A5031764318- Leptospirosis research and findings
- Antifungal resistance and susceptibility
- Viral Infections and Vectors
- Malaria Research and Control
- Antibiotic Resistance in Bacteria
- Fungal Infections and Studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Molecular Communication and Nanonetworks
- Neonatal and Maternal Infections
- Chemical Looping and Thermochemical Processes
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV/AIDS drug development and treatment
- Pharmaceutical and Antibiotic Environmental Impacts
- Computational Drug Discovery Methods
- Burkholderia infections and melioidosis
- Bacteriophages and microbial interactions
- Legionella and Acanthamoeba research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Zoonotic diseases and public health
- Yeasts and Rust Fungi Studies
- Antibiotics Pharmacokinetics and Efficacy
- Nanoplatforms for cancer theranostics
Vajira Hospital
2017-2024
Navamindradhiraj University
2017-2024
Mahidol University
2010-2013
Mahidol Oxford Tropical Medicine Research Unit
2011-2013
Churchill Hospital
2013
Addenbrooke's Hospital
2011-2013
University of Cambridge
2011-2013
University of Oxford
2011-2013
Département Santé Animale
2011
United Nations
2011
The available Leptospira multilocus sequence typing (MLST) scheme supported by a MLST website is limited to L. interrogans and kirschneri. Our aim was broaden the utility of this incorporate total seven pathogenic species.We modified existing replacing one loci (fadD changed caiB), as former gene did not appear be present in some species. Comparison original schemes using data for kirschneri demonstrated that discriminatory power two significantly different. used further characterize 325...
Global leptospirosis disease burden estimates are hampered by the lack of scientifically sound data from countries with probable high endemicity and limited diagnostic capacities. We describe seroepidemiologic clinical characteristics outbreak in 2008 Sri Lanka. Definitive/presumptive case definitions proposed World Health Organization Leptospirosis Epidemiology Reference Group were used for confirmation. Of 404 possible cases, 155 confirmed to have leptospirosis. Highest titers patient seum...
Candidemia is often associated with high mortality, and Candida albicans, tropicalis, glabrata, parapsilosis are common causes of this disease. The pathogenicity characteristics specific spp. that cause candidemia in Thailand poorly understood. This study aimed to characterize the virulence factors Thirty-eight isolates different species from blood cultures were evaluated for their properties, including exoenzyme biofilm production, cell surface hydrophobicity, tissue invasion, epithelial...
There is a lack of diagnostic tests for leptospirosis in technology-restricted settings. We developed loop-mediated isothermal amplification (LAMP) specific the 16S ribosomal RNA gene (rrs) pathogenic and intermediate group Leptospira species. The lower limit detection was 10 genomic equivalents/reaction, analytical specificity high; we observed positive reactions pathogenic/intermediate groups negative non-pathogenic species other bacterial evaluated this assay Thailand by using...
Background Melioidosis is a severe disease caused by Burkholderia pseudomallei. Clinical manifestations are diverse and acute infections require immediate treatment with effective antibiotics. While culture the current diagnostic standard, it time-consuming has low sensitivity. In endemic areas, inaccessibility to biosafety level 3 facilities lack of good serodiagnostic tools can impede diagnosis surveillance. Recent studies have suggested that O-polysaccharide (OPS) hemolysin co-regulated...
Abstract Background Pathogenic Leptospira spp. present in the blood of patients with leptospirosis during first week symptoms can be detected using culture or PCR. A proportion who are positive by PCR negative culture. fastidious bacteria, and we hypothesized that a false-negative result may represent infection distinct bacterial subset fail to grow standard medium. Methods We evaluated our hypothesis prospective study 418 consecutive presenting hospital northeast Thailand an acute febrile...
Background Several sequence based genotyping schemes have been developed for Leptospira spp. The objective of this study was to genotype a collection clinical and reference isolates using the two most commonly used compare contrast results. Methods Findings A total 48 consisting L. interrogans (n = 40) kirschneri 8) were typed by 7 locus MLST scheme described Thaipadungpanit et al., 6 Ahmed (termed 7L 6L, respectively). Two not 6L because deletion three nucleotides in lipL32. remaining 46...
