A5062579810- Leptospirosis research and findings
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Malaria Research and Control
- Vector-borne infectious diseases
- Escherichia coli research studies
- Mollusks and Parasites Studies
- Parasite Biology and Host Interactions
- Complement system in diseases
- Parasitic Infections and Diagnostics
- Burkholderia infections and melioidosis
- Identification and Quantification in Food
- Parasites and Host Interactions
- Helminth infection and control
- Veterinary medicine and infectious diseases
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Pneumonia and Respiratory Infections
- Brucella: diagnosis, epidemiology, treatment
- Antifungal resistance and susceptibility
- Plant Pathogens and Resistance
- Antibiotic Resistance in Bacteria
- Monoclonal and Polyclonal Antibodies Research
Mahidol Oxford Tropical Medicine Research Unit
2008-2022
Mahidol University
2010-2022
Queensland Health
2011
Addenbrooke's Hospital
2011
United Nations
2011
Ministry of Public Health
2011
Royal Tropical Institute
2011
Département Santé Animale
2011
University of Cambridge
2011
University of Oxford
2011
A single Leptospira strain (designated Khorat-H2T) was isolated from the urine of an adult male patient with suspected leptospirosis province Nakornrachasima, Thailand. The isolate showed typical motility and morphology under dark-field microscopy. Cells were 10–13 μm long 0.2 in diameter, a wavelength 0.5 amplitude approximately 0.3 μm. Phenotypically, Khorat-H2T did not grow at 13 °C but grew 30 37 presence 8-azaguanine. Serological identification using microscopic agglutination test...
Orientia tsutsugamushi is the causative agent of scrub typhus, a major cause febrile illness in rural areas Southeast Asia. Twenty-three strains O. were isolated from patients with typhus north-east (Udorn Thani province) and western Thailand (Tak between 2003 2005. The isolates characterized by sequencing entire ORF 56-kDa-type-specific antigen gene, followed phylogenetic analysis. majority (15/23) clustered Karp-type strain, six Gilliam-type strain one each TA716- TA763-type strains....
There is a lack of diagnostic tests for leptospirosis in technology-restricted settings. We developed loop-mediated isothermal amplification (LAMP) specific the 16S ribosomal RNA gene (rrs) pathogenic and intermediate group Leptospira species. The lower limit detection was 10 genomic equivalents/reaction, analytical specificity high; we observed positive reactions pathogenic/intermediate groups negative non-pathogenic species other bacterial evaluated this assay Thailand by using...
ABSTRACT Hybridomas secreting specific monoclonal antibodies (MAb) to all members of the genus Leptospira (clone LF9) and those that are only pathogenic species (clones LD5 LE1) were produced. MAb LF9, which was immunoglobulin G1 (IgG1), reacted a 38-kDa component sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated whole-cell lysates spp., while LE1, IgG1 IgG2a, respectively, 35- 36-kDa components serogroups Leptospira. The used in dot blot-enzyme-linked immunosorbent assay...
Cytochrome P450 2B6 (CYP2B6) metabolizes efavirenz and nevirapine, the major core antiretroviral drugs for HIV in Thailand. Rifampicin, a critical component of tuberculosis (TB) therapy is potent inducer CYP enzyme activity. Polymorphisms CYP2B6 CYP3A4 are associated with altered activity hepatic liver pharmacokinetics resulting treatment efficacy. This study aimed to investigate whether or polymorphisms had effects on plasma nevirapine concentrations when co-administered rifampicin HIV/TB...
The outer membrane protein LipL21, LipL32, LipL41 and Loa22 of Leptospira interrogans serovar Copenhageni were previously revealed by immunoproteomic analysis, using sera from acute phase infection in a guinea pig. full-length DNA each was then cloned the same expressed pRSET vector. obtained molecular weight (MW) recombinant proteins rLipL21, rLipL32 rLoa22 slightly higher than MW predicted nucleotide sequences inserted gene, while only N-terminal half rLipL41 obtained. Mice antiserum...
Although Leptospira borgpetersenii subtype Hardjobovis and L. interrogans Hardjoprajitno belong to different species, they are serologically indistinguishable therefore classified as serovar Hardjo. Since LPS is the major antigen involved in serological classification, this implies that of these subtypes identical. Comparison biosynthetic loci (rfb) revealed remarkable similarity, with 32 31 origins replication (orfs) rfb loci, respectively. The order orientation orfs were identical...
