A5104289917- Leptospirosis research and findings
- Advanced biosensing and bioanalysis techniques
- Viral Infections and Vectors
- Veterinary medicine and infectious diseases
- RNA Interference and Gene Delivery
- Crop Yield and Soil Fertility
- DNA and Nucleic Acid Chemistry
- Insect Pest Control Strategies
- RNA modifications and cancer
- Viral gastroenteritis research and epidemiology
- Insect-Plant Interactions and Control
- RNA and protein synthesis mechanisms
- Insect and Pesticide Research
- Click Chemistry and Applications
- Salmonella and Campylobacter epidemiology
- Soil Carbon and Nitrogen Dynamics
- Sugarcane Cultivation and Processing
- Pharmacological Effects and Assays
- Cancer Genomics and Diagnostics
- Liver Disease Diagnosis and Treatment
- Genomics and Phylogenetic Studies
- Reproductive Physiology in Livestock
- Soil and Unsaturated Flow
- Genetic and phenotypic traits in livestock
- Animal Diversity and Health Studies
Assiut University
2012-2024
University of Khartoum
2024
London Cancer
2024
Master's College
2024
Cleveland Clinic
2024
University of Amsterdam
2019-2023
Amsterdam University Medical Centers
2020-2023
Benha University
2022-2023
University College London
2022-2023
Agricultural Research Center
2016-2023
Available serological diagnostics do not allow the confirmation of clinically suspected leptospirosis at early acute phase illness. Several conventional and real-time PCRs for diagnosis have been described but these incompletely evaluated. We developed a SYBR Green-based PCR targeting secY validated it according to international guidelines. To determine analytical specificity, DNA from 56 Leptospira strains belonging pathogenic, non-pathogenic intermediate spp. as well 46 other...
Leptospirosis is a zoonotic bacterial disease that affects more than one million people worldwide each year. Human infection acquired through direct or indirect contact with the urine of an infected animal. A wide range animals including rodents and livestock may shed Leptospira bacteria act as source for people. In Kilimanjaro Region northern Tanzania, leptospirosis important cause acute febrile illness, yet relatively little known about animal hosts in this area. The roles ruminant...
Genomic instability, which is a hallmark of cancer, generally thought to occur in the middle late stages tumourigenesis, following acquisition permissive molecular aberrations such as TP53 mutation or whole genome doubling. Tumours with somatic POLE exonuclease domain mutations are notable for their extreme genomic instability (their burden among highest human cancer), distinct mutational signature, lymphocytic infiltrate, and excellent prognosis. To what extent these characteristics...
S10-spc-α is a 17.5 kb cluster of 32 genes encoding ribosomal proteins. This locus has an unusual composition and organization in Leptospira interrogans. We demonstrate the highly conserved nature this region among diverse show its utility as phylogenetically informative region. Comparative analyses were performed by PCR using primer sets covering whole locus. Correctly sized fragments obtained from all L. interrogans strains tested for each set indicating that well species. Few differences...
Conventional diagnosis of leptospirosis and characterization Leptospira spp.relies mainly on serology.A major drawback serology in is that it needs sufficient levels anti-Leptospira antibodies, thus jeopardizing confirmation the early acute phase disease.The cross agglutinin absorption test (CAAT) determines serovars a technically demanding laborious method therefore only performed at few laboratories.Novel molecular diagnostic tests rely polymerase chain reaction (PCR).PCR perfectly...
Leptospirosis is a widespread but under-reported cause of morbidity and mortality. Global re-emergence leptospirosis has been associated with the growth informal urban settlements in which rodents are thought to be important reservoir hosts. Understanding multi-host epidemiology essential control prevent disease. A cross-sectional survey Kibera settlement Nairobi, Kenya was conducted September–October 2008 demonstrate presence pathogenic leptospires. real-time quantitative polymerase chain...
Leptospirosis is a growing public and veterinary health concern caused by pathogenic species of Leptospira. Rapid reliable laboratory tests for the direct detection leptospiral infections in animals are high demand not only to improve diagnosis but also understanding epidemiology disease. In this work we describe novel simple TaqMan-based multi-gene targeted real-time PCR approach able detect differentiate Leptospira interrogans, L. kirschneri, borgpeteresenii noguchii, which constitute most...
