A5037921470- Leptospirosis research and findings
- Analytical Chemistry and Chromatography
- Veterinary medicine and infectious diseases
- Mycobacterium research and diagnosis
- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Mass Spectrometry Techniques and Applications
- Bacterial biofilms and quorum sensing
- Fungal Biology and Applications
- Viral Infections and Vectors
- Bartonella species infections research
- Antimicrobial Resistance in Staphylococcus
- Diet and metabolism studies
- Colorectal Cancer Screening and Detection
- Chromatography in Natural Products
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Medical and Health Sciences Research
- Orthopedic Infections and Treatments
- Zoonotic diseases and public health
- Fermentation and Sensory Analysis
- Bacterial Identification and Susceptibility Testing
- Advanced Chemical Sensor Technologies
- Nutritional Studies and Diet
- Enterobacteriaceae and Cronobacter Research
University of Arkansas for Medical Sciences
2023-2024
Siriraj Hospital
2021-2024
Mahidol University
2021-2024
Chulalongkorn University
2017-2021
University of Bern
2021
Ludwig-Maximilians-Universität München
2019
Leptospirosis is an important zoonotic disease that often associated with animal carriers and contamination of the environment via infected urine. This study aimed to assess pathogenic leptospiral carriage in Nan province, a rural area Thailand where leptospirosis endemic. Samples from 20 villages were obtained during period 2013 2016, comprising urine samples collected asymptomatic people (n=37) domestic animals (n=342), environmental water (n=14). Leptospira cultured Ellinghauson...
Abstract Background Leptospirosis is a widespread zoonosis and has been recognized as re-emerging infectious disease in humans dogs, but prevalence of Leptospira shedding dogs Thailand unknown. The aim this study was to determine urinary Thailand, evaluate antibody by microscopic agglutination test (MAT) enzyme-linked immunosorbent assay (ELISA), assess risk factors for infection. In Northern, Northeastern, Central 273 stray ( n = 119) or client-owned 154) from rural 139) urban 134) areas...
In Thailand, leptospirosis is considered an emerging disease in humans and animals. Many species can shed pathogenic Leptospira, including domestic cats (felis catus), which might be able to pose a risk humans. There are no studies on Leptospira infections but other countries, it was demonstrated that with high prevalences. The aims of this study were evaluate whether outdoor Thailand their urine, determine antibody prevalence factors associated infection. Two hundred sixty prospectively...
Short-chain fatty acids (SCFAs) are involved in important physiological processes such as gut health and immune response, changes SCFA levels can be indicative of disease. Despite the importance SCFAs human disease, reference values for fecal plasma concentrations healthy individuals scarce. To address this gap current knowledge, we developed a simple reliable derivatization-free GC-TOFMS method quantifying individuals. We targeted six linear- seven branched-SCFAs, obtaining recoveries...
Leptospirosis is an emerging infectious disease worldwide that can cause high morbidity and mortality rates in humans animals. The causative spirochetes have reservoirs mammalian hosts, but there has been limited analysis of the genomes isolates recovered from aims this study were to characterize genomic features two Leptospira interrogans strains recently isolated asymptomatic dogs Thailand (strains CUDO5 CDUO8), perform comparative genome analyses with other strains. Molecular adaptive...
The collision cross-sections (CCS) measurement using ion mobility spectrometry (IMS) in combination with mass (MS) offers a great opportunity to increase confidence metabolite identification. However, owing the lack of sensitivity and resolution, IMS has an analytical challenge studying CCS values very low-molecular-weight metabolites (VLMs ≤ 250 Da). Here, we describe method ultrahigh-performance liquid chromatography (UPLC) coupled traveling wave mobility-quadrupole-time-of-flight...
The combination of ion mobility mass spectrometry (IM-MS) and chromatography is a valuable tool for identifying compounds in natural products. In this study, using an ultra-performance liquid system coupled to high-resolution quadrupole/traveling wave spectrometry/time-of-flight MS (UPLC-TWIMS-QTOF), we have established validated comprehensive TWCCSN2 database 112 plant specialized metabolites. included 15 that were isolated purified in-house are not commercially available. We obtained...