Rapid and reliable detection of non-typhoidal Salmonella (NTS) is essential for effective monitoring controlling in broiler industries. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a sensitive accurate method microbial investigations at genus species level while subspecies still obscure. Here, we developed MALDI-TOF MS-based to improve the simultaneous identification species, subspecies, serovars NTS isolated from samples...
Monoclonal antibodies were raised against Barber antigen (Ba) of Salmonella typhi 0901. Antibodies produced to 9 group D salmonellae used in double- and triple-sandwich antibody enzyme-linked immunosorbent assays (ELISAs) for detecting urine plasma specimens from three groups patients 49 controls. The triple-antibody ELISA detected the samples 11 18 (65%) with hemoculture-proven typhoid (group 1) 12 39 (31%) clinical features compatible but whose hemocultures negative 2). This was whom...
Current data on risk of travelers' diarrhea (TD) among visitors to Thailand largely comes from US military personnel, Peace Corps volunteers, or expatriates. We performed a 14‐month systematic study the incidence rate and characteristics TD smaller etiology disease Phuket Chiang Mai. One randomly selected day each week August 2005 until October 2006, were collected foreign tourists departing airports serving A separate subgroup subjects with acquired in invited submit stool sample for...
Antimicrobial peptides are promising molecules to address the global antibiotic resistance problem, however, optimization achieve favorable potency and safety is required. Here, a peptide-template modification approach was employed design physicochemical variants based on net charge, hydrophobicity, enantiomer, terminal group. All of scorpion venom peptide BmKn-2 with amphipathic α-helical cationic structure exhibited an increased antibacterial when evaluated against multidrug-resistant...
Rosetting and cytoadherence of Plasmodium falciparum-infected red blood cells have been associated with severity malaria. ICAM-1 CD36 are the main host cell receptors, while PfEMP1-DBLalpha is a major parasite ligand, which can contribute to rosette formation. This study aimed at demonstrating whether highly polymorphic sequences occurring among Thai isolates causing severe uncomplicated malaria their ability form rosettes reflected clinical outcome patients.Two hundred ninety five from were...
Abstract Background The IL4 -590 gene polymorphism has been shown to be associated with elevated levels of anti- Plasmodium falciparum IgG antibodies and parasite intensity in the malaria protected Fulani West Africa. This study aimed investigate possible impact -590C/T on P. subclasses IgE alteration severity complicated uncomplicated patients or without previous experiences. Methods Anti- P.falciparum plasma experiences were analysed using ELISA. polymorphisms genotyped RFLP-PCR....
The oomycete organism, Pythium insidiosum, is the etiologic agent of life-threatening infectious disease called "pythiosis". Diagnosis and treatment pythiosis difficult challenging. Novel methods for early diagnosis effective are urgently needed. Recently, we reported a 74-kDa immunodominant protein P. which could be diagnostic target, vaccine candidate, virulence factor. was identified as putative exo-1,3-ß-glucanase (Exo1). This study reports on genetic, immunological, biochemical...
Third (infective)-stage Gnathostoma spinigerum larvae (L3) mainly cause human gnathostomiasis. G. L3 migrate throughout the subcutaneous tissues, vital organs, and central nervous system can various pathogenesis including sudden death. Interestingly, survive evade host cellular immunity for months or years. The effects of excretory-secretory (ES) products involved in larval migration immune-evasive strategies are unknown. Monocytes innate immune cells that act as phagocytic...
ABSTRACT Lipopolysaccharide (LPS) is a key antigen in immunity to leptospirosis. Its biosynthesis requires enzymes for the and polymerization of nucleotide sugars transport through attachment bacterial membrane. The genes encoding these functions are commonly clustered into loci; Leptospira borgpetersenii serovar Hardjo subtype Hardjobovis, this locus, named rfb , spans 36.7 kb contains 31 open reading frames, which 28 have been assigned putative on basis sequence similarity....
Paragonimus heterotremus is the main causative agent of paragonimiasis in Thailand. In Western blot diagnostic assays for paragonimiasis, 35 kDa band present crude P. somatic extracts represents one known bands. This study aimed to use a cDNA library create recombinant version this antigen immunodiagnosis paragonimiasis. To accomplish aim expression was constructed from adult worm mRNA and immuno-screened using antibodies mice that had been immunized with antigen. Screening resulted...