Detection of leptospires based on DNA amplification techniques is essential for the early diagnosis leptospirosis when anti-Leptospira antibodies are below detection limit most serological tests. In middle and low income countries where endemic, routine implementation real-time PCR financially technically challenging due to requirement expensive thermocycler equipment. this study we report development evaluation a novel isothermal recombinase polymerase assay (RPA) pathogenic Leptospira...
Escherichia coli (E. coli) is a widespread avian pathogen, usually considered as secondary pathogen for different infectious agents causing huge financial losses in the poultry industry. A total of 18 flocks out 30 broiler (60%) were positive E. infection. Based on Congo red (CR) binding assay, 15 identified pathogenic (83.3%) and 3 appeared non-pathogenic (16.6%). All subjected to vitro antibiotic sensitivity testing select most resistant isolates. Ten isolates representing ten molecular...
Background Leptospirosis has long been a major public health concern in the southwestern Indian Ocean. However, Madagascar, only few, old studies have provided indirect serological evidence of disease humans or animals. Methodology/Principal Findings We conducted large animal study focusing on small-mammal populations. Five field trapping surveys were carried out at five sites, from April 2008 to August 2009. Captures consisted Rattus norvegicus (35.8%), R. rattus (35.1%), Mus musculus...
Leptospirosis is an important zoonosis with widespread human health implications. The non-availability of accurate identification methods for the individualization different Leptospira outbreak investigations poses bountiful problems in disease control arena. We harnessed fluorescent amplified fragment length polymorphism analysis (FAFLP) and investigated its utility establishing genetic relationships among 271 isolates context species level assignments our global collection strains obtained...
Background Several sequence based genotyping schemes have been developed for Leptospira spp. The objective of this study was to genotype a collection clinical and reference isolates using the two most commonly used compare contrast results. Methods Findings A total 48 consisting L. interrogans (n = 40) kirschneri 8) were typed by 7 locus MLST scheme described Thaipadungpanit et al., 6 Ahmed (termed 7L 6L, respectively). Two not 6L because deletion three nucleotides in lipL32. remaining 46...
Leptospirosis is the most neglected widespread zoonosis worldwide. In Spain, leptospirosis reports in people and animals have increased lately. Cats can become infected with Leptospira, as well be chronic carriers. The aim of this study was to determine serological antibody prevalence against Leptospira sp., blood DNA, shedding DNA from pathogenic species urine cats Spain. Microagglutination tests (MAT) TaqMan real-time polymerase chain reaction (PCR) were performed. antibodies detected...
In Thailand, leptospirosis is considered an emerging disease in humans and animals. Many species can shed pathogenic Leptospira, including domestic cats (felis catus), which might be able to pose a risk humans. There are no studies on Leptospira infections but other countries, it was demonstrated that with high prevalences. The aims of this study were evaluate whether outdoor Thailand their urine, determine antibody prevalence factors associated infection. Two hundred sixty prospectively...
Three sets of 20 trinucleotides are preferentially associated with the reading frames and their 2 shifted both eukaryotic prokaryotic genes. These 3 circular codes. They allow retrieval any frame in genes (containing these code words), locally anywhere particular without start codons frame, automatically a few nucleotides. The noted X, which can deduce other codes by permutation, is information used for analysing frameshift genes, i.e. change during translation. This work studies signal...
Next-generation sequencing (NGS) efforts have established catalogs of mutations relevant to cancer development. However, the clinical utility this information remains largely unexplored. Here, we present results first eight patients recruited into a whole-genome (WGS) program in United Kingdom. We performed PCR-free WGS fresh frozen tumors and germline DNA at 75× 30×, respectively, using HiSeq2500 HTv4. Subtracted tumor VCFs paired germlines were subjected comprehensive analysis coding...
Fresh meat and poultry are easily contaminated with various contaminants especially if it is not properly handled preserved, leading to loss of quality potential public health problems.Food safety addresses the accidental contamination food products during processing or storage animal origin products.The main types hazards biological, chemicals, foreign objects.This unintentional slaughter can be reasonably anticipated based on type processing.This principle foundation Hazard Analysis...