The accurate quantification of triterpenoids in Ganoderma lucidum mushroom the mycelium stage is challenging due to their low concentrations, interference from other possible isomers, and complex matrix. Here, a high-resolution quadrupole-time-of-flight mass spectrometry "multiple reaction monitoring" with target enhancement (HR-QTOF-MRM) method was developed quantify seven G. lucidum. performance this compared against an optimized QQQ-MRM method. HR-QTOF-MRM shown be capable distinguishing...
In 2014, Leptospira interrogans strain CUDO8 was isolated from the urine of an asymptomatic dog in Thailand. Here we report draft genome sequence this pathogenic bacterium.
Accurate taxonomic profiling of microbial taxa in a metagenomic sample is vital to gain insights into ecology. Recent advancements sequencing technologies have contributed tremendously toward understanding these microbes at species resolution through whole shotgun approach. In this study, we developed new bioinformatics tool, coverage-based analysis for identification microbiome (CAIM), accurate classification and quantification within both long- short-read samples using an alignment-based...
Leptospira interrogans serovar Bataviae is one of the serovars that can infect dogs. We report draft genome sequence strain D64, which was isolated from urine an asymptomatic dog in Pathum Thani, Thailand, 2017.
The rotationally averaged collision cross-section (CCS) determined by ion mobility-mass spectrometry (IM-MS) facilitates the identification of various biomolecules. Although machine learning (ML) models have recently emerged as a highly accurate approach for predicting CCS values, they rely on large data sets from instruments, calibrants, and setups, which can introduce additional errors. In this study, we identified validated that ion's polarizability mass-to-charge ratio (m/z) most...
Leptospirosis is a zoonotic disease of significant concern for human and animal health, with domestic animals, including dogs, acting as reservoirs infection. Serology widely used leptospirosis diagnosis, even though the standard microscopic agglutination test (MAT) using panel serovars lacks specificity can lead to detection limitations in certain regions. In this study, we aimed develop an antibody tool dogs indirect enzyme-linked immunosorbent assay (ELISA) set local serovar isolates,...
The environmental dissemination of antimicrobial resistant Enterobacteriaceae poses a remarkable threat to public health. This study investigates the presence and carbapenemase-producing (CPE) in 30 important water bodies selected according interconnection utilization by livestock community people central Thailand. From all samples, 6 isolates (20%) were identified as multi-drug CPE including one E. coli, K. pneumoniae four roggemkampii carrying blaNDM-5, blaNDM-1 blaIMI-1 genes...
Staphylococcus haemolyticus and hominis subsp. are common coagulase-negative staphylococcus opportunistic pathogens. In Thailand, the clinical strains S. 1864 48 384 371 have been recovered from sick dogs. These were methicillin resistant with nontypeable staphylococcal cassette chromosome mec (NT-SCCmec). The SCCmec element distribution in isolates dogs was analyzed using whole-genome sequencing, which revealed presence of different composite islands (CIs) gene structure. SCCmec-CIs...
Leptospira weilii belongs to the pathogenic group and is a causal agent of human animal leptospirosis in many world regions. L. can produce varied clinical presentations from asymptomatic through acute chronic infections occupy several ecological niches. Nevertheless, genomic feature genetic basis behind host adaptability remain elusive due limited information. Therefore, this study aimed examine complete circular genomes two new serogroup Mini strains (CUDO6 CUD13) recovered urine dogs...
Hintergrund In Thailand erkranken jedes Jahr 6,6 pro 100 000 Menschen an Leptospirose. Katzen können pathogene Leptospira species (spp.) mit dem Urin ausscheiden, in Taiwan sogar einer Prävalenz von 67,8%. Bisher gibt es keine Studien über Infektionen Leptospiren bei Thailand. Ziel der Studie war es, die Ausscheidung pathogener im und Antikörperprävalenz zu bestimmen Risikofaktoren analysieren. Material Methoden 260 Freigängerkatzen wurden prospektiv untersucht. Ihr wurde mittels für das...
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