Candida species represent a common cause of bloodstream infection (BSI). Given the emergence non-albicans (NAC) associated with treatment failure, investigations into distribution, fungal susceptibility profile, and molecular epidemiology pathogens are necessary to optimize candidemia explore transmission drug resistance for control management. This study evaluated prevalence, antifungal susceptibility, characteristics causing BSI in tertiary-level hospital Bangkok, Thailand. In total, 54...
Floodwater samples (N = 110) collected during the 2011 Bangkok floods were tested for Leptospira using culture and polymerase chain reaction (PCR); 65 PCR-positive putatively non-pathogenic species, 1 sample contained a pathogenic Leptospira, 6 clustering phylogenetically with intermediate group. The low prevalence of in floodwater was consistent number human leptospirosis cases reported to Bureau Epidemiology Thailand. This study provides baseline information on environmental together set...
Acinetobacter baumannii is an opportunistic gram-negative bacteria typically attributed to hospital-associated infection. It could also become multidrug-resistant (MDR), extensively drug-resistant (XDR), and pan (PDR) during a short period. Although A . has been documented extensively, complete knowledge on the antibiotic-resistant mechanisms virulence factors responsible for pathogenesis not entirely elucidated. This study investigated drug resistance pattern characterized genomic sequence...
Abstract Plasmodium vivax is the most prevalent cause of malaria in Thailand and predominant malarial endemic areas worldwide. P. infection characterized by low parasitemia, latent liver-stage parasites, or asymptomatic infections leading to underreported cases. These are significant challenges for controlling eliminating from countries. This study developed evaluated a dot-blot enzyme-linked immunosorbent assay (ELISA) using PvMSP1-42 recombinant antigen serological diagnosis based on...
Abstract Tigecycline-resistant Acinetobacter baumannii (TRAB) is increasing in Thailand, complicating antibiotic treatment due to limited options. The specific resistance mechanism behind tigecycline still unclear, necessitating further investigation. We investigated the presence of OXA-type carbapenemases, antimicrobial susceptibility profile, inhibitory effect carbonyl cyanide m-chlorophenylhydrazone (CCCP) on susceptibility, expression levels RND-type efflux pumps and amino acid...
The basidiomycetes yeast Trichosporon is widespread in the natural environment, but can cause disease, mainly immunocompromised patients. However, there have been only few studies about this infection Thailand. In study, we characterized 53 spp. isolated from urine samples patients admitted to a single hospital Bangkok, Thailand over one-year period 2019 2020. strains were identified using colony morphology, microscopy, matrix-assisted laser desorption/ionization time-of-flight mass...
The urgent need for an effective COVID-19 therapy has propelled the exploration of innovative strategies to combat fast-mutating SARS-CoV-2 virus. This study attempted develop nanobodies (Nbs) against Omicron variants by redirecting 1.29 neutralizing Nb, a receptor-binding domain (RBD)-specific Nb that can protect various other than Omicron, target subvariant BA.5, variant used development bivalent vaccine. Error-prone libraries were constructed. Following two rounds selection using...
Little is known about the properties of current strains Staphylococcus aureus associated with human infections in Thailand. This study examined rate resistance to various antimicrobial agents, prevalence virulence genes, and biofilm formation ability 60 clinical S. isolates from a single Thai hospital. Moreover, protein A gene (spa) type was determined among methicillin-resistant (MRSA) isolates. Most methicillin-susceptible were susceptible antimicrobials, whereas all MRSA resistant...
The emergence in Southeast Asia of invasive group B Streptococcus (GBS) infections adults by sequence type (ST) 283 is suggested to be associated with fish consumption. Genotyping 55 GBS clinical isolates revealed that 33/44 belonged ST283/capsular polysaccharide (CPS) III. This included 15/16 recovered from younger aged 16-36 years. Seven ST283/CPSIII the blood, cerebrospinal fluid, or joint fluid were selected patient's age at random perform interaction studies intestinal epithelial Caco-2...