tropical and sub-tropical regions. However, sporadic cases have been reported elsewhere. Early detection imperative to reduce mortality rate. Serological tests being substantially developed using recombinant proteins as specific targeted antigens melioidosis antibodies. In present study, we focus on truncated flagellin fragment (FLAG300) outer membrane protein A (OmpABT) of thailandensis E264 potential...
MiR27a plays an important role in carcinogenesis, cell proliferation, apoptosis, invasion, migration and angiogenesis. Several studies have identified of pre-miR27a (rs895819) A>G polymorphism several types cancer. This research aims to investigate the association breast cancer susceptibility, clinicopathological data survival. Blood DNA samples 143 Thai patients 100 healthy women were studied for using polymerase chain reaction-restriction fragment-length (PCR-RFLP).The results revealed...
Abstract Background The accurate and rapid diagnosis of melioidosis is challenging. Several serological approaches have been developed using recombinant antigens to improve the diagnostic indices tests for melioidosis. Methods Fusion proteins from Burkholderia pseudomallei (rGroEL-FLAG300) were evaluated as a potential target antigen antibodies. A total 220 serum samples 38 culture proven patients (gold standard), 126 healthy individuals endemic (n = 37) non-endemic 89) Thai provinces 56...
Background: Melioidosis is a disease caused by the Burkholderia pseudomallei bacterium. The mortality rate of infected patients quite high because symptoms are similar to those various diseases, making it difficult diagnose clinically and preventing immediate treatment with effective antibiotics that required for management acute infections. To provide appropriate treatment, accurate rapid diagnosis required. Objective: aims this study were develop Dot ELISA using purified GroEL B....
Objective: The majority of malaria cases and deaths are caused by Plasmodium falciparum. rapid accurate diagnosis is very important for treatment control. aim this study was to evaluate two nested polymerase chain reaction (PCR)-based methods (protocol A protocol B) P. falciparum infection, in Thailand.Methods: total 90 dried blood spot samples were investigated. composed falciparum-, vivax-infected normal human samples. microscopic examination used as gold standard.Results: results showed...
Genetic diversity of Plasmodium parasite has significantly related to malaria control and vaccine development. The P. falciparum merozoite surface protein 1 ( Pfmsp1 ) gene is a commonly used molecular marker differentiate genetic diversity. This study aimed at developing nested PCR-Heteroduplex Mobility Assay (nPCR-HMA) for determination the block 2 gene. MAD20 family allele was as optimization annealing polyacrylamide gel electrophoresis conditions. In order evaluate developed nPCR-HMA, 8...